Journal: Genome biology and evolution

Article Title: Horizontal Acquisition and Transcriptional Integration of Novel Genes in Mosquito-Associated Spiroplasma.

doi: 10.1093/gbe/evx244

Figure Lengend Snippet: FIG. 3.—Distribution of the RPKM values observed. (A) Spiroplasma diminutum. (B) Spiroplasma taiwanense. The median values prior to log-transfor- mation are labeled. The inner fence values (i.e., ends of the whiskers) represent the lowest value within 1.5 interquartile range of the lower quartile and the highest value within 1.5 interquartile range of the upper quartile.

Article Snippet: Complete genome sequence of Spiroplasma apis B31T (ATCC 33834), a bacterium associated with May disease of honeybees (Apis mellifera).

Techniques: Labeling

Journal: Genome biology and evolution

Article Title: Horizontal Acquisition and Transcriptional Integration of Novel Genes in Mosquito-Associated Spiroplasma.

doi: 10.1093/gbe/evx244

Figure Lengend Snippet: FIG. 4.—Gene expression levels in response to glucose availability. (A) Spiroplasma diminutum. (B) Spiroplasma taiwanense. The expression levels are measured in RPKM mapped reads (log2-transformed). All protein-coding genes and two highly expressed noncoding genes (i.e., tmRNA and rnpB) are shown. The genes exhibiting significant differential expressions between the two growth conditions are color-coded based on their COG functional category assignments. The remaining genes are represented by gray dots.

Article Snippet: Complete genome sequence of Spiroplasma apis B31T (ATCC 33834), a bacterium associated with May disease of honeybees (Apis mellifera).

Techniques: Gene Expression, Expressing, Transformation Assay, Functional Assay

Journal: Journal of Lipid Research

Article Title: Prophages divert Staphylococcus aureus defenses against host lipids

doi: 10.1016/j.jlr.2024.100693

Figure Lengend Snippet: Hlb activity and anti-FASII treatments enhance C18:2 incorporation in S. aureus PLs. TB3 (фNM3 in hlb ) and TB4 (no phage) strains were cultured in BHI medium supplemented with 10% mouse serum, 30 μM 1,3DG, with or without 0.5 μg/ml AFN-1252 (anti-FASII), and with or without 50 μM GW4869 (inhibitor of Hlb). Top left: bacterial growth rates in the absence of anti-FASII treatment, performed in glass tubes. Top right: growth kinetics in the presence of AFN-1252, measured in 96-well microtiter plates with OD600 reading every 10 min. Bottom: C18:2 incorporation with or without AFN-1252. C18:2 incorporation was determined in the TB4 and TB3 strains as described in Figure 2 B. Data are means ± standard deviations from independent experiments (n = 4). Statistical significance was determined by the Mann-Whitney test for on OD 600 /h and by the Wilcoxon Signed Rank test for incorporated C18:2; ∗, P ≤ 0.05. n.s: not significant.

Article Snippet: Stocks of trilinolein (TG18:2, 50 mM), triarachidonin (TG20:4, 10 mM), trieicosapentaenoin (TG20:5, 50 mM), tridocosahexaenoin (TG22:6, 50 mM), 1,2-dilinoleoylglycerol (1,2DG, 50 mM), 1,3-dilinoleoylglycerol (1,3DG, 50 mM), palmitic acid (C16, 100 mM), (Larodan Fine Chemicals), orlistat (38 mM, MedChemExpress), and the anti-FASII antibiotic AFN-1252 (1 mg/ml, MedChemExpress) were prepared in DMSO.

Techniques: Activity Assay, Cell Culture, MANN-WHITNEY