Journal: Cell Death and Differentiation

Article Title: p53 transcriptionally activates DCP1B to suppress tumor progression and enhance tumor sensitivity to PI3K blockade in non-small cell lung cancer

doi: 10.1038/s41418-025-01501-y

Figure Lengend Snippet: A , B WB assays assessing the expression levels of downstream proteins in A549 and H460 cells stably expressing shNC and shMAPK4. C , D WB assays assessing the expression levels of downstream proteins in A549 and H460 cells stably expressing PCDH and PCDH-MAPK4. E , F WB assays assessing the expression levels of downstream proteins in A549 and H460 cells stably expressing PCDH and PCDH-MAPK4 treated with or without PI3K inhibitor Alpelisib. G – J RT-Qpcr and WB assays validating the expression levels of MAPK4 in A549 and H460 treated with gradually increasing drug concentrations of p53-inducing agent APG-115 for 24 h. Data in G and I are represented as mean ± SD, n = 3.

Article Snippet: Nutlin-3, 5-fluorouracil (5-Fu), Cisplatin, Etoposide, APG-115, Alpelisib, Actinomycin D (Act.D), and Dimethyl sulfoxide (DMSO) were purchased from MedChemExpress (MCE, Shanghai, China).

Techniques: Expressing, Stable Transfection, Quantitative RT-PCR

Journal: Cell Death and Differentiation

Article Title: p53 transcriptionally activates DCP1B to suppress tumor progression and enhance tumor sensitivity to PI3K blockade in non-small cell lung cancer

doi: 10.1038/s41418-025-01501-y

Figure Lengend Snippet: A – H Drug-sensitivity and the combination index (CI) of 2 drugs assays showing the synergistic inhibition effect of p53 agonist and PI3K inhibitor in A549 and H460 cells in vitro. Data in A , C , E and G are represented as mean ± SD, n = 3. I – M A549 cells were inoculated into BALB/c female nude mice. After 14 days of injection, mice were randomly divided into four groups including the control group, APG-115 group, Alpelisib group, and the co-drug group. After 3 weeks, mice were sacrificed and xenograft tumors were removed. Representative mice weight, tumor volume, tumor weight, and tumor images are shown. N The proposed working model. Data in K and L are represented as mean ± SD, n = 7.

Article Snippet: Nutlin-3, 5-fluorouracil (5-Fu), Cisplatin, Etoposide, APG-115, Alpelisib, Actinomycin D (Act.D), and Dimethyl sulfoxide (DMSO) were purchased from MedChemExpress (MCE, Shanghai, China).

Techniques: Inhibition, In Vitro, Injection, Control

Journal: International Journal of Molecular Sciences

Article Title: Quantitative Proteomic Analysis of Escherichia coli Heat-Labile Toxin B Subunit (LTB) with Enterovirus 71 (EV71) Subunit VP1

doi: 10.3390/ijms17091419

Figure Lengend Snippet: Immune system processing related differential proteins (21proteins).

Article Snippet: The target proteins were detected with the antibodies of EDIL3 (EGF-like repeat and discoidin I-like domain-containing protein 3; cat# 12580-1-AP), STAT1 (signal transducer and activator of transcription 1, cat# 10144-2-AP), PRDX1 (peroxiredoxin-1, cat# 15816-1-AP), HSPA4 (heat shock 70 kDa protein 4, cat#21206-1-AP), GSTO1 (glutathione S -transferase ω-1, cat# 15124-1-AP), and β-Actin (14395-1-AP) (Proteintech, Wuhan, China), followed by incubation with a secondary antibody conjugated with HRP.

Techniques: Activity Assay, Immunopeptidomics, Virus, Infection, Control, Cell Differentiation

Journal: Cell Reports Medicine

Article Title: Venetoclax acts as an immunometabolic modulator to potentiate adoptive NK cell immunotherapy against leukemia

doi: 10.1016/j.xcrm.2024.101580

Figure Lengend Snippet:

Article Snippet: Lisaftoclax , MedChemExpress , Cat# HY-129179.

Techniques: Recombinant, Staining, Blocking Assay, CCK-8 Assay, Western Blot, Cell Isolation, Software