Journal: Oncology Letters

Article Title: CD73 promotes colitis-associated tumorigenesis in mice

doi: 10.3892/ol.2020.11670

Figure Lengend Snippet: AOM/DSS-induced CAT model in C57BL/6J mice. (A) Experimental protocol of the CAT model. Black box indicate the week of 2% DSS in the drinking water while white box indicate the week of normal drinking water. Mice in the control group were intraperitoneally injected with PBS and received pure water for 9 weeks. (B) Changes in mouse body weight in the first cycle. (C) A total of 6 mice in the control group, 9 mice in the APCP group, 6 mice in the model group and 4 mice in the NECA group survived during the period of experiment. (D) Mouse body weight changes during the entire experimental period. APCP treatment ameliorated and NECA treatment aggravated weight loss compared with PBS treatment in the experimental groups. Values are expressed as the mean ± SEM. *P<0.05. AOM, azoxymethane; DSS, dextran sulfate sodium; CAT, colitis-associated tumorigenesis; APCP, adenosine 5′-(α,β-methylene) diphosphate; NECA, 1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-β-D-ribofuranuronamide.

Article Snippet: Azoxymethane (AOM), APCP and NECA were purchased from Sigma-Aldrich; Merck-KGaA.

Techniques: Control, Injection

Journal: Oncology Letters

Article Title: CD73 promotes colitis-associated tumorigenesis in mice

doi: 10.3892/ol.2020.11670

Figure Lengend Snippet: APCP attenuates and NECA aggravates azoxymethane/dextran sulfate sodium-induced colitis-associated tumorigenesis. (A) Representative images of mouse colon length. (B) Comparison of colon length. (C) Macroscopic inspection of mouse colonic tumor number and diameter. Tumors are indicated by red arrows. Comparison of (D) tumor number, (E) diameter and (F) tumor burden. Values are expressed as the mean ± SEM. *P<0.05. APCP, adenosine 5′-(α,β-methylene) diphosphate; NECA, 1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-β-D-ribofuranuronamide.

Article Snippet: Azoxymethane (AOM), APCP and NECA were purchased from Sigma-Aldrich; Merck-KGaA.

Techniques: Comparison

Journal: Oncology Letters

Article Title: CD73 promotes colitis-associated tumorigenesis in mice

doi: 10.3892/ol.2020.11670

Figure Lengend Snippet: APCP decreases and NECA increases histological damage and expression of inflammatory cytokines in colon tissue. (A) Microscopic examination of colonic tissue by hematoxylin and eosin staining. (B) Comparison of histopathological evaluation score. Quantitative PCR was used to evaluate the expression of inflammatory cytokines (C) TNF-α, (D) IL-6, (E) TNF-β and (F) IL-10 in colonic tissue. Values are expressed as the mean ± SEM. *P<0.05. APCP, adenosine 5′-(α,β-methylene) diphosphate; NECA, 1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-β-D-ribofuranuronamide; TNF, tumor necrosis factor; IL, interleukin.

Article Snippet: Azoxymethane (AOM), APCP and NECA were purchased from Sigma-Aldrich; Merck-KGaA.

Techniques: Expressing, Staining, Comparison, Real-time Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: CD73 promotes colitis-associated tumorigenesis in mice

doi: 10.3892/ol.2020.11670

Figure Lengend Snippet: Identification of DEGs and genes associated with colitis-associated tumorigenesis by RNA-sequencing. KEGG pathway analysis indicated the different pathway distributions of the DEGs between (A) model vs. APCP, (B) model vs. NECA and (C) NECA vs. APCP experimental groups. (D) Quantitative PCR was used to validate the expression levels of ALOX15, Bcl2l15 and Nat8l. Values are expressed as the mean ± SEM. *P<0.05. DEGs, differentially-expressed genes; GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; APCP, adenosine 5′-(α,β-methylene) diphosphate; NECA, 1-(6-amino-9H-purin-9-yl)-1-deoxy-N-ethyl-β-D-ribofuranuronamide; ALOX15, arachidonate 15-lipoxygenase.

Article Snippet: Azoxymethane (AOM), APCP and NECA were purchased from Sigma-Aldrich; Merck-KGaA.

Techniques: RNA Sequencing, Real-time Polymerase Chain Reaction, Expressing

Journal: Scientific Reports

Article Title: The interaction of mast cells with membranes from lung cancer cells induces the release of extracellular vesicles with a unique miRNA signature

doi: 10.1038/s41598-023-48435-4

Figure Lengend Snippet: The phosphorylation of ERK 1/2 in HMC-1 cells stimulated by H1299-derived membranes was evaluated upon inhibition of MEK, PI3K, PKC, A3R, and CD73. HMC-1 cells (1 × 10 6 cells/ml) were pretreated with the indicated inhibitors (3 µM U0126, 10 µM LY 294002, 1 µM GO 9676, 100 nM MRS 1220, and 5 µM APCP, respectively) for 30 min. Subsequently, the cells were exposed to 10 µg/ml H1299-derived membranes for 1 min. Cellular lysates underwent SDS-PAGE separation and were subjected to probing with an anti-p-ERK 1/2 antibody and subsequent reprobed using an anti-total ERK2 antibody. The phospho-ERK 1/2 and total ERK bands were determined using ImageJ software, and the relative pixel densities (phosphorylated/total) were calculated and normalized to those of untreated (UT) cells. The data presented reflect the average of three distinct experiments and are shown as the mean ± SEM (error bars). A p value less than 0.05 was considered significant and denoted by *, while ** indicates a p value less than 0.01.

Article Snippet: The PKC inhibitor GO6976, PI3K inhibitor LY294002, MEK inhibitor U0126, and CD73 inhibitor APCP were purchased from A.G. Scientific Inc.

Techniques: Derivative Assay, Inhibition, SDS Page, Software