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Image Search Results
Journal: bioRxiv
Article Title: Perturbation of ubiquitin homeostasis promotes macrophage oxidative defenses
doi: 10.1101/276964
Figure Lengend Snippet: A). Structures of compounds DUB inh -Biotin and ΔCN-biotin. The arrow highlights the key cyano group. B). RAW264. 7 whole cell lysates were incubated with DUB inh -Biotin or ΔCN-biotin before immunoprecipitation using streptavidin-coated beads. The retained proteins were analyzed by SDS-PAGE and revealed by silver stain. C). Pull downs and corresponding inputs were immunoblotted for MYSM1, DUBA, Ataxin3, USP14, YOD1 and USP25. Data are representative of three independent experiments.
Article Snippet: The primary antibodies used for immunoblotting were anti-ubiquitin, clone P4D1 (sc-8017), anti-USP14 (sc-100630), anti-USP25 (sc-398414) and anti-gp91 phox (sc-130543) from Santa Cruz, anti-YOD1 (ARP67915) from Aviva Systems Biology, anti-Otud5 (DUBA, 21002-1-AP) from
Techniques: Incubation, Immunoprecipitation, SDS Page, Silver Staining