Journal: eLife

Article Title: Single-cell profiling of trabecular meshwork identifies mitochondrial dysfunction in a glaucoma model that is protected by vitamin B3 treatment

doi: 10.7554/eLife.107161

Figure Lengend Snippet: ( A ) The expression levels of various anterior segment cell type marker genes in the integrated B6 and 129 data are depicted on a dot plot. These marker genes are generally accepted to be specific to individual cell types. Epithelial cells ( Krt14 ); neurons ( Rho ); endothelial cells ( Egfl7 ); ciliary body and iris cells ( Tyrp1 ); and immune cells ( Cd52 ). Cluster 1 expressed multiple TM marker genes including Myoc , Acta2 (encodes α-SMA), Pitx2 , and Tfap2b . Although some of the neurons may be limbal, it is possible that others are retinal contamination. ( B–C ) Heatmaps of differentially expressed genes across all limbal tissue cell clusters ( B ) and across the subclusters of cluster 1 ( C ).

Article Snippet: Antibody , Rabbit polyclonal TFAP2B , Novus Biologicals , NBP1-89063 , IF sections (1:200).

Techniques: Expressing, Marker

Journal: eLife

Article Title: Single-cell profiling of trabecular meshwork identifies mitochondrial dysfunction in a glaucoma model that is protected by vitamin B3 treatment

doi: 10.7554/eLife.107161

Figure Lengend Snippet: Although there is variability in expression between sections, the aggregate of all sections reveals the biased distributions of TM cell subtypes as summarized in . Here we focus on TM expression. However, some of the markers are also expressed outside of the TM. For example, Edn3 is expressed in vasculature, while both Lypd1 and Inmt are expressed at low levels in scleral and iris cells (but are higher in TM cells). Markers assessed by IF: MYOC, CHIL1, CRYM, α-SMA, TFAP2B. Markers assessed by ISH: Inmt , Edn3 , Lypd1 . All scale bars: 50 µm.

Article Snippet: Antibody , Rabbit polyclonal TFAP2B , Novus Biologicals , NBP1-89063 , IF sections (1:200).

Techniques: Expressing