ap1 Search Results


c fos  (Proteintech)
94
Proteintech c fos
Astrocytic PCBP1 enhances glutamatergic neuronal activity and neurophysiology. <t>a)</t> <t>c‐Fos</t> expression in vHip of mice following CUMS. Above: Representative images and quantification of c‐Fos‐positive glutamatergic neurons. Below: Representative images and quantification of c‐Fos‐positive GABAergic neurons. Scale bars = 50 µm. n = 9 slices from 3 animals per group. b) Timeline of fiber photometry recording Ca 2+ and glutamate signals from vHip c) Left: Representative images validate GCaMP6s expression in vHip. Scale bar = 500 µm. Right: Representative images showing the overlap between GCaMP6s‐expressing cells (green) and glutamatergic neurons (violet). Scale bar = 50 µm. d) Representative images of c‐Fos‐positive glutamatergic neurons. Scale bars = 100 or 20 µm. e) Statistical analysis of c‐Fos co‐labeled glutamatergic neuron in vHip of mice. f) Schematic of fiber photometry recording in TST and representative Ca 2+ signals photometric traces in response to the TST during struggling and immobility phases. Scale bars = 10 s. g) Ca 2 ⁺ signal recording in vHip during TST. Left: Representative heatmaps showing Ca 2 ⁺ signal in response to TST during struggling. Right: Z‐score traces and quantification of average Z‐scores during TST. n = 5 mice per group. h) Representative images of the AAV vectors engineered to express the glutamate sensor iGluSnFR under the hSyn promoter in the vHip. Scale bars = 500 µm / 50 µm. i) Representative trace of glutamate signal event detection during TST. Red dots indicate detected events. j) Event‐based quantification. Event frequency (left), AUC (middle) and decay tau (right). n = 5 mice per group. k) Timeline of electrophysiological recordings from vHip glutamatergic neurons. l) Quantification of mEPSC frequency (left) and amplitude (right) recorded from vHip glutamatergic neurons. n = 10–12 cells from 3 mice per group. m) Left: Evoked firing rates of action potentials recorded from eGFP⁺ neurons in the vHip. Right: Quantifications of rheobase and resting membrane potential recorded from eGFP + neurons in vHip. n = 10 cells from 3 mice per group. Statistical analyses included unpaired t‐test, one‐way/two‐way ANOVA followed by Tukey's post hoc test and Kruskal‐Wallis test. Data are presented as means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
C Fos, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ap1/pmc12915190-366-33-43?v=Proteintech
Average 94 stars, based on 1 article reviews
c fos - by Bioz Stars, 2026-07
94/100 stars
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antibodies against phospho c jun n terminal kinase jnk  (Boster Bio)
93
Boster Bio antibodies against phospho c jun n terminal kinase jnk
Astrocytic PCBP1 enhances glutamatergic neuronal activity and neurophysiology. <t>a)</t> <t>c‐Fos</t> expression in vHip of mice following CUMS. Above: Representative images and quantification of c‐Fos‐positive glutamatergic neurons. Below: Representative images and quantification of c‐Fos‐positive GABAergic neurons. Scale bars = 50 µm. n = 9 slices from 3 animals per group. b) Timeline of fiber photometry recording Ca 2+ and glutamate signals from vHip c) Left: Representative images validate GCaMP6s expression in vHip. Scale bar = 500 µm. Right: Representative images showing the overlap between GCaMP6s‐expressing cells (green) and glutamatergic neurons (violet). Scale bar = 50 µm. d) Representative images of c‐Fos‐positive glutamatergic neurons. Scale bars = 100 or 20 µm. e) Statistical analysis of c‐Fos co‐labeled glutamatergic neuron in vHip of mice. f) Schematic of fiber photometry recording in TST and representative Ca 2+ signals photometric traces in response to the TST during struggling and immobility phases. Scale bars = 10 s. g) Ca 2 ⁺ signal recording in vHip during TST. Left: Representative heatmaps showing Ca 2 ⁺ signal in response to TST during struggling. Right: Z‐score traces and quantification of average Z‐scores during TST. n = 5 mice per group. h) Representative images of the AAV vectors engineered to express the glutamate sensor iGluSnFR under the hSyn promoter in the vHip. Scale bars = 500 µm / 50 µm. i) Representative trace of glutamate signal event detection during TST. Red dots indicate detected events. j) Event‐based quantification. Event frequency (left), AUC (middle) and decay tau (right). n = 5 mice per group. k) Timeline of electrophysiological recordings from vHip glutamatergic neurons. l) Quantification of mEPSC frequency (left) and amplitude (right) recorded from vHip glutamatergic neurons. n = 10–12 cells from 3 mice per group. m) Left: Evoked firing rates of action potentials recorded from eGFP⁺ neurons in the vHip. Right: Quantifications of rheobase and resting membrane potential recorded from eGFP + neurons in vHip. n = 10 cells from 3 mice per group. Statistical analyses included unpaired t‐test, one‐way/two‐way ANOVA followed by Tukey's post hoc test and Kruskal‐Wallis test. Data are presented as means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Antibodies Against Phospho C Jun N Terminal Kinase Jnk, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ap1/pmc08784769-44-0-14?v=Boster+Bio
Average 93 stars, based on 1 article reviews
antibodies against phospho c jun n terminal kinase jnk - by Bioz Stars, 2026-07
93/100 stars
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human c fos  (OriGene)
93
OriGene human c fos
Astrocytic PCBP1 enhances glutamatergic neuronal activity and neurophysiology. <t>a)</t> <t>c‐Fos</t> expression in vHip of mice following CUMS. Above: Representative images and quantification of c‐Fos‐positive glutamatergic neurons. Below: Representative images and quantification of c‐Fos‐positive GABAergic neurons. Scale bars = 50 µm. n = 9 slices from 3 animals per group. b) Timeline of fiber photometry recording Ca 2+ and glutamate signals from vHip c) Left: Representative images validate GCaMP6s expression in vHip. Scale bar = 500 µm. Right: Representative images showing the overlap between GCaMP6s‐expressing cells (green) and glutamatergic neurons (violet). Scale bar = 50 µm. d) Representative images of c‐Fos‐positive glutamatergic neurons. Scale bars = 100 or 20 µm. e) Statistical analysis of c‐Fos co‐labeled glutamatergic neuron in vHip of mice. f) Schematic of fiber photometry recording in TST and representative Ca 2+ signals photometric traces in response to the TST during struggling and immobility phases. Scale bars = 10 s. g) Ca 2 ⁺ signal recording in vHip during TST. Left: Representative heatmaps showing Ca 2 ⁺ signal in response to TST during struggling. Right: Z‐score traces and quantification of average Z‐scores during TST. n = 5 mice per group. h) Representative images of the AAV vectors engineered to express the glutamate sensor iGluSnFR under the hSyn promoter in the vHip. Scale bars = 500 µm / 50 µm. i) Representative trace of glutamate signal event detection during TST. Red dots indicate detected events. j) Event‐based quantification. Event frequency (left), AUC (middle) and decay tau (right). n = 5 mice per group. k) Timeline of electrophysiological recordings from vHip glutamatergic neurons. l) Quantification of mEPSC frequency (left) and amplitude (right) recorded from vHip glutamatergic neurons. n = 10–12 cells from 3 mice per group. m) Left: Evoked firing rates of action potentials recorded from eGFP⁺ neurons in the vHip. Right: Quantifications of rheobase and resting membrane potential recorded from eGFP + neurons in vHip. n = 10 cells from 3 mice per group. Statistical analyses included unpaired t‐test, one‐way/two‐way ANOVA followed by Tukey's post hoc test and Kruskal‐Wallis test. Data are presented as means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Human C Fos, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ap1/pmc04887844-27-1-9?v=OriGene
Average 93 stars, based on 1 article reviews
human c fos - by Bioz Stars, 2026-07
93/100 stars
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1 chlorobutane  (Thermo Fisher)
93
Thermo Fisher 1 chlorobutane
Astrocytic PCBP1 enhances glutamatergic neuronal activity and neurophysiology. <t>a)</t> <t>c‐Fos</t> expression in vHip of mice following CUMS. Above: Representative images and quantification of c‐Fos‐positive glutamatergic neurons. Below: Representative images and quantification of c‐Fos‐positive GABAergic neurons. Scale bars = 50 µm. n = 9 slices from 3 animals per group. b) Timeline of fiber photometry recording Ca 2+ and glutamate signals from vHip c) Left: Representative images validate GCaMP6s expression in vHip. Scale bar = 500 µm. Right: Representative images showing the overlap between GCaMP6s‐expressing cells (green) and glutamatergic neurons (violet). Scale bar = 50 µm. d) Representative images of c‐Fos‐positive glutamatergic neurons. Scale bars = 100 or 20 µm. e) Statistical analysis of c‐Fos co‐labeled glutamatergic neuron in vHip of mice. f) Schematic of fiber photometry recording in TST and representative Ca 2+ signals photometric traces in response to the TST during struggling and immobility phases. Scale bars = 10 s. g) Ca 2 ⁺ signal recording in vHip during TST. Left: Representative heatmaps showing Ca 2 ⁺ signal in response to TST during struggling. Right: Z‐score traces and quantification of average Z‐scores during TST. n = 5 mice per group. h) Representative images of the AAV vectors engineered to express the glutamate sensor iGluSnFR under the hSyn promoter in the vHip. Scale bars = 500 µm / 50 µm. i) Representative trace of glutamate signal event detection during TST. Red dots indicate detected events. j) Event‐based quantification. Event frequency (left), AUC (middle) and decay tau (right). n = 5 mice per group. k) Timeline of electrophysiological recordings from vHip glutamatergic neurons. l) Quantification of mEPSC frequency (left) and amplitude (right) recorded from vHip glutamatergic neurons. n = 10–12 cells from 3 mice per group. m) Left: Evoked firing rates of action potentials recorded from eGFP⁺ neurons in the vHip. Right: Quantifications of rheobase and resting membrane potential recorded from eGFP + neurons in vHip. n = 10 cells from 3 mice per group. Statistical analyses included unpaired t‐test, one‐way/two‐way ANOVA followed by Tukey's post hoc test and Kruskal‐Wallis test. Data are presented as means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
1 Chlorobutane, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ap1/pmc03836428-41-18-21?v=Thermo+Fisher
Average 93 stars, based on 1 article reviews
1 chlorobutane - by Bioz Stars, 2026-07
93/100 stars
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1 tetradecene  (Thermo Fisher)
93
Thermo Fisher 1 tetradecene
Astrocytic PCBP1 enhances glutamatergic neuronal activity and neurophysiology. <t>a)</t> <t>c‐Fos</t> expression in vHip of mice following CUMS. Above: Representative images and quantification of c‐Fos‐positive glutamatergic neurons. Below: Representative images and quantification of c‐Fos‐positive GABAergic neurons. Scale bars = 50 µm. n = 9 slices from 3 animals per group. b) Timeline of fiber photometry recording Ca 2+ and glutamate signals from vHip c) Left: Representative images validate GCaMP6s expression in vHip. Scale bar = 500 µm. Right: Representative images showing the overlap between GCaMP6s‐expressing cells (green) and glutamatergic neurons (violet). Scale bar = 50 µm. d) Representative images of c‐Fos‐positive glutamatergic neurons. Scale bars = 100 or 20 µm. e) Statistical analysis of c‐Fos co‐labeled glutamatergic neuron in vHip of mice. f) Schematic of fiber photometry recording in TST and representative Ca 2+ signals photometric traces in response to the TST during struggling and immobility phases. Scale bars = 10 s. g) Ca 2 ⁺ signal recording in vHip during TST. Left: Representative heatmaps showing Ca 2 ⁺ signal in response to TST during struggling. Right: Z‐score traces and quantification of average Z‐scores during TST. n = 5 mice per group. h) Representative images of the AAV vectors engineered to express the glutamate sensor iGluSnFR under the hSyn promoter in the vHip. Scale bars = 500 µm / 50 µm. i) Representative trace of glutamate signal event detection during TST. Red dots indicate detected events. j) Event‐based quantification. Event frequency (left), AUC (middle) and decay tau (right). n = 5 mice per group. k) Timeline of electrophysiological recordings from vHip glutamatergic neurons. l) Quantification of mEPSC frequency (left) and amplitude (right) recorded from vHip glutamatergic neurons. n = 10–12 cells from 3 mice per group. m) Left: Evoked firing rates of action potentials recorded from eGFP⁺ neurons in the vHip. Right: Quantifications of rheobase and resting membrane potential recorded from eGFP + neurons in vHip. n = 10 cells from 3 mice per group. Statistical analyses included unpaired t‐test, one‐way/two‐way ANOVA followed by Tukey's post hoc test and Kruskal‐Wallis test. Data are presented as means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
1 Tetradecene, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ap1/10__1021_slash_acscatal__7b00393-46-34-37?v=Thermo+Fisher
Average 93 stars, based on 1 article reviews
1 tetradecene - by Bioz Stars, 2026-07
93/100 stars
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anti c fos antibody proteintech 26192 1 ap  (Proteintech)
94
Proteintech anti c fos antibody proteintech 26192 1 ap
Astrocytic PCBP1 enhances glutamatergic neuronal activity and neurophysiology. <t>a)</t> <t>c‐Fos</t> expression in vHip of mice following CUMS. Above: Representative images and quantification of c‐Fos‐positive glutamatergic neurons. Below: Representative images and quantification of c‐Fos‐positive GABAergic neurons. Scale bars = 50 µm. n = 9 slices from 3 animals per group. b) Timeline of fiber photometry recording Ca 2+ and glutamate signals from vHip c) Left: Representative images validate GCaMP6s expression in vHip. Scale bar = 500 µm. Right: Representative images showing the overlap between GCaMP6s‐expressing cells (green) and glutamatergic neurons (violet). Scale bar = 50 µm. d) Representative images of c‐Fos‐positive glutamatergic neurons. Scale bars = 100 or 20 µm. e) Statistical analysis of c‐Fos co‐labeled glutamatergic neuron in vHip of mice. f) Schematic of fiber photometry recording in TST and representative Ca 2+ signals photometric traces in response to the TST during struggling and immobility phases. Scale bars = 10 s. g) Ca 2 ⁺ signal recording in vHip during TST. Left: Representative heatmaps showing Ca 2 ⁺ signal in response to TST during struggling. Right: Z‐score traces and quantification of average Z‐scores during TST. n = 5 mice per group. h) Representative images of the AAV vectors engineered to express the glutamate sensor iGluSnFR under the hSyn promoter in the vHip. Scale bars = 500 µm / 50 µm. i) Representative trace of glutamate signal event detection during TST. Red dots indicate detected events. j) Event‐based quantification. Event frequency (left), AUC (middle) and decay tau (right). n = 5 mice per group. k) Timeline of electrophysiological recordings from vHip glutamatergic neurons. l) Quantification of mEPSC frequency (left) and amplitude (right) recorded from vHip glutamatergic neurons. n = 10–12 cells from 3 mice per group. m) Left: Evoked firing rates of action potentials recorded from eGFP⁺ neurons in the vHip. Right: Quantifications of rheobase and resting membrane potential recorded from eGFP + neurons in vHip. n = 10 cells from 3 mice per group. Statistical analyses included unpaired t‐test, one‐way/two‐way ANOVA followed by Tukey's post hoc test and Kruskal‐Wallis test. Data are presented as means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Anti C Fos Antibody Proteintech 26192 1 Ap, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ap1/pmc11466963__41467_2024_53119_MOESM1_ESM-63-33-35?v=Proteintech
Average 94 stars, based on 1 article reviews
anti c fos antibody proteintech 26192 1 ap - by Bioz Stars, 2026-07
94/100 stars
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ifitm2  (Proteintech)
93
Proteintech ifitm2
Astrocytic PCBP1 enhances glutamatergic neuronal activity and neurophysiology. <t>a)</t> <t>c‐Fos</t> expression in vHip of mice following CUMS. Above: Representative images and quantification of c‐Fos‐positive glutamatergic neurons. Below: Representative images and quantification of c‐Fos‐positive GABAergic neurons. Scale bars = 50 µm. n = 9 slices from 3 animals per group. b) Timeline of fiber photometry recording Ca 2+ and glutamate signals from vHip c) Left: Representative images validate GCaMP6s expression in vHip. Scale bar = 500 µm. Right: Representative images showing the overlap between GCaMP6s‐expressing cells (green) and glutamatergic neurons (violet). Scale bar = 50 µm. d) Representative images of c‐Fos‐positive glutamatergic neurons. Scale bars = 100 or 20 µm. e) Statistical analysis of c‐Fos co‐labeled glutamatergic neuron in vHip of mice. f) Schematic of fiber photometry recording in TST and representative Ca 2+ signals photometric traces in response to the TST during struggling and immobility phases. Scale bars = 10 s. g) Ca 2 ⁺ signal recording in vHip during TST. Left: Representative heatmaps showing Ca 2 ⁺ signal in response to TST during struggling. Right: Z‐score traces and quantification of average Z‐scores during TST. n = 5 mice per group. h) Representative images of the AAV vectors engineered to express the glutamate sensor iGluSnFR under the hSyn promoter in the vHip. Scale bars = 500 µm / 50 µm. i) Representative trace of glutamate signal event detection during TST. Red dots indicate detected events. j) Event‐based quantification. Event frequency (left), AUC (middle) and decay tau (right). n = 5 mice per group. k) Timeline of electrophysiological recordings from vHip glutamatergic neurons. l) Quantification of mEPSC frequency (left) and amplitude (right) recorded from vHip glutamatergic neurons. n = 10–12 cells from 3 mice per group. m) Left: Evoked firing rates of action potentials recorded from eGFP⁺ neurons in the vHip. Right: Quantifications of rheobase and resting membrane potential recorded from eGFP + neurons in vHip. n = 10 cells from 3 mice per group. Statistical analyses included unpaired t‐test, one‐way/two‐way ANOVA followed by Tukey's post hoc test and Kruskal‐Wallis test. Data are presented as means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
Ifitm2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ap1/pmc04602366__mmc1-64-2-11?v=Proteintech
Average 93 stars, based on 1 article reviews
ifitm2 - by Bioz Stars, 2026-07
93/100 stars
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ap1  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology ap1
A, <t>AP1</t> DNA-binding activity of 25 microg of nuclear proteins. Nuclear extracts from NFRKB-transfected HEK cells were incubated with the wild-type AP1 oligonucleotide (Wt AP1) or with mutant-type AP1(Mut AP1) oligonucleotide. B, Expression of c-fos and c-jun. Immunoblots were performed with 50 microg of proteins. C, HEK cells were transiently co-transfected with the NFRKB or empty vector (Ev), and AP1-Luc reporter plasmid. The luciferase activity was measured using the “Dual Luciferase reporter Assay”. The data are presented as relative luciferase activity (firefly luciferase/the renilla luciferase). Statistical analyses were carried out on data from five independent experiments using the one-way Anova (** p <0.015; Kruskal-Wallis test).
Ap1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ap1/pmc03264618-179-21-29?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 1 article reviews
ap1 - by Bioz Stars, 2026-07
93/100 stars
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rg207004  (OriGene)
90
OriGene rg207004
A, <t>AP1</t> DNA-binding activity of 25 microg of nuclear proteins. Nuclear extracts from NFRKB-transfected HEK cells were incubated with the wild-type AP1 oligonucleotide (Wt AP1) or with mutant-type AP1(Mut AP1) oligonucleotide. B, Expression of c-fos and c-jun. Immunoblots were performed with 50 microg of proteins. C, HEK cells were transiently co-transfected with the NFRKB or empty vector (Ev), and AP1-Luc reporter plasmid. The luciferase activity was measured using the “Dual Luciferase reporter Assay”. The data are presented as relative luciferase activity (firefly luciferase/the renilla luciferase). Statistical analyses were carried out on data from five independent experiments using the one-way Anova (** p <0.015; Kruskal-Wallis test).
Rg207004, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ap1/pmc06642224-140-5-6?v=OriGene
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rg207004 - by Bioz Stars, 2026-07
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1 2 dichloroethane  (Thermo Fisher)
94
Thermo Fisher 1 2 dichloroethane
A, <t>AP1</t> DNA-binding activity of 25 microg of nuclear proteins. Nuclear extracts from NFRKB-transfected HEK cells were incubated with the wild-type AP1 oligonucleotide (Wt AP1) or with mutant-type AP1(Mut AP1) oligonucleotide. B, Expression of c-fos and c-jun. Immunoblots were performed with 50 microg of proteins. C, HEK cells were transiently co-transfected with the NFRKB or empty vector (Ev), and AP1-Luc reporter plasmid. The luciferase activity was measured using the “Dual Luciferase reporter Assay”. The data are presented as relative luciferase activity (firefly luciferase/the renilla luciferase). Statistical analyses were carried out on data from five independent experiments using the one-way Anova (** p <0.015; Kruskal-Wallis test).
1 2 Dichloroethane, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ap1/10__1016_slash_j__elecom__2017__11__001-45-5-10?v=Thermo+Fisher
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1 2 dichloroethane - by Bioz Stars, 2026-07
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1 butene  (Thermo Fisher)
94
Thermo Fisher 1 butene
A, <t>AP1</t> DNA-binding activity of 25 microg of nuclear proteins. Nuclear extracts from NFRKB-transfected HEK cells were incubated with the wild-type AP1 oligonucleotide (Wt AP1) or with mutant-type AP1(Mut AP1) oligonucleotide. B, Expression of c-fos and c-jun. Immunoblots were performed with 50 microg of proteins. C, HEK cells were transiently co-transfected with the NFRKB or empty vector (Ev), and AP1-Luc reporter plasmid. The luciferase activity was measured using the “Dual Luciferase reporter Assay”. The data are presented as relative luciferase activity (firefly luciferase/the renilla luciferase). Statistical analyses were carried out on data from five independent experiments using the one-way Anova (** p <0.015; Kruskal-Wallis test).
1 Butene, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ap1/pmc09023488-90-2-31?v=Thermo+Fisher
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1 butene - by Bioz Stars, 2026-07
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lysis buffer qiagen ap1 buffer  (Qiagen)
95
Qiagen lysis buffer qiagen ap1 buffer
A, <t>AP1</t> DNA-binding activity of 25 microg of nuclear proteins. Nuclear extracts from NFRKB-transfected HEK cells were incubated with the wild-type AP1 oligonucleotide (Wt AP1) or with mutant-type AP1(Mut AP1) oligonucleotide. B, Expression of c-fos and c-jun. Immunoblots were performed with 50 microg of proteins. C, HEK cells were transiently co-transfected with the NFRKB or empty vector (Ev), and AP1-Luc reporter plasmid. The luciferase activity was measured using the “Dual Luciferase reporter Assay”. The data are presented as relative luciferase activity (firefly luciferase/the renilla luciferase). Statistical analyses were carried out on data from five independent experiments using the one-way Anova (** p <0.015; Kruskal-Wallis test).
Lysis Buffer Qiagen Ap1 Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ap1/pmc02518145-34-14-17?v=Qiagen
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lysis buffer qiagen ap1 buffer - by Bioz Stars, 2026-07
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Image Search Results


Astrocytic PCBP1 enhances glutamatergic neuronal activity and neurophysiology. a) c‐Fos expression in vHip of mice following CUMS. Above: Representative images and quantification of c‐Fos‐positive glutamatergic neurons. Below: Representative images and quantification of c‐Fos‐positive GABAergic neurons. Scale bars = 50 µm. n = 9 slices from 3 animals per group. b) Timeline of fiber photometry recording Ca 2+ and glutamate signals from vHip c) Left: Representative images validate GCaMP6s expression in vHip. Scale bar = 500 µm. Right: Representative images showing the overlap between GCaMP6s‐expressing cells (green) and glutamatergic neurons (violet). Scale bar = 50 µm. d) Representative images of c‐Fos‐positive glutamatergic neurons. Scale bars = 100 or 20 µm. e) Statistical analysis of c‐Fos co‐labeled glutamatergic neuron in vHip of mice. f) Schematic of fiber photometry recording in TST and representative Ca 2+ signals photometric traces in response to the TST during struggling and immobility phases. Scale bars = 10 s. g) Ca 2 ⁺ signal recording in vHip during TST. Left: Representative heatmaps showing Ca 2 ⁺ signal in response to TST during struggling. Right: Z‐score traces and quantification of average Z‐scores during TST. n = 5 mice per group. h) Representative images of the AAV vectors engineered to express the glutamate sensor iGluSnFR under the hSyn promoter in the vHip. Scale bars = 500 µm / 50 µm. i) Representative trace of glutamate signal event detection during TST. Red dots indicate detected events. j) Event‐based quantification. Event frequency (left), AUC (middle) and decay tau (right). n = 5 mice per group. k) Timeline of electrophysiological recordings from vHip glutamatergic neurons. l) Quantification of mEPSC frequency (left) and amplitude (right) recorded from vHip glutamatergic neurons. n = 10–12 cells from 3 mice per group. m) Left: Evoked firing rates of action potentials recorded from eGFP⁺ neurons in the vHip. Right: Quantifications of rheobase and resting membrane potential recorded from eGFP + neurons in vHip. n = 10 cells from 3 mice per group. Statistical analyses included unpaired t‐test, one‐way/two‐way ANOVA followed by Tukey's post hoc test and Kruskal‐Wallis test. Data are presented as means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Journal: Advanced Science

Article Title: Astrocytic PCBP1 Suppresses Ferroptosis to Restore Glutamatergic Homeostasis and Mitigate Stress‐Induced Depression in Male Mice

doi: 10.1002/advs.202513438

Figure Lengend Snippet: Astrocytic PCBP1 enhances glutamatergic neuronal activity and neurophysiology. a) c‐Fos expression in vHip of mice following CUMS. Above: Representative images and quantification of c‐Fos‐positive glutamatergic neurons. Below: Representative images and quantification of c‐Fos‐positive GABAergic neurons. Scale bars = 50 µm. n = 9 slices from 3 animals per group. b) Timeline of fiber photometry recording Ca 2+ and glutamate signals from vHip c) Left: Representative images validate GCaMP6s expression in vHip. Scale bar = 500 µm. Right: Representative images showing the overlap between GCaMP6s‐expressing cells (green) and glutamatergic neurons (violet). Scale bar = 50 µm. d) Representative images of c‐Fos‐positive glutamatergic neurons. Scale bars = 100 or 20 µm. e) Statistical analysis of c‐Fos co‐labeled glutamatergic neuron in vHip of mice. f) Schematic of fiber photometry recording in TST and representative Ca 2+ signals photometric traces in response to the TST during struggling and immobility phases. Scale bars = 10 s. g) Ca 2 ⁺ signal recording in vHip during TST. Left: Representative heatmaps showing Ca 2 ⁺ signal in response to TST during struggling. Right: Z‐score traces and quantification of average Z‐scores during TST. n = 5 mice per group. h) Representative images of the AAV vectors engineered to express the glutamate sensor iGluSnFR under the hSyn promoter in the vHip. Scale bars = 500 µm / 50 µm. i) Representative trace of glutamate signal event detection during TST. Red dots indicate detected events. j) Event‐based quantification. Event frequency (left), AUC (middle) and decay tau (right). n = 5 mice per group. k) Timeline of electrophysiological recordings from vHip glutamatergic neurons. l) Quantification of mEPSC frequency (left) and amplitude (right) recorded from vHip glutamatergic neurons. n = 10–12 cells from 3 mice per group. m) Left: Evoked firing rates of action potentials recorded from eGFP⁺ neurons in the vHip. Right: Quantifications of rheobase and resting membrane potential recorded from eGFP + neurons in vHip. n = 10 cells from 3 mice per group. Statistical analyses included unpaired t‐test, one‐way/two‐way ANOVA followed by Tukey's post hoc test and Kruskal‐Wallis test. Data are presented as means ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: For immunofluorescence analysis, the 30‐μm‐thick sections were incubated overnight at 4 °C with primary antibodies against NEUN (94403S, Cell Signaling Technology), GFAP (GB11096, Servicebio), IBA‐1 (GB12105, Servicebio), CaMKII (11533‐1‐AP, Proteintech), GAD67 (PA5‐21397, Invitrogen), c‐Fos (OB‐PGP080, Oasis biogarm), GPX4 (ab125066, Abcam), and PCBP1 (14523‐1‐AP, Proteintech).

Techniques: Activity Assay, Expressing, Labeling, Membrane

A, AP1 DNA-binding activity of 25 microg of nuclear proteins. Nuclear extracts from NFRKB-transfected HEK cells were incubated with the wild-type AP1 oligonucleotide (Wt AP1) or with mutant-type AP1(Mut AP1) oligonucleotide. B, Expression of c-fos and c-jun. Immunoblots were performed with 50 microg of proteins. C, HEK cells were transiently co-transfected with the NFRKB or empty vector (Ev), and AP1-Luc reporter plasmid. The luciferase activity was measured using the “Dual Luciferase reporter Assay”. The data are presented as relative luciferase activity (firefly luciferase/the renilla luciferase). Statistical analyses were carried out on data from five independent experiments using the one-way Anova (** p <0.015; Kruskal-Wallis test).

Journal: PLoS ONE

Article Title: Upregulation of Nuclear Factor-Related Kappa B Suggests a Disorder of Transcriptional Regulation in Minimal Change Nephrotic Syndrome

doi: 10.1371/journal.pone.0030523

Figure Lengend Snippet: A, AP1 DNA-binding activity of 25 microg of nuclear proteins. Nuclear extracts from NFRKB-transfected HEK cells were incubated with the wild-type AP1 oligonucleotide (Wt AP1) or with mutant-type AP1(Mut AP1) oligonucleotide. B, Expression of c-fos and c-jun. Immunoblots were performed with 50 microg of proteins. C, HEK cells were transiently co-transfected with the NFRKB or empty vector (Ev), and AP1-Luc reporter plasmid. The luciferase activity was measured using the “Dual Luciferase reporter Assay”. The data are presented as relative luciferase activity (firefly luciferase/the renilla luciferase). Statistical analyses were carried out on data from five independent experiments using the one-way Anova (** p <0.015; Kruskal-Wallis test).

Article Snippet: The double-stranded oligonucleotide probes (100 ng), with the consensus and mutant NF-kB (sc-2505, sc-2511), GAS/ISRE (sc-2537, sc-2538), NFATc (sc-2577, sc-2578) and AP1 (sc-2501, sc2514) sequences, respectively, were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

Techniques: Binding Assay, Activity Assay, Transfection, Incubation, Mutagenesis, Expressing, Western Blot, Plasmid Preparation, Luciferase, Reporter Assay