Journal: Human Reproduction (Oxford, England)

Article Title: Endometriosis expresses a molecular pattern consistent with decreased retinoid uptake, metabolism and action

doi: 10.1093/humrep/der172

Figure Lengend Snippet: There is a differential gene expression of multiple genes involved in the retinoic acid signaling cascade in normal endometrium when compared with endometriotic tissues.

Article Snippet: Equal amounts of protein (40 µg for tissue, 20 µg for stromal cells, 10 µg for nuclear extracts) were resolved on 4–20% Ready Gel Precast Gels (Bio-Rad Laboratories, Hercules, CA, USA), for 90 min at 60 mAmp and transferred to a 0.45 mm pore nitrocellulose membranes at 150 mAmps for 2 h. The membranes were blotted for a minimum of 1 h in a solution of 1% dried milk in Tris-buffered saline at room temperature and incubated with the primary antibodies at 4°C overnight (STRA6 antibody graciously donated; CRBP1, ALDH1A2, CRABP2, FABP5, RARα, RXRα, CYP26B1 and PPARβ/δ antibodies from Abcam, Cambridge, MA, USA).

Techniques: Expressing, Binding Assay