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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Cardioprotective Effects of the GRK2 Inhibitor Paroxetine on Isoproterenol-Induced Cardiac Remodeling by Modulating NF-κB Mediated Prohypertrophic and Profibrotic Gene Expression
doi: 10.3390/ijms242417270
Figure Lengend Snippet: Effect of paroxetine on ISO-induced NF-κB and inflammatory biomarkers. ( A ) Level of NFκB(p105) in myocardium tissues increased with ISO treatment (CH group) and was prevented by paroxetine pre-treatment (CH + P group) ( n = 6 per group). Serum levels of inflammatory biomarkers IL6 ( n = 4) ( B ) and CRP ( n = 6) ( C ) increased with ISO treatment (CH group) and were prevented by paroxetine and fluoxetine pre-treatment (CH + P and CH + F groups). All data are expressed as mean ± SEM. Differences between groups were detected using one-way ANOVA followed by Dunnett’s post hoc test. Statistically significant differences between groups are shown as * p < 0.05, *** p < 0.001. CH: cardiac hypertrophy untreated; CH + P: cardiac hypertrophy pretreated with paroxetine; CH + F: cardiac hypertrophy pretreated with fluoxetine; NFκB: Nuclear factor-kappa-light-chain-enhancer of activated B-cells; IL6: interleukin 6; CRP: C-reactive protein.
Article Snippet: Paraffin-embedded heart tissue sections (5 µm) were deparaffinized and then incubated with the following primary antibodies: anti-GRK2 (1:50) (Santa Cruz Biotechnology, Santa Cruz, CA, USA), anti-IκBα(pSer32/S36) (1:100), and anti-NFκB(
Techniques: