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Image Search Results
Journal: PLoS ONE
Article Title: Cyp1b1 expression impacts the angiogenic and inflammatory properties of liver sinusoidal endothelial cells
doi: 10.1371/journal.pone.0206756
Figure Lengend Snippet: The expression of ICAM-1, VCAM-1, CD36, and CD47 were determined by FACS analysis of Cyp1b1 +/+ and Cyp1b1-/- LSEC. Please note a dramatic decrease in the level of ICAM-1 in Cyp1b1-/- LSEC as compared with wild type cells. Levels of ICAM-2, VCAM-1 and CD47 remained similar between the two cell types. Expression of CD36 was modestly increased Cyp1b1-/- LSEC. These experiments were repeated with two isolations of LSEC with similar results.
Article Snippet: The following primary antibodies were used: anti-PECAM-1 (553370; BD Biosciences), anti-VE-cadherin (ALX-210-232-C100; Enzo Life Sciences), B4-lectin (FL1201,Vector Labs),
Techniques: Expressing
Journal: Nature Communications
Article Title: Pericytes regulate VEGF-induced endothelial sprouting through VEGFR1
doi: 10.1038/s41467-017-01738-3
Figure Lengend Snippet: Pericytes control endothelial behavior in angiogenesis. a Confocal images showing proliferating cells (EdU, blue), IB4 (red) and EC nuclei (Erg1, green) in the P6 control and DTR iPC retinal angiogenic front, as indicated. Images on the right show higher magnification of boxed insets in left column. Dashed lines outline the angiogenic leading vessel from where the sprouts emerge (second column) or vessel shape (fourth column) at the edge of the vascular plexus. Scale bar, 100 µm (left panels) and 50 µm (higher magnifications). b – d Quantitation of total b and regional EC proliferation c as well as EC density d within the peripheral vascular plexus of P6 control and DTR iPC retinas. Error bars, s.e.m. p -values, Student’s t -test. NS, not statistically significant. e High magnification of IB4 (red) and Erg1 (green) stained sprouts highlighting the accumulation of EC nuclei in P6 DTR iPC but not control sprouts. Scale bar, 50 µm. f Confocal images showing IB4 (red), NG2+ pericytes (green) and vessel lumen (ICAM2, white) in the control and DTR iPC angiogenic front of P6 retinas. Images on the right show higher magnification of boxed insets in the second column. Note that IB4 signal covers a larger area than the apical ICAM2 immunostaining (arrowheads in third column). Scale bar, 100 µm (left panels) and 50 µm (higher magnifications)
Article Snippet: Moreover, the following primary antibodies were used: rabbit anti-collagen type IV (colIV, 1:100, Millipore, AB756P), rat monoclonal anti-laminin α5 (LAMA5, 1:50, clone 4G A2 11, kindly provided by Prof. Dr. Lydia Sorokin, Institute of Physiological Chemistry and Pathobiochemistry, University of Münster, Germany), goat anti-PDGFRβ (1:100, R&D, AF1042), rat anti-PDGFRβ (1:100, eBioscience, 14–1402), rabbit anti-desmin (1:100, Abcam, ab15200), rabbit anti-NG2 (1:200, Millipore, AB5320), rabbit anti-Erg1 (1:100, Abcam, ab110639),
Techniques: Quantitation Assay, Staining, Immunostaining