antigen-antibody%20complexes Search Results


95
Miltenyi Biotec cd3 pe vio770
Cd3 Pe Vio770, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Alomone Labs rabbit anti eag1
Rabbit Anti Eag1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec t cells
T Cells, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Proteintech ncapg
Ncapg, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec anti mouse cd3 mab
Anti Mouse Cd3 Mab, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec anti biotin antibody
Anti Biotin Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio anti cd74
Anti Cd74, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Boster Bio anti cyp1a2 antibodies
Anti Cyp1a2 Antibodies, supplied by Boster Bio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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StressMarq anti e cadherin
Anti E Cadherin, supplied by StressMarq, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec reafinity

Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec cd3 percp vio700
The autotaxin-NFκB-Cxcl1 axis impairs TGFβ inhibition by recruiting MDSCs while inhibiting cytotoxic CD8 + T-cell infiltration. A, Histogram shows Cxcl1 plasmatic concentration in mice inoculated with RC416 cells and treated as indicated. Means and SD ( n = 5) are shown. P value was calculated by ANOVA and Tukey test. B, qPCR of Cxcl1 in RC416, FC1199, and B6KPC cells as single cultures or in coculture with PSCs. Data are represented as mean ± SD ( n = 3). P value was calculated by ANOVA and Tukey test. C, Schematic representation of Cxcl1 promoter (available at UCSC Genome Browser GRCm38/mm10, chr5:90887842-90889342) with the position of ChIP probes (gray double arrowhead) and consensus NFκB binding sites (vertical slashes) relative to the transcription start site (TSS). D, ChIP-qPCR of NFκB occupancy at the Cxcl1 promoter. The y -axis represents relative promoter enrichment, normalized on input material. IgG was set to 1. Data are represented as mean ± SD ( n = 3). E, Flow cytometry analysis of CD45 + /CD11b + /Ly6C + /Ly6G − monocytic (M)-MDSCs and CD45 + /CD11b + /LY6g + /LY6c low granulocytic (G)-MDSCs in RC416 tumor samples treated as indicated. F, Flow cytometry analysis of CTLs (CD45 + <t>/CD3</t> + /CD8 + /CD178(FasL) + ) in RC416 tumor samples treated as indicated. Data in E and F are represented as mean ± SD ( n = 4). G and H, Quantification ( G ) and representative images ( H ) of CD8 + T lymphocytes in paraffin sections from RC416 tumors treated as indicated. Scale bar, 150 μm. P values in A, B, D, E, and F were calculated by ANOVA and Tukey test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.
Cd3 Percp Vio700, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec fitc anti cd3
The autotaxin-NFκB-Cxcl1 axis impairs TGFβ inhibition by recruiting MDSCs while inhibiting cytotoxic CD8 + T-cell infiltration. A, Histogram shows Cxcl1 plasmatic concentration in mice inoculated with RC416 cells and treated as indicated. Means and SD ( n = 5) are shown. P value was calculated by ANOVA and Tukey test. B, qPCR of Cxcl1 in RC416, FC1199, and B6KPC cells as single cultures or in coculture with PSCs. Data are represented as mean ± SD ( n = 3). P value was calculated by ANOVA and Tukey test. C, Schematic representation of Cxcl1 promoter (available at UCSC Genome Browser GRCm38/mm10, chr5:90887842-90889342) with the position of ChIP probes (gray double arrowhead) and consensus NFκB binding sites (vertical slashes) relative to the transcription start site (TSS). D, ChIP-qPCR of NFκB occupancy at the Cxcl1 promoter. The y -axis represents relative promoter enrichment, normalized on input material. IgG was set to 1. Data are represented as mean ± SD ( n = 3). E, Flow cytometry analysis of CD45 + /CD11b + /Ly6C + /Ly6G − monocytic (M)-MDSCs and CD45 + /CD11b + /LY6g + /LY6c low granulocytic (G)-MDSCs in RC416 tumor samples treated as indicated. F, Flow cytometry analysis of CTLs (CD45 + <t>/CD3</t> + /CD8 + /CD178(FasL) + ) in RC416 tumor samples treated as indicated. Data in E and F are represented as mean ± SD ( n = 4). G and H, Quantification ( G ) and representative images ( H ) of CD8 + T lymphocytes in paraffin sections from RC416 tumors treated as indicated. Scale bar, 150 μm. P values in A, B, D, E, and F were calculated by ANOVA and Tukey test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.
Fitc Anti Cd3, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Journal: Cancer Cell

Article Title: Retinoic acid receptor activation reprograms senescence response and enhances anti-tumor activity of natural killer cells

doi: 10.1016/j.ccell.2024.02.004

Figure Lengend Snippet:

Article Snippet: CD3 Antibody, anti-mouse, APC-Vio770, REAfinity , Miltenyi Biotec , Cat# 130-119-793; RRID: AB_2751847.

Techniques: Control, Purification, Recombinant, Staining, Reverse Transcription, Cell Isolation, Isolation, cDNA Synthesis, Gene Expression, shRNA, Plasmid Preparation, CRISPR, Software, Transfection, In Vitro, Imaging

The autotaxin-NFκB-Cxcl1 axis impairs TGFβ inhibition by recruiting MDSCs while inhibiting cytotoxic CD8 + T-cell infiltration. A, Histogram shows Cxcl1 plasmatic concentration in mice inoculated with RC416 cells and treated as indicated. Means and SD ( n = 5) are shown. P value was calculated by ANOVA and Tukey test. B, qPCR of Cxcl1 in RC416, FC1199, and B6KPC cells as single cultures or in coculture with PSCs. Data are represented as mean ± SD ( n = 3). P value was calculated by ANOVA and Tukey test. C, Schematic representation of Cxcl1 promoter (available at UCSC Genome Browser GRCm38/mm10, chr5:90887842-90889342) with the position of ChIP probes (gray double arrowhead) and consensus NFκB binding sites (vertical slashes) relative to the transcription start site (TSS). D, ChIP-qPCR of NFκB occupancy at the Cxcl1 promoter. The y -axis represents relative promoter enrichment, normalized on input material. IgG was set to 1. Data are represented as mean ± SD ( n = 3). E, Flow cytometry analysis of CD45 + /CD11b + /Ly6C + /Ly6G − monocytic (M)-MDSCs and CD45 + /CD11b + /LY6g + /LY6c low granulocytic (G)-MDSCs in RC416 tumor samples treated as indicated. F, Flow cytometry analysis of CTLs (CD45 + /CD3 + /CD8 + /CD178(FasL) + ) in RC416 tumor samples treated as indicated. Data in E and F are represented as mean ± SD ( n = 4). G and H, Quantification ( G ) and representative images ( H ) of CD8 + T lymphocytes in paraffin sections from RC416 tumors treated as indicated. Scale bar, 150 μm. P values in A, B, D, E, and F were calculated by ANOVA and Tukey test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Journal: Cancer Research

Article Title: Autotaxin Secretion Is a Stromal Mechanism of Adaptive Resistance to TGFβ Inhibition in Pancreatic Ductal Adenocarcinoma

doi: 10.1158/0008-5472.CAN-23-0104

Figure Lengend Snippet: The autotaxin-NFκB-Cxcl1 axis impairs TGFβ inhibition by recruiting MDSCs while inhibiting cytotoxic CD8 + T-cell infiltration. A, Histogram shows Cxcl1 plasmatic concentration in mice inoculated with RC416 cells and treated as indicated. Means and SD ( n = 5) are shown. P value was calculated by ANOVA and Tukey test. B, qPCR of Cxcl1 in RC416, FC1199, and B6KPC cells as single cultures or in coculture with PSCs. Data are represented as mean ± SD ( n = 3). P value was calculated by ANOVA and Tukey test. C, Schematic representation of Cxcl1 promoter (available at UCSC Genome Browser GRCm38/mm10, chr5:90887842-90889342) with the position of ChIP probes (gray double arrowhead) and consensus NFκB binding sites (vertical slashes) relative to the transcription start site (TSS). D, ChIP-qPCR of NFκB occupancy at the Cxcl1 promoter. The y -axis represents relative promoter enrichment, normalized on input material. IgG was set to 1. Data are represented as mean ± SD ( n = 3). E, Flow cytometry analysis of CD45 + /CD11b + /Ly6C + /Ly6G − monocytic (M)-MDSCs and CD45 + /CD11b + /LY6g + /LY6c low granulocytic (G)-MDSCs in RC416 tumor samples treated as indicated. F, Flow cytometry analysis of CTLs (CD45 + /CD3 + /CD8 + /CD178(FasL) + ) in RC416 tumor samples treated as indicated. Data in E and F are represented as mean ± SD ( n = 4). G and H, Quantification ( G ) and representative images ( H ) of CD8 + T lymphocytes in paraffin sections from RC416 tumors treated as indicated. Scale bar, 150 μm. P values in A, B, D, E, and F were calculated by ANOVA and Tukey test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.

Article Snippet: MDSCs and CD8 phenotype were evaluated with the following antibodies: CD45-APC-Vio770 (REA737), CD11b-APC (REA592), Ly6c-FITC (REA796), Ly6g-PE-Vio770 (REA526), CD3-PerCP-Vio700 (REA641), CD8-Vioblue (REAL187), CD178 Pe-Vio615 (REA1171) and the Fixable die Viobility 405/520, all from Miltenyi Biotec.

Techniques: Inhibition, Concentration Assay, Binding Assay, ChIP-qPCR, Flow Cytometry