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Image Search Results
Journal: Cancer Cell
Article Title: Retinoic acid receptor activation reprograms senescence response and enhances anti-tumor activity of natural killer cells
doi: 10.1016/j.ccell.2024.02.004
Figure Lengend Snippet:
Article Snippet: CD3 Antibody, anti-mouse, APC-Vio770,
Techniques: Control, Purification, Recombinant, Staining, Reverse Transcription, Cell Isolation, Isolation, cDNA Synthesis, Gene Expression, shRNA, Plasmid Preparation, CRISPR, Software, Transfection, In Vitro, Imaging
Journal: Cancer Research
Article Title: Autotaxin Secretion Is a Stromal Mechanism of Adaptive Resistance to TGFβ Inhibition in Pancreatic Ductal Adenocarcinoma
doi: 10.1158/0008-5472.CAN-23-0104
Figure Lengend Snippet: The autotaxin-NFκB-Cxcl1 axis impairs TGFβ inhibition by recruiting MDSCs while inhibiting cytotoxic CD8 + T-cell infiltration. A, Histogram shows Cxcl1 plasmatic concentration in mice inoculated with RC416 cells and treated as indicated. Means and SD ( n = 5) are shown. P value was calculated by ANOVA and Tukey test. B, qPCR of Cxcl1 in RC416, FC1199, and B6KPC cells as single cultures or in coculture with PSCs. Data are represented as mean ± SD ( n = 3). P value was calculated by ANOVA and Tukey test. C, Schematic representation of Cxcl1 promoter (available at UCSC Genome Browser GRCm38/mm10, chr5:90887842-90889342) with the position of ChIP probes (gray double arrowhead) and consensus NFκB binding sites (vertical slashes) relative to the transcription start site (TSS). D, ChIP-qPCR of NFκB occupancy at the Cxcl1 promoter. The y -axis represents relative promoter enrichment, normalized on input material. IgG was set to 1. Data are represented as mean ± SD ( n = 3). E, Flow cytometry analysis of CD45 + /CD11b + /Ly6C + /Ly6G − monocytic (M)-MDSCs and CD45 + /CD11b + /LY6g + /LY6c low granulocytic (G)-MDSCs in RC416 tumor samples treated as indicated. F, Flow cytometry analysis of CTLs (CD45 + /CD3 + /CD8 + /CD178(FasL) + ) in RC416 tumor samples treated as indicated. Data in E and F are represented as mean ± SD ( n = 4). G and H, Quantification ( G ) and representative images ( H ) of CD8 + T lymphocytes in paraffin sections from RC416 tumors treated as indicated. Scale bar, 150 μm. P values in A, B, D, E, and F were calculated by ANOVA and Tukey test. *, P < 0.05; **, P < 0.01; ***, P < 0.001.
Article Snippet: MDSCs and CD8 phenotype were evaluated with the following antibodies: CD45-APC-Vio770 (REA737), CD11b-APC (REA592), Ly6c-FITC (REA796), Ly6g-PE-Vio770 (REA526),
Techniques: Inhibition, Concentration Assay, Binding Assay, ChIP-qPCR, Flow Cytometry