Journal: Gastric Cancer

Article Title: Bone marrow derived “fibrocytes” contribute to tumor proliferation and fibrosis in gastric cancer

doi: 10.1007/s10120-014-0380-0

Figure Lengend Snippet: Preparation of fibrocytes. Crude fibrocyte preparations derived from PBMCs were depleted of contaminating T cells, B cells, and monocytes by immunomagnetic negative selection. The cells were stained with monoclonal mouse-anti human CD45 conjugated to phycoerythrin (PE) and monoclonal anti-human COLA-IA conjugated to fluorescein isothiocyanate (FITC) and analyzed by flow cytometry

Article Snippet: To determine the purity of these fibrocyte preparations, the cells (5 × 10 5 /100 μL) were incubated in the dark for 30 min at 4 °C with a monoclonal mouse-anti human CD45 antibody conjugated to phycoerythrin (PE; BD Biosciences, San Diego, CA, USA) and a mouse anti-collagen type I antibody conjugated to fluorescein isothiocyanate (FITC; Rockland, Inc., Gilbertsville, PA, USA).

Techniques: Derivative Assay, Selection, Staining, Flow Cytometry