Journal: Cell Death & Disease

Article Title: Oncolytic peptide LTX-315 plus an anti-CTLA-4 antibody induces a synergistic anti-cancer immune response in residual tumors after radiofrequency ablation of hepatocellular carcinoma

doi: 10.1038/s41419-025-07622-z

Figure Lengend Snippet: A Experimental workflow for silencing the cGAS-STING pathway and ICD in vivo studies. B , C Western blotting was used to confirm the knockout of cGAS and STING proteins in Hepa1-6 cells. D , E The proportion of mature dendritic cells (CD80 + CD86 + DCs) was assessed after treatment with the anti-CD11c antibody. F , G Western blotting was applied to confirm the reduction of ICD-related molecules (ATP, CALR, HMGB1) after treatment with the anti-CD11c antibody. H , I Phosphorylation of TBK1 and IRF3 was reduced in tumors after silencing the cGAS-STING pathway. J , K Tumors were harvested and weight was assessed, showing an increase in tumor size after silencing the cGAS-STING pathway and ICD in all five groups. L – N Flow cytometry analysis was used to evaluate the levels of functional CD8 + T cells after silencing the cGAS-STING pathway and ICD in all five groups. n = 6 per group. Error bars represent standard deviation. Ns not statistically significant. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: CD11c monoclonal antibodies (BioXCell, NH, USA) were administered intraperitoneally at 100 μg/mouse, starting 7 days before combination therapy and repeated weekly for 3 weeks.

Techniques: In Vivo, Western Blot, Knock-Out, Phospho-proteomics, Flow Cytometry, Functional Assay, Standard Deviation

Journal: Frontiers in Nutrition

Article Title: High intake of n-6 polyunsaturated fatty acid exacerbates non-alcoholic steatohepatitis by the involvement of multiple metabolic pathways

doi: 10.3389/fnut.2025.1562509

Figure Lengend Snippet: Effects of n-6 PUFA on liver macrophage phenotype in rats with NASH induced by a choline-deficient diet. (A) M1-type Kupffer cells (KCs) identified by double staining: red arrows show CD11c-positive cells, green arrows show CD68-positive cells, and yellow arrows highlight CD11c and CD68 double-positive M1-type KCs (Scale bar – 50 μM). (B) M2-type KCs identified similarly, with red arrows indicating CD163-positive cells, green arrows showing CD68-positive cells, and yellow arrows marking CD163 and CD68 double-positive M2-type KCs (Scale bar – 50 μM). For (A,B) (see ) for full-size photomicrographs. (C) M1/M2 phenotype ratio (unitless), calculated as the proportion of CD68 + CD11c + to CD68 + CD163 + cells. (D) Relative PPAR-γ2 mRNA expression (fold change normalized to GAPDH) in the liver, which is linked to macrophage polarization and inflammation. Data are expressed as mean ±SEM; n = 6/group.

Article Snippet: BCA protein quantitation kit (Boster Bio, Cat # AR0146), RIPA lysis buffer (Boster Bio, Cat # 0105), protease inhibitor cocktails (Boster, Bio Cat # AR1182), phosphatase inhibitor (Boster Bio, Cat # AR1183), color pre-dyed protein marker (Boster Bio, Cat # AR1113), Western-specific primary and secondary antibody diluent (Boster Bio, Cat # AR1017), wash buffer TBS-T (Boster Bio, Cat # AR0195-10), ECL chemiluminescent reagent (Boster Bio, Cat # AR1196), BSA TBS buffer system blocking solution (Boster Bio, Cat # AR0189), NF-κB antibody (Boster Bio, Cat # A01228-1), GAPDH antibody (Boster Bio, Cat # M00227), HRP-conjugated goat anti-rabbit IgG (Boster Bio, Cat # BA1054), CD163 antibody (Boster Bio, Cat # A00812-2), CD11c antibody (Boster Bio, Cat # A00357-3), CD68 antibody (Boster Bio, Cat # BA3638), fluorescent (DyLight 488) labeled goat anti-rabbit IgG (Boster Bio, Cat # BA1127), fluorescent (DyLight 594) labeled goat anti-rabbit IgG (Boster Bio, Cat # BA1142), rat TNF-α ELISA kit (Jiangsu Enzyme Immunoassay Co., Ltd., Cat # MM-0180R1), rat IL-1β ELISA kit (Boster Bio, Cat # EK0393), rat IL-4 ELISA kit (Jiangsu Enzyme Immunoassay Co., Ltd., Cat # MM-0191R1), rat IL-10 ELISA kit (Boster Bio, Cat # EK0418), 25 VD3 assay kit (Roche Diagnostics, Cat # 07028148190), TAOC assay kit (Nanjing Jiancheng, Cat # A015-3-1), MDA assay kit (Nanjing Jiancheng, Cat # A003-1-2), and free fatty acid kit (Kunchuang Biotechnology, Xian, China, Cat # SK125-2).

Techniques: Double Staining, Expressing

Journal: iScience

Article Title: Temporal and spatial dynamics of immune cells in spontaneous liver transplant tolerance

doi: 10.1016/j.isci.2023.107691

Figure Lengend Snippet: List of metal-labeled antibodies used for mass cytometry

Article Snippet: Anti-CD11c , Fluidigm , 3142003C.

Techniques:

Journal: iScience

Article Title: Temporal and spatial dynamics of immune cells in spontaneous liver transplant tolerance

doi: 10.1016/j.isci.2023.107691

Figure Lengend Snippet: Immunohistochemical staining of different cell markers in hepatic grafts Different immune cell markers (B220, CD3, CD8, Ly6G, I-A k , CD11c, and F4/80) were stained in hepatic grafts in Naïve control (Naïve), syngeneic control (Syn), postoperative days (POD) 7, POD14, POD30, and POD100 groups. Image data are representative of three independent experiments. Scale bar = 200 μm.

Article Snippet: Anti-CD11c , Fluidigm , 3142003C.

Techniques: Immunohistochemistry, Staining

Journal: iScience

Article Title: Temporal and spatial dynamics of immune cells in spontaneous liver transplant tolerance

doi: 10.1016/j.isci.2023.107691

Figure Lengend Snippet:

Article Snippet: Anti-CD11c , Fluidigm , 3142003C.

Techniques: Transplantation Assay, Recombinant, Staining, Cell Isolation, Software, Cytometry, Flow Cytometry

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy

doi:

Figure Lengend Snippet: Oligonucleotide primers of CD11, CD11b, CD11c, and β-actin

Article Snippet: Primary antibodies of CD11a, CD11b, and CD11c (1:100, Wuhan Boster Company) were incubated overnight at 4°C and then incubated at 37°C for 30 min in IgG/Biotin IgG and SABC liquid.

Techniques:

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy

doi:

Figure Lengend Snippet: RT-PCR products of CD11a, CD11b, and CD11c A: RT-PCR products of CD11a M: Marker 1-2: experimental group; 3-4: control group B: RT-PCR products of CD11b M: Marker 1-2: control group; 3-4: experimental group C: RT-PCR products of CD11c M: Marker 1-2: experimental group; 3-4: control group

Article Snippet: Primary antibodies of CD11a, CD11b, and CD11c (1:100, Wuhan Boster Company) were incubated overnight at 4°C and then incubated at 37°C for 30 min in IgG/Biotin IgG and SABC liquid.

Techniques: Reverse Transcription Polymerase Chain Reaction, Marker, Control

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy

doi:

Figure Lengend Snippet: Comparison of the average area and brightness of CD11a, CD11b, and CD11c expressions in the myocardium ( x ¯ ±s)

Article Snippet: Primary antibodies of CD11a, CD11b, and CD11c (1:100, Wuhan Boster Company) were incubated overnight at 4°C and then incubated at 37°C for 30 min in IgG/Biotin IgG and SABC liquid.

Techniques: Comparison, Control

Journal: Iranian Journal of Basic Medical Sciences

Article Title: Expressions of CD11a, CD11b, and CD11c integrin proteins in rats with myocardial hypertrophy

doi:

Figure Lengend Snippet: Expressions of CD11a, CD11b, and CD11c in the myocardial tissue at mRNA levels A: CD11a of control group B: CD11b of control group C: CD11c of control group D: CD11a of experimental group E: CD11b of experimental group F: CD11c of experimental group Arrows was used to indicate the positive expression of integrins in experimental group.

Article Snippet: Primary antibodies of CD11a, CD11b, and CD11c (1:100, Wuhan Boster Company) were incubated overnight at 4°C and then incubated at 37°C for 30 min in IgG/Biotin IgG and SABC liquid.

Techniques: Control, Expressing

Journal: PLoS ONE

Article Title: Cathepsin L Inhibition Prevents Murine Autoimmune Diabetes via Suppression of CD8 + T Cell Activity

doi: 10.1371/journal.pone.0012894

Figure Lengend Snippet: (A) Flow cytometric analysis was performed to detect intracellular Foxp3 in CD4 + T cells of pancreatic lymph nodes (PLNs) from control mice, CY-treated mice and CY+CatL-inh-treated mice. (B, C) CD44 expressions on CD4 + and CD8 + T cells in PLNs from control, CY-treated, and CY+CatL-inh-treated mice were analyzed by flow cytometry. Results are representative of 3 to 5 mice in each group. (D) Enzymatic activities of cathepsin L in thymus and PLNs from control and CY-treated NOD mice. (E) The mRNA expressions of cathepsin L were detected by real-time PCR using purified CD8 + , CD4 + , CD4 + CD25 + , B220 + and CD11c + cells of PLNs from NOD mice. Data are shown as means ± s.d. of 3 to 4 mice in each group.

Article Snippet: CD8 + cells, CD4 + cells, CD4 + CD25 + cells, B220 + cells and CD11c + cells were prepared from PLNs by positive selection using anti-CD8, CD4, CD25, B220 (eBioscience) and CD11c (MBL International, Woburn, MA) antibodies, and magnetic beads (Dynal Biotech, Oslo, Norway).

Techniques: Control, Flow Cytometry, Real-time Polymerase Chain Reaction, Purification

Journal: Mediators of Inflammation

Article Title: The Acute Phase of Trypanosoma cruzi Infection Is Attenuated in 5-Lipoxygenase-Deficient Mice

doi: 10.1155/2014/893634

Figure Lengend Snippet: Quantitative and functional leukocyte responses to T. cruzi infection. (a) Gr1 + cell (neutrophils); (b) Gr1 + CD11c + cell (pDC cells); (c) CD11b + cell (myeloid lineage cell marker, Mac-1); and (d) F4/80 + cell (macrophages) numbers in the spleen.

Article Snippet: The following fluorochrome-conjugated monoclonal antibodies were used: anti-CD4 [H129.19]; anti-CD8 [53-6.7]; anti-CD19 [MB19-1]; anti-CD25 [7D4]; anti-CD44 [IM7]; anti-CD69 [H1.2F3]; anti-Gr-1/Ly6C/Ly6G [RB6-8C5]; anti-CD45RB [16A]; anti-CD62L [MEL-14]; anti-CD11b [M1/70] (BD Pharmingen, San Diego, CA); and anti-CD11c [HL3]—(Serotech, Raleigh, NC) anti-F4/80 [CI:A3-1] and anti-GITR [DTA-1] (eBioscience, San Diego, CA).

Techniques: Functional Assay, Infection, Marker