Journal: Biomarker Insights

Article Title: mRNA Expression and Methylation of the RAD51 , ATM , ATR , BRCA1 , and BRCA2 Genes in Gastric Adenocarcinoma

doi: 10.1177/11772719231225206

Figure Lengend Snippet: RAD51 , ATR , BRCA1 , BRCA2 , and ATM mRNA expression in gastric adenocarcinoma and non-tumor gastric mucosa samples.

Article Snippet: The following antibodies were used: ATM (St John’s Laboratory, London, UK, STJ97797, 1:400), ATR (Abcam, Cambridge, UK, ab178407, 1:100), BRCA1 (St John’s Laboratory, STJ113833, 1:300), RAD51 (St John’s Laboratory, STJ95330, 1:100), and BRCA2 (St John’s Laboratory, STJ91885, 1:100).

Techniques: Expressing

Journal: Biomarker Insights

Article Title: mRNA Expression and Methylation of the RAD51 , ATM , ATR , BRCA1 , and BRCA2 Genes in Gastric Adenocarcinoma

doi: 10.1177/11772719231225206

Figure Lengend Snippet: Comparison of RAD51 , ATR , BRCA1 , BRCA2 , and ATM mRNA expression in tumor and non-tumor mucosa, considering the paired samples of the same individuals. The results are presented in box plots, using a logarithmic scale (+1 was added as a constant to allow the logarithmic representation of null values).

Article Snippet: The following antibodies were used: ATM (St John’s Laboratory, London, UK, STJ97797, 1:400), ATR (Abcam, Cambridge, UK, ab178407, 1:100), BRCA1 (St John’s Laboratory, STJ113833, 1:300), RAD51 (St John’s Laboratory, STJ95330, 1:100), and BRCA2 (St John’s Laboratory, STJ91885, 1:100).

Techniques: Comparison, Expressing

Journal: Biomarker Insights

Article Title: mRNA Expression and Methylation of the RAD51 , ATM , ATR , BRCA1 , and BRCA2 Genes in Gastric Adenocarcinoma

doi: 10.1177/11772719231225206

Figure Lengend Snippet: Comparison between RAD51 , ATR , BRCA1 , BRCA2 , and ATM mRNA expression in patients with previous neoadjuvant chemotherapy and in those without neoadjuvant chemotherapy, considering tumor samples and non-tumor mucosa samples.

Article Snippet: The following antibodies were used: ATM (St John’s Laboratory, London, UK, STJ97797, 1:400), ATR (Abcam, Cambridge, UK, ab178407, 1:100), BRCA1 (St John’s Laboratory, STJ113833, 1:300), RAD51 (St John’s Laboratory, STJ95330, 1:100), and BRCA2 (St John’s Laboratory, STJ91885, 1:100).

Techniques: Comparison, Expressing

Journal: Biomarker Insights

Article Title: mRNA Expression and Methylation of the RAD51 , ATM , ATR , BRCA1 , and BRCA2 Genes in Gastric Adenocarcinoma

doi: 10.1177/11772719231225206

Figure Lengend Snippet: Association between immunohistochemistry and mRNA levels in tumor samples.

Article Snippet: The following antibodies were used: ATM (St John’s Laboratory, London, UK, STJ97797, 1:400), ATR (Abcam, Cambridge, UK, ab178407, 1:100), BRCA1 (St John’s Laboratory, STJ113833, 1:300), RAD51 (St John’s Laboratory, STJ95330, 1:100), and BRCA2 (St John’s Laboratory, STJ91885, 1:100).

Techniques: Immunohistochemistry

Journal: Biomarker Insights

Article Title: mRNA Expression and Methylation of the RAD51 , ATM , ATR , BRCA1 , and BRCA2 Genes in Gastric Adenocarcinoma

doi: 10.1177/11772719231225206

Figure Lengend Snippet: Correlation between RAD51 , ATR , BRCA1 , BRCA2 , and ATM mRNA expression and histological type, histological grade, perineural invasion, and vascular invasion in tumor samples.

Article Snippet: The following antibodies were used: ATM (St John’s Laboratory, London, UK, STJ97797, 1:400), ATR (Abcam, Cambridge, UK, ab178407, 1:100), BRCA1 (St John’s Laboratory, STJ113833, 1:300), RAD51 (St John’s Laboratory, STJ95330, 1:100), and BRCA2 (St John’s Laboratory, STJ91885, 1:100).

Techniques: Expressing

Journal: Biomarker Insights

Article Title: mRNA Expression and Methylation of the RAD51 , ATM , ATR , BRCA1 , and BRCA2 Genes in Gastric Adenocarcinoma

doi: 10.1177/11772719231225206

Figure Lengend Snippet: Correlation between RAD51 , ATR , BRCA1 , BRCA2 , and ATM mRNA expression and depth of invasion (pT), regional lymph node metastasis, distant metastasis, and stage in tumor samples.

Article Snippet: The following antibodies were used: ATM (St John’s Laboratory, London, UK, STJ97797, 1:400), ATR (Abcam, Cambridge, UK, ab178407, 1:100), BRCA1 (St John’s Laboratory, STJ113833, 1:300), RAD51 (St John’s Laboratory, STJ95330, 1:100), and BRCA2 (St John’s Laboratory, STJ91885, 1:100).

Techniques: Expressing

Journal: Biomarker Insights

Article Title: mRNA Expression and Methylation of the RAD51 , ATM , ATR , BRCA1 , and BRCA2 Genes in Gastric Adenocarcinoma

doi: 10.1177/11772719231225206

Figure Lengend Snippet: Comparison between the mRNA levels in the tumors of patients who died and those who did not, considering the following groups: total population, excluding neoadjuvant therapy, with only surgical treatment, and with adjuvant or neoadjuvant treatment.

Article Snippet: The following antibodies were used: ATM (St John’s Laboratory, London, UK, STJ97797, 1:400), ATR (Abcam, Cambridge, UK, ab178407, 1:100), BRCA1 (St John’s Laboratory, STJ113833, 1:300), RAD51 (St John’s Laboratory, STJ95330, 1:100), and BRCA2 (St John’s Laboratory, STJ91885, 1:100).

Techniques: Comparison, Adjuvant

Journal: Biomarker Insights

Article Title: mRNA Expression and Methylation of the RAD51 , ATM , ATR , BRCA1 , and BRCA2 Genes in Gastric Adenocarcinoma

doi: 10.1177/11772719231225206

Figure Lengend Snippet: Kaplan-Meier curves comparing the overall survival of the 24 patients with tumor mRNA expression above the median with that of the 24 below the median, for RAD51 , ATR , BRCA1 , BRCA2 , and ATM .

Article Snippet: The following antibodies were used: ATM (St John’s Laboratory, London, UK, STJ97797, 1:400), ATR (Abcam, Cambridge, UK, ab178407, 1:100), BRCA1 (St John’s Laboratory, STJ113833, 1:300), RAD51 (St John’s Laboratory, STJ95330, 1:100), and BRCA2 (St John’s Laboratory, STJ91885, 1:100).

Techniques: Expressing

Journal: Oncology Letters

Article Title: Expression characteristics of FHIT , p53 , BRCA2 and MLH1 in families with a history of oesophageal cancer in a region with a high incidence of oesophageal cancer

doi: 10.3892/ol.2014.2682

Figure Lengend Snippet: Breast cancer type 2 susceptibility protein (BRCA2) immune response in oesophageal carcinoma tissues. (A) Strong and positive reaction for BRCA2 expression. Immunoreactivity was predominantly located in the cytoplasm and cell membrane; a positive cancer cell is shown by the arrow. (B) Weak and positive reaction for BRCA2 expression. Immunoreactivity was predominantly located in the cytoplasm and membrane; a positive cancer cell is shown by the arrow. (C) Negative expression for BRCA2; a negatively expressed cell is shown by the arrow. Magnification, ×400; haematoxylin and eosin restained.

Article Snippet: Subsequently, 0.5% H 2 O 2 was added to MLH1 at room temperature for 20 min and the resulting mixture was washed three times with PBS for 5 min. BRCA2 rabbit anti-human polyclonal antibody was purchased from Boster Biological Engineering Co., Ltd., (Wuhan, China; dilution 1:100), MLH1 rat anti-horse monoclonal antibody was purchased from BD Pharmingen (San Diego, CA, USA; dilution, 1:50), the FHIT polyclonal rabbit anti-goat antibody was purchased from Beijing Zhongshan Biotechnology Co., Ltd. (Beijing, China) (ZA-0410; 1:100 dilution) and p53 rat anti-horse monoclonal antibody (1:1,000) was purchased from Nuclea Biotechnologies, Inc., (Pittsfield, MA, USA).

Techniques: Expressing, Membrane

Journal: Oncology Letters

Article Title: Expression characteristics of FHIT , p53 , BRCA2 and MLH1 in families with a history of oesophageal cancer in a region with a high incidence of oesophageal cancer

doi: 10.3892/ol.2014.2682

Figure Lengend Snippet: Correlation analysis of BRCA2 , p53 , MLH1 and FHIT expression in the cancer tissues of oesophageal carcinoma patients with or without a family history of oesophageal cancer.

Article Snippet: Subsequently, 0.5% H 2 O 2 was added to MLH1 at room temperature for 20 min and the resulting mixture was washed three times with PBS for 5 min. BRCA2 rabbit anti-human polyclonal antibody was purchased from Boster Biological Engineering Co., Ltd., (Wuhan, China; dilution 1:100), MLH1 rat anti-horse monoclonal antibody was purchased from BD Pharmingen (San Diego, CA, USA; dilution, 1:50), the FHIT polyclonal rabbit anti-goat antibody was purchased from Beijing Zhongshan Biotechnology Co., Ltd. (Beijing, China) (ZA-0410; 1:100 dilution) and p53 rat anti-horse monoclonal antibody (1:1,000) was purchased from Nuclea Biotechnologies, Inc., (Pittsfield, MA, USA).

Techniques: Expressing

Journal: Oncology Letters

Article Title: Expression characteristics of FHIT , p53 , BRCA2 and MLH1 in families with a history of oesophageal cancer in a region with a high incidence of oesophageal cancer

doi: 10.3892/ol.2014.2682

Figure Lengend Snippet: Correlation analysis of BRCA2 , p53 and MLH1 expression in the cancer tissues of oesophageal carcinoma patients with or without a family history of oesophageal cancer.

Article Snippet: Subsequently, 0.5% H 2 O 2 was added to MLH1 at room temperature for 20 min and the resulting mixture was washed three times with PBS for 5 min. BRCA2 rabbit anti-human polyclonal antibody was purchased from Boster Biological Engineering Co., Ltd., (Wuhan, China; dilution 1:100), MLH1 rat anti-horse monoclonal antibody was purchased from BD Pharmingen (San Diego, CA, USA; dilution, 1:50), the FHIT polyclonal rabbit anti-goat antibody was purchased from Beijing Zhongshan Biotechnology Co., Ltd. (Beijing, China) (ZA-0410; 1:100 dilution) and p53 rat anti-horse monoclonal antibody (1:1,000) was purchased from Nuclea Biotechnologies, Inc., (Pittsfield, MA, USA).

Techniques: Expressing

Journal: Oncology Letters

Article Title: Expression characteristics of FHIT , p53 , BRCA2 and MLH1 in families with a history of oesophageal cancer in a region with a high incidence of oesophageal cancer

doi: 10.3892/ol.2014.2682

Figure Lengend Snippet: Association between BRCA2, MLH1 , FHIT and p53 positive expression and clinicopathological characteristics of oesophageal carcinoma.

Article Snippet: Subsequently, 0.5% H 2 O 2 was added to MLH1 at room temperature for 20 min and the resulting mixture was washed three times with PBS for 5 min. BRCA2 rabbit anti-human polyclonal antibody was purchased from Boster Biological Engineering Co., Ltd., (Wuhan, China; dilution 1:100), MLH1 rat anti-horse monoclonal antibody was purchased from BD Pharmingen (San Diego, CA, USA; dilution, 1:50), the FHIT polyclonal rabbit anti-goat antibody was purchased from Beijing Zhongshan Biotechnology Co., Ltd. (Beijing, China) (ZA-0410; 1:100 dilution) and p53 rat anti-horse monoclonal antibody (1:1,000) was purchased from Nuclea Biotechnologies, Inc., (Pittsfield, MA, USA).

Techniques: Expressing

Journal: Oncology Letters

Article Title: Expression characteristics of FHIT , p53 , BRCA2 and MLH1 in families with a history of oesophageal cancer in a region with a high incidence of oesophageal cancer

doi: 10.3892/ol.2014.2682

Figure Lengend Snippet: BRCA2 and MLH1 expression analysis in patients with a positive or negative family history of oesophageal carcinoma.

Article Snippet: Subsequently, 0.5% H 2 O 2 was added to MLH1 at room temperature for 20 min and the resulting mixture was washed three times with PBS for 5 min. BRCA2 rabbit anti-human polyclonal antibody was purchased from Boster Biological Engineering Co., Ltd., (Wuhan, China; dilution 1:100), MLH1 rat anti-horse monoclonal antibody was purchased from BD Pharmingen (San Diego, CA, USA; dilution, 1:50), the FHIT polyclonal rabbit anti-goat antibody was purchased from Beijing Zhongshan Biotechnology Co., Ltd. (Beijing, China) (ZA-0410; 1:100 dilution) and p53 rat anti-horse monoclonal antibody (1:1,000) was purchased from Nuclea Biotechnologies, Inc., (Pittsfield, MA, USA).

Techniques: Expressing

Journal: International Journal of Biological Sciences

Article Title: BRCA1 Antibodies Matter

doi: 10.7150/ijbs.63115

Figure Lengend Snippet: Validation of BRCA1 antibodies in western blot application with human cancer cell lines. (A) BRCA1 Abs test with MDA-MB-468 cells with/without siRNA transfection. (B) BRCA1 Ab test with U2OS cells with/without BRCA1 siRNA transfection. (C) BRCA1 Ab test with Hela inducible BRCA1 KO cell lines with/without IR.

Article Snippet: BRCA1 (Ab5) , Oncogene Science , AP16.

Techniques: Biomarker Discovery, Western Blot, Transfection

Journal: International Journal of Biological Sciences

Article Title: BRCA1 Antibodies Matter

doi: 10.7150/ijbs.63115

Figure Lengend Snippet: Analysis of BRCA1 antibodies by immunoprecipitation in human cell lines. (A) BRCA1 IPs with 293T/3XFlag-hBRCA1 stably transfected cell lysate. (B) BRCA1 IPs with U2OS/3xFLAG-APEX2-fused BRCA1 cell lysate. (C) BRCA1 IPs with MDA-MB-468 cell lysate.

Article Snippet: BRCA1 (Ab5) , Oncogene Science , AP16.

Techniques: Immunoprecipitation, Stable Transfection, Transfection

Journal: International Journal of Biological Sciences

Article Title: BRCA1 Antibodies Matter

doi: 10.7150/ijbs.63115

Figure Lengend Snippet: Analysis of BRCA1 antibodies by immunofluorescence in human U2OS cells. Laser stripes of BRCA1 and γH2AX at 1h time point after laser micro-irradiation in U2OS cells.

Article Snippet: BRCA1 (Ab5) , Oncogene Science , AP16.

Techniques: Immunofluorescence, Irradiation

Journal: International Journal of Biological Sciences

Article Title: BRCA1 Antibodies Matter

doi: 10.7150/ijbs.63115

Figure Lengend Snippet: Analysis of BRCA1 antibodies by ChIP-qPCR in human cancer cells. (A) BRCA1 immunoblots with CTT20 (inducible Cas9 in HeLa), A9.2 and A10.2 (two BRCA1 inducible KO cell lines) cells with/without doxycycline treatment. (B-F) ChIP-qPCR profiles of CTT20 (inducible Cas9 in HeLa), A9.2 and A10.2 cells with/without doxycycline treatment using BRCA1(Ab1) antibody at the BRCA1 (B), ATRIP (C), EXO1 (D), MAD2L1 (E) and PPM1D (F) promoter regions. 6 μg of Ab (if concentration of Ab is provided) or 15 μl of serum (if concentration is not provided) is used. **p < 0.01, *p < 0.05, Student's t-test.

Article Snippet: BRCA1 (Ab5) , Oncogene Science , AP16.

Techniques: ChIP-qPCR, Western Blot, Concentration Assay

Journal: International Journal of Biological Sciences

Article Title: BRCA1 Antibodies Matter

doi: 10.7150/ijbs.63115

Figure Lengend Snippet: Validation of BRCA1 antibodies in Western blot with mouse testis and MEC cells. (A) A representative 8-week-old BRCA1 Δ11/Δ11 mouse on the right was smaller compared with its WT littermate on the left in the p53+/- background. (B) The spleen and testis isolated from BRCA1 Δ11/Δ11 and WT littermate male mice at 2-month old. (C) BRCA1 WB with testis tissue lysate from BRCA1 Δ11/Δ11 and WT littermate male mice at 2-month old. (D) BRCA1 WB with primary mouse mammary epithelial cell lysate from BRCA1 Δ11/Δ11 and WT littermate female mice at 6-week old.

Article Snippet: BRCA1 (Ab5) , Oncogene Science , AP16.

Techniques: Biomarker Discovery, Western Blot, Isolation

Journal: International Journal of Biological Sciences

Article Title: BRCA1 Antibodies Matter

doi: 10.7150/ijbs.63115

Figure Lengend Snippet: Validation of BRCA1 antibodies in MEF cells. (A) MEF cell lysates were prepared and immunoprecipitation was performed using the indicated BRCA1 antibodies pre-coupled to protein A/G beads. (B) Summary of BRCA1 specific peptides detected by MS in IP samples with two anti-BRCA1 antibodies.

Article Snippet: BRCA1 (Ab5) , Oncogene Science , AP16.

Techniques: Biomarker Discovery, Immunoprecipitation

Journal: International Journal of Biological Sciences

Article Title: BRCA1 Antibodies Matter

doi: 10.7150/ijbs.63115

Figure Lengend Snippet: Analysis of BRCA1 antibodies by immunofluorescence in MEF cells. Laser stripes of BRCA1 and γH2AX at 1h time point after laser micro-irradiation in MEF cells.

Article Snippet: BRCA1 (Ab5) , Oncogene Science , AP16.

Techniques: Immunofluorescence, Irradiation

Journal: International Journal of Biological Sciences

Article Title: BRCA1 Antibodies Matter

doi: 10.7150/ijbs.63115

Figure Lengend Snippet: Antibodies used in this study

Article Snippet: BRCA1 (Ab5) , Oncogene Science , AP16.

Techniques:

Journal: Acta Biochimica et Biophysica Sinica

Article Title: CD47 blockade enhances cisplatin sensitivity by inhibiting DNA repair gene expression

doi: 10.3724/abbs.2025147

Figure Lengend Snippet: CD47 regulates the expression of DNA damage repair genes (A) HepG2 cells were treated with cisplatin (10 μM) for 48 h. After treatment, the mRNA levels of indicated DNA repair genes were determined by qRT-PCR. (B) HepG2 cells were treated with cisplatin as described above. The protein levels of FANCA, BRCA2, and ERCC1 were determined. (C) Cells were seeded onto 96-well plates. After 24 h, cisplatin (10 μM) was added and cell incubation continued. Cell viability was detected at indicated time points. (D) HepG2 control and CD47-KD cells expressing Flag-ERCC1 or Flag-FANCA were treated with cisplatin 10 μM for 48 h, followed by western blot analysis. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, CD47-KD/cisplatin vs CD47 -KD/cisplatin/ERCC1/FANCA.

Article Snippet: FANCA and BRCA2 antibodies were from HUABIO (Hangzhou, China).

Techniques: Expressing, Quantitative RT-PCR, Incubation, Control, Western Blot

Journal: Acta Biochimica et Biophysica Sinica

Article Title: CD47 blockade enhances cisplatin sensitivity by inhibiting DNA repair gene expression

doi: 10.3724/abbs.2025147

Figure Lengend Snippet: CD47 blockade enhances inhibitory efficacy of cisplatin on liver tumor growth (A,B) Liver cancer MHCC97 cells were implanted onto nude mice and the mice were treated as described in Methods. Tumors (A) and tumor growth kinetics (B) are shown. (C) Expression of Cleaved Caspase 3 and γH2AX in tumors. (D) Expression of FANCA, BRCA2, and ERCC1 in tumors. **P < 0.01, cisplatin vs anti-CD47/cisplatin.

Article Snippet: FANCA and BRCA2 antibodies were from HUABIO (Hangzhou, China).

Techniques: Expressing

Journal: Food Science & Nutrition

Article Title: Exploring the Mechanisms of Total Saponins of Black Ginseng and Ginsenoside Rg3 Against Doxorubicin‐Induced Cardiotoxicity

doi: 10.1002/fsn3.71968

Figure Lengend Snippet: The potential function and pathways of TSF on DOX‐induced cardiotoxicity: (a) PPI network of 103 overlapping targets constructed based on the STRING database; (b) the PPI network map; (c) the molecular docking of HDAC8 with BRCA2.

Article Snippet: Antibodies for BRCA2, DRP1 and Bcl‐2 were sourced from Wanlei Biotechnology Co. Ltd. (Lot: T11052438, T09113028, T11201556), while β ‐actin and HRP‐labeled secondary antibodies were procured from Affinity Biosciences (Lot: T0022, S0001).

Techniques: Construct

Journal: Food Science & Nutrition

Article Title: Exploring the Mechanisms of Total Saponins of Black Ginseng and Ginsenoside Rg3 Against Doxorubicin‐Induced Cardiotoxicity

doi: 10.1002/fsn3.71968

Figure Lengend Snippet: TSF and Rg3 regulate HDAC8/BRCA2/DRP1 pathway inhibits DOX‐induced cardiomyocyte apoptosis. (a) Apoptosis was assessed using TUNEL staining (Scale bar = 50 μm). (b) The results of western blotting for each group. (c) The expression of Bcl‐2 for each group. (d) The expression of DRP1 for each group. (e) The expression of HDAC8 for each group. (f) The expression of BRCA2 for each group. (g) Immunofluorescence staining for observing the colocalization of HDAC8, BRCA2, and DRP1 (Scale bar = 1 mm). Data are expressed as mean ± standard from three individual experiments. Data analysis among groups was performed using ANOVA, with LSD for equal variances and Dunnett’s‐T3 for unequal variances, with significance levels of * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Antibodies for BRCA2, DRP1 and Bcl‐2 were sourced from Wanlei Biotechnology Co. Ltd. (Lot: T11052438, T09113028, T11201556), while β ‐actin and HRP‐labeled secondary antibodies were procured from Affinity Biosciences (Lot: T0022, S0001).

Techniques: TUNEL Assay, Staining, Western Blot, Expressing, Immunofluorescence

Journal: Food Science & Nutrition

Article Title: Exploring the Mechanisms of Total Saponins of Black Ginseng and Ginsenoside Rg3 Against Doxorubicin‐Induced Cardiotoxicity

doi: 10.1002/fsn3.71968

Figure Lengend Snippet: The mechanism diagram of TSF and Rg3 alleviating DOX‐induced myocardial injury by regulating the HDAC8/BRCA2/DRP1 pathway.

Article Snippet: Antibodies for BRCA2, DRP1 and Bcl‐2 were sourced from Wanlei Biotechnology Co. Ltd. (Lot: T11052438, T09113028, T11201556), while β ‐actin and HRP‐labeled secondary antibodies were procured from Affinity Biosciences (Lot: T0022, S0001).

Techniques: