Journal: Protein science : a publication of the Protein Society

Article Title: Effects of mutation at the D-JH junction on affinity, specificity, and idiotypy of anti-progesterone antibody DB3.

doi: 10.1110/ps.062236806

Figure Lengend Snippet: Figure 2. Structure of the DB3 combining site. (A) Crystal structure of the complex between DB3 and progesterone (Arevalo et al. 1993a) showing some of the contact residues. Light and the heavy chains are in blue and purple, respectively; steroid oxygen atoms in red; steroid A-ring asterisked. (B) Model of site with TrpH100 replaced by arginine. (C) Superposition of A and B.

Article Snippet: Antibody DB3 engineering JOBNAME: PROSCI 15#9 2006 PAGE: 3 OUTPUT: Saturday August 5 02:47:19 2006 csh/PROSCI/118160/ps0622368 1469896x, 2006, 9, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1110/ps.062236806 by U niversity O f T he Philipines D ilim an M ain L ibrary, W iley O nline L ibrary on [05/04/2024].

Techniques:

Journal: Protein science : a publication of the Protein Society

Article Title: Effects of mutation at the D-JH junction on affinity, specificity, and idiotypy of anti-progesterone antibody DB3.

doi: 10.1110/ps.062236806

Figure Lengend Snippet: Figure 3. Western blot of wild type and mutants of DB3 VH/K. Samples were run on 10% SDS-PAGE and electrotransferred to an Immobilon-P membrane (PVDF); the blot was probed with HRP-coupled sheep anti- mouse k.

Article Snippet: Antibody DB3 engineering JOBNAME: PROSCI 15#9 2006 PAGE: 3 OUTPUT: Saturday August 5 02:47:19 2006 csh/PROSCI/118160/ps0622368 1469896x, 2006, 9, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1110/ps.062236806 by U niversity O f T he Philipines D ilim an M ain L ibrary, W iley O nline L ibrary on [05/04/2024].

Techniques: Western Blot, SDS Page, Membrane

Journal: Protein science : a publication of the Protein Society

Article Title: Effects of mutation at the D-JH junction on affinity, specificity, and idiotypy of anti-progesterone antibody DB3.

doi: 10.1110/ps.062236806

Figure Lengend Snippet: Figure 4. Antigen-binding and idiotypic properties of periplasmic extracts of E. coli expressing VH/K mutants. (A) Binding activity by ELISA on progesterone-BSA coated wells; (B) Competitive inhibition of binding by free progesterone (100 ng/mL) in ELISA; (C) Competitive inhibition by rabbit polyclonal anti-DB3-idiotype (10 mg/mL). Results are means of duplicates. (d) Deletion; (Co.) negative control.

Article Snippet: Antibody DB3 engineering JOBNAME: PROSCI 15#9 2006 PAGE: 3 OUTPUT: Saturday August 5 02:47:19 2006 csh/PROSCI/118160/ps0622368 1469896x, 2006, 9, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1110/ps.062236806 by U niversity O f T he Philipines D ilim an M ain L ibrary, W iley O nline L ibrary on [05/04/2024].

Techniques: Binding Assay, Expressing, Activity Assay, Enzyme-linked Immunosorbent Assay, Inhibition, Negative Control

Journal: Protein science : a publication of the Protein Society

Article Title: Effects of mutation at the D-JH junction on affinity, specificity, and idiotypy of anti-progesterone antibody DB3.

doi: 10.1110/ps.062236806

Figure Lengend Snippet: Figure 5. Steroid specificity of DB3 wild-type VH/K and TrpH100Arg mutant. Binding to progesterone-BSA of fixed amounts of wild-type DB3 VH/K (A) and the TrpH100Arg mutant (B) was carried out in the presence of free progesterone, aetiocholanolone, 5a-dihydroprogesterone, 5b-dihydroprogesterone, or testosterone as inhibitors. Results are means of duplicates.

Article Snippet: Antibody DB3 engineering JOBNAME: PROSCI 15#9 2006 PAGE: 3 OUTPUT: Saturday August 5 02:47:19 2006 csh/PROSCI/118160/ps0622368 1469896x, 2006, 9, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1110/ps.062236806 by U niversity O f T he Philipines D ilim an M ain L ibrary, W iley O nline L ibrary on [05/04/2024].

Techniques: Mutagenesis, Binding Assay

Journal: Protein science : a publication of the Protein Society

Article Title: Effects of mutation at the D-JH junction on affinity, specificity, and idiotypy of anti-progesterone antibody DB3.

doi: 10.1110/ps.062236806

Figure Lengend Snippet: Figure 6. Binding specificity of wild-type DB3 VH/K and the TrpH100Arg mutant for progesterone derivatives. Binding to progester- one-BSA of wild-type DB3 VH/K (A) and the TrpH100Arg mutant (B) was carried out in the presence of free progesterone-3-CMO (C3-CMO), progesterone-6-HMS (C6-HMS), progesterone-11a-HMS (C11a-HMS), or progesterone-21-HMS (C21-HMS). Results are means of duplicates.

Article Snippet: Antibody DB3 engineering JOBNAME: PROSCI 15#9 2006 PAGE: 3 OUTPUT: Saturday August 5 02:47:19 2006 csh/PROSCI/118160/ps0622368 1469896x, 2006, 9, D ow nloaded from https://onlinelibrary.w iley.com /doi/10.1110/ps.062236806 by U niversity O f T he Philipines D ilim an M ain L ibrary, W iley O nline L ibrary on [05/04/2024].

Techniques: Binding Assay, Mutagenesis

Journal: Frontiers in pharmacology

Article Title: Fruits of Hippophaë rhamnoides in human leukocytes and Caco-2 cell monolayer models-A question about their preventive role in lipopolysaccharide leakage and cytokine secretion in endotoxemia.

doi: 10.3389/fphar.2022.981874

Figure Lengend Snippet: FIGURE 5 Detection of GLUT2 translocation in Caco-2 cells in a fluorescence microscope. HR—aqueous extract from the fruit of Hipopphaë rhamnoides.

Article Snippet: Glut2 polyclonal antibody (600-401-GN3, Rockland, PA, United States), FITCconjugated goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody (F-2765, Invitrogen, United Kingdom), and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; D1306) were purchased from Thermo Fisher Scientific (Rockford, IL, United States).

Techniques: Translocation Assay, Microscopy