Journal: BioMed Research International

Article Title: Isolation, Characterization, and Effect on Biofilm Formation of Bacteriocin Produced by Lactococcus lactis F01 Isolated from Cyprinus carpio and Application for Biopreservation of Fish Sausage

doi: 10.1155/2022/8437926

Figure Lengend Snippet: Antimicrobial spectrum of C4B.

Article Snippet: According to these results, C4B has a bactericidal effect against Salmonella Typhimurium ATCC 14028 and Staphylococcus aureus .

Techniques: Activity Assay

Journal: BioMed Research International

Article Title: Isolation, Characterization, and Effect on Biofilm Formation of Bacteriocin Produced by Lactococcus lactis F01 Isolated from Cyprinus carpio and Application for Biopreservation of Fish Sausage

doi: 10.1155/2022/8437926

Figure Lengend Snippet: Effect of C4B on the growth of Salmonella Typhimurium ATCC 14028. Bact: bacteriocin.

Article Snippet: According to these results, C4B has a bactericidal effect against Salmonella Typhimurium ATCC 14028 and Staphylococcus aureus .

Techniques:

Journal: Foods

Article Title: Edible Films Made of Dried Olive Leaf Extract and Chitosan: Characterization and Applications

doi: 10.3390/foods11142078

Figure Lengend Snippet: Antimicrobial activity of DOLE-based films. Antimicrobial effects were evaluated by analyzing the growth of the bacterial cells held in direct contact with the films ( a ). The diameter of the inhibition circle was determined for each bacterial strain in contact with the different films and shown in the graph ( b ). The experiments were carried out in triplicate. Statistical analyses were performed by using Student’s t -test. Significant differences were indicated as * p < 0.05 or ** p < 0.01.

Article Snippet: Thus, the antimicrobial effect of DOLE-containing films against bacterial strains ( Salmonella enterica subsp. enterica serovar Typhimurium ATCC ® 14028, Salmonella enteritidis RIVM 706, and Enterococcus faecalis ATCC ® 29212) was tested in vitro.

Techniques: Activity Assay, Inhibition

Journal: Frontiers in Microbiology

Article Title: Relevant Genes Linked to Virulence Are Required for Salmonella Typhimurium to Survive Intracellularly in the Social Amoeba Dictyostelium discoideum

doi: 10.3389/fmicb.2016.01305

Figure Lengend Snippet: Bacterial and Dictyostelium strains used in this study.

Article Snippet: S. Typhimurium 14028s and E. coli B/r were transformed by electroporation with plasmid pFCcGi (Addgene plasmid number 59324), encoding the red fluorescent protein mCherry expressed constitutively ( ).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Relevant Genes Linked to Virulence Are Required for Salmonella Typhimurium to Survive Intracellularly in the Social Amoeba Dictyostelium discoideum

doi: 10.3389/fmicb.2016.01305

Figure Lengend Snippet: Intracellular survival of S. Typhimurium 14028s and E. coli B/r in D. discoideum. Individual infection assays were used to determine the intracellular survival of S. Typhimurium 14028s or E. coli B/r in D. discoideum AX4. (A) Intracellular survival calculated at each time point as CFU t=x /CFU t=0 . (B) Population of viable amoebae at each time point expressed as cells/mL. Graphics shows the mean of at least three independent assays ± SEM. Statistical significance was determined using a two-way ANOVA with Fisher’s LSD post-test ( ∗∗ P < 0.005, ∗∗∗ P < 0.001).

Article Snippet: S. Typhimurium 14028s and E. coli B/r were transformed by electroporation with plasmid pFCcGi (Addgene plasmid number 59324), encoding the red fluorescent protein mCherry expressed constitutively ( ).

Techniques: Infection

Journal: Frontiers in Microbiology

Article Title: Relevant Genes Linked to Virulence Are Required for Salmonella Typhimurium to Survive Intracellularly in the Social Amoeba Dictyostelium discoideum

doi: 10.3389/fmicb.2016.01305

Figure Lengend Snippet: Analysis of D. discoideum infected with S. Typhimurium 14028s and E. coli B/r by confocal microscopy. Individual infection assays were used to determine the intracellular fate of S. Typhimurium 14028s pFCcGi or E. coli B/r pFCcGi in D. discoideum AX2 cnxA-GFP. Images of infected cells were acquired at different time points (1, 5, 15, and 23 h post infection) using a Zeiss LSM 710 laser scanning confocal microscope. The complete set of images captured during the experiment is shown in Supplementary Figure .

Article Snippet: S. Typhimurium 14028s and E. coli B/r were transformed by electroporation with plasmid pFCcGi (Addgene plasmid number 59324), encoding the red fluorescent protein mCherry expressed constitutively ( ).

Techniques: Infection, Confocal Microscopy, Microscopy

Journal: Frontiers in Microbiology

Article Title: Relevant Genes Linked to Virulence Are Required for Salmonella Typhimurium to Survive Intracellularly in the Social Amoeba Dictyostelium discoideum

doi: 10.3389/fmicb.2016.01305

Figure Lengend Snippet: Internalization and intracellular survival of S. Typhimurium 14028s and defined mutants in D. discoideum. Competition assays were used to determine the intracellular survival of S. Typhimurium mutants Δ aroA (purple), Δ invA (green), Δ ssaD (red) Δ clpV (blue), Δ phoPQ (orange), Δ phoN (light blue), and Δ waaL (yellow). (A) Internalization relative to the wild-type strain calculated as (CFU mutant /CFU WT ) t=0 /(CFU mutant /CFU WT ) inoculum and expressed as log 10 . (B) Competitive index (CI) between a defined mutant and the wild-type strain calculated at each time point as (CFU mutant /CFU WT ) t = x /(CFU mutant /CFU WT ) t=0 and expressed as log 10 . (C) Population of viable amoebae at each time point expressed as cells/mL. Graphics shows the mean of at least three independent assays ± SEM. Statistical significance was determined using a two-tailed Student’s t -test ( ∗ P < 0.05, ∗∗ P < 0.005, ∗∗∗ P < 0.001).

Article Snippet: S. Typhimurium 14028s and E. coli B/r were transformed by electroporation with plasmid pFCcGi (Addgene plasmid number 59324), encoding the red fluorescent protein mCherry expressed constitutively ( ).

Techniques: Mutagenesis, Two Tailed Test