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  • 98
    Vector Laboratories anti rat secondary ab
    Anti Rat Secondary Ab, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher anti rat foxp3 abs
    hIL-17 treatment results in enhanced regulatory T cell activity. A , Rats treated with IL-17 express increased numbers of <t>Foxp3</t> + T cells in the periphery and in the inflamed eye. Lewis rats were immunized with R16 with or without IL-17 treatment (two i.p. injections of 3 µg/injection on days 4 and 7), then splenic T cells and eye-infiltrating cells were prepared on day 10 p.i. and the number of CD4 + Foxp3 + T cells were assessed by dual staining with anti-CD4 and anti-Foxp3 Abs and FACS analysis. B , Splenic T cells from hIL-17-treated rats have increased suppressor activity. Two groups ( n = 4) of rats were immunized with a pathogenic dose of R16 (50 µg/rat) and one group was also injected i.p. with 50 × 10 6 splenic T cells isolated on day 15 from R16-immunized rats treated with IL-17 on days 4 and 7 or from R16-immunized rats without IL-17 treatment (control). Disease was monitored and scored. The results shown are the mean ± SD from one representative experiment of two.
    Anti Rat Foxp3 Abs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    SouthernBiotech anti rat abs
    hIL-17 treatment results in enhanced regulatory T cell activity. A , Rats treated with IL-17 express increased numbers of <t>Foxp3</t> + T cells in the periphery and in the inflamed eye. Lewis rats were immunized with R16 with or without IL-17 treatment (two i.p. injections of 3 µg/injection on days 4 and 7), then splenic T cells and eye-infiltrating cells were prepared on day 10 p.i. and the number of CD4 + Foxp3 + T cells were assessed by dual staining with anti-CD4 and anti-Foxp3 Abs and FACS analysis. B , Splenic T cells from hIL-17-treated rats have increased suppressor activity. Two groups ( n = 4) of rats were immunized with a pathogenic dose of R16 (50 µg/rat) and one group was also injected i.p. with 50 × 10 6 splenic T cells isolated on day 15 from R16-immunized rats treated with IL-17 on days 4 and 7 or from R16-immunized rats without IL-17 treatment (control). Disease was monitored and scored. The results shown are the mean ± SD from one representative experiment of two.
    Anti Rat Abs, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher anti rat a546 abs
    hIL-17 treatment results in enhanced regulatory T cell activity. A , Rats treated with IL-17 express increased numbers of <t>Foxp3</t> + T cells in the periphery and in the inflamed eye. Lewis rats were immunized with R16 with or without IL-17 treatment (two i.p. injections of 3 µg/injection on days 4 and 7), then splenic T cells and eye-infiltrating cells were prepared on day 10 p.i. and the number of CD4 + Foxp3 + T cells were assessed by dual staining with anti-CD4 and anti-Foxp3 Abs and FACS analysis. B , Splenic T cells from hIL-17-treated rats have increased suppressor activity. Two groups ( n = 4) of rats were immunized with a pathogenic dose of R16 (50 µg/rat) and one group was also injected i.p. with 50 × 10 6 splenic T cells isolated on day 15 from R16-immunized rats treated with IL-17 on days 4 and 7 or from R16-immunized rats without IL-17 treatment (control). Disease was monitored and scored. The results shown are the mean ± SD from one representative experiment of two.
    Anti Rat A546 Abs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher anti rat antibodies
    hIL-17 treatment results in enhanced regulatory T cell activity. A , Rats treated with IL-17 express increased numbers of <t>Foxp3</t> + T cells in the periphery and in the inflamed eye. Lewis rats were immunized with R16 with or without IL-17 treatment (two i.p. injections of 3 µg/injection on days 4 and 7), then splenic T cells and eye-infiltrating cells were prepared on day 10 p.i. and the number of CD4 + Foxp3 + T cells were assessed by dual staining with anti-CD4 and anti-Foxp3 Abs and FACS analysis. B , Splenic T cells from hIL-17-treated rats have increased suppressor activity. Two groups ( n = 4) of rats were immunized with a pathogenic dose of R16 (50 µg/rat) and one group was also injected i.p. with 50 × 10 6 splenic T cells isolated on day 15 from R16-immunized rats treated with IL-17 on days 4 and 7 or from R16-immunized rats without IL-17 treatment (control). Disease was monitored and scored. The results shown are the mean ± SD from one representative experiment of two.
    Anti Rat Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 361 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Santa Cruz Biotechnology anti rat bd3 abs
    hIL-17 treatment results in enhanced regulatory T cell activity. A , Rats treated with IL-17 express increased numbers of <t>Foxp3</t> + T cells in the periphery and in the inflamed eye. Lewis rats were immunized with R16 with or without IL-17 treatment (two i.p. injections of 3 µg/injection on days 4 and 7), then splenic T cells and eye-infiltrating cells were prepared on day 10 p.i. and the number of CD4 + Foxp3 + T cells were assessed by dual staining with anti-CD4 and anti-Foxp3 Abs and FACS analysis. B , Splenic T cells from hIL-17-treated rats have increased suppressor activity. Two groups ( n = 4) of rats were immunized with a pathogenic dose of R16 (50 µg/rat) and one group was also injected i.p. with 50 × 10 6 splenic T cells isolated on day 15 from R16-immunized rats treated with IL-17 on days 4 and 7 or from R16-immunized rats without IL-17 treatment (control). Disease was monitored and scored. The results shown are the mean ± SD from one representative experiment of two.
    Anti Rat Bd3 Abs, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Becton Dickinson biotinylated anti rat abs
    hIL-17 treatment results in enhanced regulatory T cell activity. A , Rats treated with IL-17 express increased numbers of <t>Foxp3</t> + T cells in the periphery and in the inflamed eye. Lewis rats were immunized with R16 with or without IL-17 treatment (two i.p. injections of 3 µg/injection on days 4 and 7), then splenic T cells and eye-infiltrating cells were prepared on day 10 p.i. and the number of CD4 + Foxp3 + T cells were assessed by dual staining with anti-CD4 and anti-Foxp3 Abs and FACS analysis. B , Splenic T cells from hIL-17-treated rats have increased suppressor activity. Two groups ( n = 4) of rats were immunized with a pathogenic dose of R16 (50 µg/rat) and one group was also injected i.p. with 50 × 10 6 splenic T cells isolated on day 15 from R16-immunized rats treated with IL-17 on days 4 and 7 or from R16-immunized rats without IL-17 treatment (control). Disease was monitored and scored. The results shown are the mean ± SD from one representative experiment of two.
    Biotinylated Anti Rat Abs, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher goat anti rat antibody
    hIL-17 treatment results in enhanced regulatory T cell activity. A , Rats treated with IL-17 express increased numbers of <t>Foxp3</t> + T cells in the periphery and in the inflamed eye. Lewis rats were immunized with R16 with or without IL-17 treatment (two i.p. injections of 3 µg/injection on days 4 and 7), then splenic T cells and eye-infiltrating cells were prepared on day 10 p.i. and the number of CD4 + Foxp3 + T cells were assessed by dual staining with anti-CD4 and anti-Foxp3 Abs and FACS analysis. B , Splenic T cells from hIL-17-treated rats have increased suppressor activity. Two groups ( n = 4) of rats were immunized with a pathogenic dose of R16 (50 µg/rat) and one group was also injected i.p. with 50 × 10 6 splenic T cells isolated on day 15 from R16-immunized rats treated with IL-17 on days 4 and 7 or from R16-immunized rats without IL-17 treatment (control). Disease was monitored and scored. The results shown are the mean ± SD from one representative experiment of two.
    Goat Anti Rat Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Nanoprobes anti rat igg abs
    Identification of the <t>G213-reactive</t> protein as S100A9 . (A) Tissue lysates derived from the lungs of untreated (left lane) and BCG-challenged (right lane) guinea pigs were resolved on SDS-PAGE, and western blotting was conducted with G213 (upper panel) and Ab to β-actin (lower panel). The 15-kDa species specifically recognized by G213 is indicated with an asterisk. The 30-kDa bands observed on both lanes may represent nonspecific signals. (B) Tissue lysates derived from the bone marrow, spleen, lymph nodes, and thymus of an untreated guinea pig were prepared, and western blotting was performed as in panel A. The 15-kDa species specifically recognized by G213 is indicated with an asterisk. (C) Bone marrow cells obtained from an untreated guinea pig were lysed, and immunoprecipitation (IP) was performed with either control <t>IgG</t> (left lane) or G213 (right lane). Samples were resolved on an SDS-PAGE gel, followed by immunoblotting with G213. The 15-kDa species specifically recognized by G213 is indicated with an asterisk. (D) Cell lysates derived from HEK293T cell transfectants expressing either FLAG-tagged S100A9 or HA-tagged S100A8 as well as mock-treated cells were resolved on SDS-PAGE gels, and immunoblotting with Abs to FLAG, HA, and β-actin as well as G213 mAb was conducted.
    Anti Rat Igg Abs, supplied by Nanoprobes, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Becton Dickinson anti rat cd31 ab
    Effect of vildagliptin on capillary vessels in isoproterenol-treated rat myocardium. <t>CD31</t> staining of hearts in the vehicle, ISO, and ISO-VL groups. Scale bar = 100 μm. Bar graph shows the number of vessels per cardiomyocyte. Values represent the mean ± SE. n = 6 each. NS, not significant.
    Anti Rat Cd31 Ab, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Jackson Immuno anti rat cy3 ab
    Prdm6 affects angiogenic patterning. ( A ) Unusual clusters of densely growing vessel structures on yolk sacs of Prdm6 -deficient embryos, as observed under a stereomicroscope. Scale bars correspond to 1 mm (left panel) or 100 µm (right panel). ( B ) Large vessels in the yolk sacs of E12.5 control and Prdm6 -deficient (del/del) embryos under a stereomicroscope. Scale bars correspond to 500 µm. ( C ) Visualization of E10.5 (left panels) and E11.5 (right panels) yolk sac microvascular systems via immunofluorescent staining with an anti-CD31 primary antibody and a <t>Cy3-conjugated</t> secondary antibody. Scale bars correspond to 200 µm. ( D ) Quantitative morphometric analysis of the yolk sac vasculature as shown in (C). Avascular space and mean vessel diameters of yolk sacs at E10.5 – E 11.5 are shown as mean ± SEM, n=6. More details about this analysis are given in Figure S2 .
    Anti Rat Cy3 Ab, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 85/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    R&D Systems anti rat tlr 4 abs
    Immunohistochemical detection of <t>TLR-4</t> expression in lung tissue, magnification ×400. ( A ) 12W CG group, ( B ) 16W CG group, ( C ) 12W IH group, ( D ) 16W IH group, ( E ) 12W HI group, and ( F ) 16W HI group. * Compared with 12W CG, P
    Anti Rat Tlr 4 Abs, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Santa Cruz Biotechnology anti rat cd68 ab
    Immunohistochemical staining for <t>CD68.</t> CD68 + macrophages are located in the renal interstitium indicated by white arrow, blood vessels indicated by black arrow or the lumen of the renal tubules indicated by red arrow (×400).
    Anti Rat Cd68 Ab, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam polyclonal anti rat c1q ab
    Immunohistochemical staining for <t>CD68.</t> CD68 + macrophages are located in the renal interstitium indicated by white arrow, blood vessels indicated by black arrow or the lumen of the renal tubules indicated by red arrow (×400).
    Polyclonal Anti Rat C1q Ab, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Bio-Rad igg2c abs
    Immunohistochemical staining for <t>CD68.</t> CD68 + macrophages are located in the renal interstitium indicated by white arrow, blood vessels indicated by black arrow or the lumen of the renal tubules indicated by red arrow (×400).
    Igg2c Abs, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    BioLegend apc anti rats cd4
    Immunohistochemical staining for <t>CD68.</t> CD68 + macrophages are located in the renal interstitium indicated by white arrow, blood vessels indicated by black arrow or the lumen of the renal tubules indicated by red arrow (×400).
    Apc Anti Rats Cd4, supplied by BioLegend, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    BioLegend antirat cd45 pe
    Immunohistochemical staining for <t>CD68.</t> CD68 + macrophages are located in the renal interstitium indicated by white arrow, blood vessels indicated by black arrow or the lumen of the renal tubules indicated by red arrow (×400).
    Antirat Cd45 Pe, supplied by BioLegend, used in various techniques. Bioz Stars score: 97/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Accurate Chemical & Scientific Corporation fitc conjugated anti rat abs
    Dam1p is at the kinetochore. ( A ) IF of chromosome spreads from cells containing Ndc10pHA and Dam1p-myc. Blue shows DAPI (DNA), green shows Ndc10pHA detected with anti-HA <t>mAb</t> plus <t>FITC</t> anti-mouse secondary Ab, and red shows Dam1pMyc detected with polyclonal anti-Myc Ab plus Cy3 anti-rabbit secondary Ab. (Bar = 1.5 μm.) ( B ) Dam1-GFP is associated with centromeric DNA in vivo , in an NDC10 -dependent manner. CEN4 association was tested in wild-type or ndc10 - 1 cells at 37°C. Background signal was detected by using URA3 -specific oligonucleotides. Total input, DNA extract of total lysate before immunoprecipitation; IP, DNA extract after immunoprecipitation.
    Fitc Conjugated Anti Rat Abs, supplied by Accurate Chemical & Scientific Corporation, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher anti rat alexa 568 ab
    Dam1p is at the kinetochore. ( A ) IF of chromosome spreads from cells containing Ndc10pHA and Dam1p-myc. Blue shows DAPI (DNA), green shows Ndc10pHA detected with anti-HA <t>mAb</t> plus <t>FITC</t> anti-mouse secondary Ab, and red shows Dam1pMyc detected with polyclonal anti-Myc Ab plus Cy3 anti-rabbit secondary Ab. (Bar = 1.5 μm.) ( B ) Dam1-GFP is associated with centromeric DNA in vivo , in an NDC10 -dependent manner. CEN4 association was tested in wild-type or ndc10 - 1 cells at 37°C. Background signal was detected by using URA3 -specific oligonucleotides. Total input, DNA extract of total lysate before immunoprecipitation; IP, DNA extract after immunoprecipitation.
    Anti Rat Alexa 568 Ab, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Agilent technologies anti rat
    Dam1p is at the kinetochore. ( A ) IF of chromosome spreads from cells containing Ndc10pHA and Dam1p-myc. Blue shows DAPI (DNA), green shows Ndc10pHA detected with anti-HA <t>mAb</t> plus <t>FITC</t> anti-mouse secondary Ab, and red shows Dam1pMyc detected with polyclonal anti-Myc Ab plus Cy3 anti-rabbit secondary Ab. (Bar = 1.5 μm.) ( B ) Dam1-GFP is associated with centromeric DNA in vivo , in an NDC10 -dependent manner. CEN4 association was tested in wild-type or ndc10 - 1 cells at 37°C. Background signal was detected by using URA3 -specific oligonucleotides. Total input, DNA extract of total lysate before immunoprecipitation; IP, DNA extract after immunoprecipitation.
    Anti Rat, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 262 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Cell Signaling Technology Inc anti rat
    Dam1p is at the kinetochore. ( A ) IF of chromosome spreads from cells containing Ndc10pHA and Dam1p-myc. Blue shows DAPI (DNA), green shows Ndc10pHA detected with anti-HA <t>mAb</t> plus <t>FITC</t> anti-mouse secondary Ab, and red shows Dam1pMyc detected with polyclonal anti-Myc Ab plus Cy3 anti-rabbit secondary Ab. (Bar = 1.5 μm.) ( B ) Dam1-GFP is associated with centromeric DNA in vivo , in an NDC10 -dependent manner. CEN4 association was tested in wild-type or ndc10 - 1 cells at 37°C. Background signal was detected by using URA3 -specific oligonucleotides. Total input, DNA extract of total lysate before immunoprecipitation; IP, DNA extract after immunoprecipitation.
    Anti Rat, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    GE Healthcare anti rat
    Dam1p is at the kinetochore. ( A ) IF of chromosome spreads from cells containing Ndc10pHA and Dam1p-myc. Blue shows DAPI (DNA), green shows Ndc10pHA detected with anti-HA <t>mAb</t> plus <t>FITC</t> anti-mouse secondary Ab, and red shows Dam1pMyc detected with polyclonal anti-Myc Ab plus Cy3 anti-rabbit secondary Ab. (Bar = 1.5 μm.) ( B ) Dam1-GFP is associated with centromeric DNA in vivo , in an NDC10 -dependent manner. CEN4 association was tested in wild-type or ndc10 - 1 cells at 37°C. Background signal was detected by using URA3 -specific oligonucleotides. Total input, DNA extract of total lysate before immunoprecipitation; IP, DNA extract after immunoprecipitation.
    Anti Rat, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Jackson Immuno anti rat
    Dam1p is at the kinetochore. ( A ) IF of chromosome spreads from cells containing Ndc10pHA and Dam1p-myc. Blue shows DAPI (DNA), green shows Ndc10pHA detected with anti-HA <t>mAb</t> plus <t>FITC</t> anti-mouse secondary Ab, and red shows Dam1pMyc detected with polyclonal anti-Myc Ab plus Cy3 anti-rabbit secondary Ab. (Bar = 1.5 μm.) ( B ) Dam1-GFP is associated with centromeric DNA in vivo , in an NDC10 -dependent manner. CEN4 association was tested in wild-type or ndc10 - 1 cells at 37°C. Background signal was detected by using URA3 -specific oligonucleotides. Total input, DNA extract of total lysate before immunoprecipitation; IP, DNA extract after immunoprecipitation.
    Anti Rat, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 812 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Santa Cruz Biotechnology anti rat
    Dam1p is at the kinetochore. ( A ) IF of chromosome spreads from cells containing Ndc10pHA and Dam1p-myc. Blue shows DAPI (DNA), green shows Ndc10pHA detected with anti-HA <t>mAb</t> plus <t>FITC</t> anti-mouse secondary Ab, and red shows Dam1pMyc detected with polyclonal anti-Myc Ab plus Cy3 anti-rabbit secondary Ab. (Bar = 1.5 μm.) ( B ) Dam1-GFP is associated with centromeric DNA in vivo , in an NDC10 -dependent manner. CEN4 association was tested in wild-type or ndc10 - 1 cells at 37°C. Background signal was detected by using URA3 -specific oligonucleotides. Total input, DNA extract of total lysate before immunoprecipitation; IP, DNA extract after immunoprecipitation.
    Anti Rat, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 441 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    hIL-17 treatment results in enhanced regulatory T cell activity. A , Rats treated with IL-17 express increased numbers of Foxp3 + T cells in the periphery and in the inflamed eye. Lewis rats were immunized with R16 with or without IL-17 treatment (two i.p. injections of 3 µg/injection on days 4 and 7), then splenic T cells and eye-infiltrating cells were prepared on day 10 p.i. and the number of CD4 + Foxp3 + T cells were assessed by dual staining with anti-CD4 and anti-Foxp3 Abs and FACS analysis. B , Splenic T cells from hIL-17-treated rats have increased suppressor activity. Two groups ( n = 4) of rats were immunized with a pathogenic dose of R16 (50 µg/rat) and one group was also injected i.p. with 50 × 10 6 splenic T cells isolated on day 15 from R16-immunized rats treated with IL-17 on days 4 and 7 or from R16-immunized rats without IL-17 treatment (control). Disease was monitored and scored. The results shown are the mean ± SD from one representative experiment of two.

    Journal: Journal of Immunology (Baltimore, Md. : 1950)

    Article Title: Anti-Inflammatory Role of IL-17 in Experimental Autoimmune Uveitis 1

    doi: 10.4049/jimmunol.0802487

    Figure Lengend Snippet: hIL-17 treatment results in enhanced regulatory T cell activity. A , Rats treated with IL-17 express increased numbers of Foxp3 + T cells in the periphery and in the inflamed eye. Lewis rats were immunized with R16 with or without IL-17 treatment (two i.p. injections of 3 µg/injection on days 4 and 7), then splenic T cells and eye-infiltrating cells were prepared on day 10 p.i. and the number of CD4 + Foxp3 + T cells were assessed by dual staining with anti-CD4 and anti-Foxp3 Abs and FACS analysis. B , Splenic T cells from hIL-17-treated rats have increased suppressor activity. Two groups ( n = 4) of rats were immunized with a pathogenic dose of R16 (50 µg/rat) and one group was also injected i.p. with 50 × 10 6 splenic T cells isolated on day 15 from R16-immunized rats treated with IL-17 on days 4 and 7 or from R16-immunized rats without IL-17 treatment (control). Disease was monitored and scored. The results shown are the mean ± SD from one representative experiment of two.

    Article Snippet: FITC-conjugated anti-mouse IL-17 and PE-conjugated anti-rat IFN-γ, anti-rat Foxp3 Abs were obtained from eBioscience.

    Techniques: Activity Assay, Injection, Staining, FACS, Isolation

    Identification of the G213-reactive protein as S100A9 . (A) Tissue lysates derived from the lungs of untreated (left lane) and BCG-challenged (right lane) guinea pigs were resolved on SDS-PAGE, and western blotting was conducted with G213 (upper panel) and Ab to β-actin (lower panel). The 15-kDa species specifically recognized by G213 is indicated with an asterisk. The 30-kDa bands observed on both lanes may represent nonspecific signals. (B) Tissue lysates derived from the bone marrow, spleen, lymph nodes, and thymus of an untreated guinea pig were prepared, and western blotting was performed as in panel A. The 15-kDa species specifically recognized by G213 is indicated with an asterisk. (C) Bone marrow cells obtained from an untreated guinea pig were lysed, and immunoprecipitation (IP) was performed with either control IgG (left lane) or G213 (right lane). Samples were resolved on an SDS-PAGE gel, followed by immunoblotting with G213. The 15-kDa species specifically recognized by G213 is indicated with an asterisk. (D) Cell lysates derived from HEK293T cell transfectants expressing either FLAG-tagged S100A9 or HA-tagged S100A8 as well as mock-treated cells were resolved on SDS-PAGE gels, and immunoblotting with Abs to FLAG, HA, and β-actin as well as G213 mAb was conducted.

    Journal: Blood Advances

    Article Title: Neutrophils and the S100A9 protein critically regulate granuloma formation

    doi: 10.1182/bloodadvances.2016000497

    Figure Lengend Snippet: Identification of the G213-reactive protein as S100A9 . (A) Tissue lysates derived from the lungs of untreated (left lane) and BCG-challenged (right lane) guinea pigs were resolved on SDS-PAGE, and western blotting was conducted with G213 (upper panel) and Ab to β-actin (lower panel). The 15-kDa species specifically recognized by G213 is indicated with an asterisk. The 30-kDa bands observed on both lanes may represent nonspecific signals. (B) Tissue lysates derived from the bone marrow, spleen, lymph nodes, and thymus of an untreated guinea pig were prepared, and western blotting was performed as in panel A. The 15-kDa species specifically recognized by G213 is indicated with an asterisk. (C) Bone marrow cells obtained from an untreated guinea pig were lysed, and immunoprecipitation (IP) was performed with either control IgG (left lane) or G213 (right lane). Samples were resolved on an SDS-PAGE gel, followed by immunoblotting with G213. The 15-kDa species specifically recognized by G213 is indicated with an asterisk. (D) Cell lysates derived from HEK293T cell transfectants expressing either FLAG-tagged S100A9 or HA-tagged S100A8 as well as mock-treated cells were resolved on SDS-PAGE gels, and immunoblotting with Abs to FLAG, HA, and β-actin as well as G213 mAb was conducted.

    Article Snippet: Sixty-micrometer-thick sections were prepared and incubated with G213 mAb, followed by the 1.4-nm gold particle–conjugated Fab′ fragment of anti-rat IgG Abs (Nanoprobes, Yaphank, NY).

    Techniques: Derivative Assay, SDS Page, Western Blot, Immunoprecipitation, Expressing

    Effect of vildagliptin on capillary vessels in isoproterenol-treated rat myocardium. CD31 staining of hearts in the vehicle, ISO, and ISO-VL groups. Scale bar = 100 μm. Bar graph shows the number of vessels per cardiomyocyte. Values represent the mean ± SE. n = 6 each. NS, not significant.

    Journal: Cardiovascular Diabetology

    Article Title: Effect of vildagliptin, a dipeptidyl peptidase 4 inhibitor, on cardiac hypertrophy induced by chronic beta-adrenergic stimulation in rats

    doi: 10.1186/1475-2840-13-43

    Figure Lengend Snippet: Effect of vildagliptin on capillary vessels in isoproterenol-treated rat myocardium. CD31 staining of hearts in the vehicle, ISO, and ISO-VL groups. Scale bar = 100 μm. Bar graph shows the number of vessels per cardiomyocyte. Values represent the mean ± SE. n = 6 each. NS, not significant.

    Article Snippet: To visualize the capillaries in the myocardium, endothelial cells in frozen sections were stained with anti-rat CD31 Ab (BD Pharmingen) at 1:100 dilution.

    Techniques: Staining

    Prdm6 affects angiogenic patterning. ( A ) Unusual clusters of densely growing vessel structures on yolk sacs of Prdm6 -deficient embryos, as observed under a stereomicroscope. Scale bars correspond to 1 mm (left panel) or 100 µm (right panel). ( B ) Large vessels in the yolk sacs of E12.5 control and Prdm6 -deficient (del/del) embryos under a stereomicroscope. Scale bars correspond to 500 µm. ( C ) Visualization of E10.5 (left panels) and E11.5 (right panels) yolk sac microvascular systems via immunofluorescent staining with an anti-CD31 primary antibody and a Cy3-conjugated secondary antibody. Scale bars correspond to 200 µm. ( D ) Quantitative morphometric analysis of the yolk sac vasculature as shown in (C). Avascular space and mean vessel diameters of yolk sacs at E10.5 – E 11.5 are shown as mean ± SEM, n=6. More details about this analysis are given in Figure S2 .

    Journal: PLoS ONE

    Article Title: Prdm6 Is Essential for Cardiovascular Development In Vivo

    doi: 10.1371/journal.pone.0081833

    Figure Lengend Snippet: Prdm6 affects angiogenic patterning. ( A ) Unusual clusters of densely growing vessel structures on yolk sacs of Prdm6 -deficient embryos, as observed under a stereomicroscope. Scale bars correspond to 1 mm (left panel) or 100 µm (right panel). ( B ) Large vessels in the yolk sacs of E12.5 control and Prdm6 -deficient (del/del) embryos under a stereomicroscope. Scale bars correspond to 500 µm. ( C ) Visualization of E10.5 (left panels) and E11.5 (right panels) yolk sac microvascular systems via immunofluorescent staining with an anti-CD31 primary antibody and a Cy3-conjugated secondary antibody. Scale bars correspond to 200 µm. ( D ) Quantitative morphometric analysis of the yolk sac vasculature as shown in (C). Avascular space and mean vessel diameters of yolk sacs at E10.5 – E 11.5 are shown as mean ± SEM, n=6. More details about this analysis are given in Figure S2 .

    Article Snippet: For anti-CD31 staining, primary rat anti-mouse CD31 Ab (BD Pharmingen, clone MEC 13.3) was used, followed by fluorescent labeling with a secondary anti-rat Cy2 Ab or anti-rat Cy3 Ab (both from Jackson Immuno Research).

    Techniques: Staining

    Immunohistochemical detection of TLR-4 expression in lung tissue, magnification ×400. ( A ) 12W CG group, ( B ) 16W CG group, ( C ) 12W IH group, ( D ) 16W IH group, ( E ) 12W HI group, and ( F ) 16W HI group. * Compared with 12W CG, P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Toll-Like Receptor 4 (TLR-4) Pathway Promotes Pulmonary Inflammation in Chronic Intermittent Hypoxia-Induced Obstructive Sleep Apnea

    doi: 10.12659/MSM.910632

    Figure Lengend Snippet: Immunohistochemical detection of TLR-4 expression in lung tissue, magnification ×400. ( A ) 12W CG group, ( B ) 16W CG group, ( C ) 12W IH group, ( D ) 16W IH group, ( E ) 12W HI group, and ( F ) 16W HI group. * Compared with 12W CG, P

    Article Snippet: The membranes were blocked with 5% BSA in TBST for 3 h at room temperature, then washed and incubated with primary anti-rat TLR-4 Abs (1/1000 dilution) (R & D Company, USA) overnight at 4°C.

    Techniques: Immunohistochemistry, Expressing

    Real-time PCR results of mRNA levels of TLR-4 ( A ) and Western blotting results of expression of TLR-4 ( B ) tissues of rats in each group; TLR-4 band density ( C ). * P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Toll-Like Receptor 4 (TLR-4) Pathway Promotes Pulmonary Inflammation in Chronic Intermittent Hypoxia-Induced Obstructive Sleep Apnea

    doi: 10.12659/MSM.910632

    Figure Lengend Snippet: Real-time PCR results of mRNA levels of TLR-4 ( A ) and Western blotting results of expression of TLR-4 ( B ) tissues of rats in each group; TLR-4 band density ( C ). * P

    Article Snippet: The membranes were blocked with 5% BSA in TBST for 3 h at room temperature, then washed and incubated with primary anti-rat TLR-4 Abs (1/1000 dilution) (R & D Company, USA) overnight at 4°C.

    Techniques: Real-time Polymerase Chain Reaction, Western Blot, Expressing

    Immunohistochemical staining for CD68. CD68 + macrophages are located in the renal interstitium indicated by white arrow, blood vessels indicated by black arrow or the lumen of the renal tubules indicated by red arrow (×400).

    Journal: PLoS ONE

    Article Title: The role of complement activation in rhabdomyolysis-induced acute kidney injury

    doi: 10.1371/journal.pone.0192361

    Figure Lengend Snippet: Immunohistochemical staining for CD68. CD68 + macrophages are located in the renal interstitium indicated by white arrow, blood vessels indicated by black arrow or the lumen of the renal tubules indicated by red arrow (×400).

    Article Snippet: The sections were then incubated with the following anti-rat antibodies overnight at 4°C: anti-rat IL-6 Ab (1:100, Santa Cruz) and anti-rat CD68 Ab (1:100, Santa Cruz).

    Techniques: Immunohistochemistry, Staining

    Dam1p is at the kinetochore. ( A ) IF of chromosome spreads from cells containing Ndc10pHA and Dam1p-myc. Blue shows DAPI (DNA), green shows Ndc10pHA detected with anti-HA mAb plus FITC anti-mouse secondary Ab, and red shows Dam1pMyc detected with polyclonal anti-Myc Ab plus Cy3 anti-rabbit secondary Ab. (Bar = 1.5 μm.) ( B ) Dam1-GFP is associated with centromeric DNA in vivo , in an NDC10 -dependent manner. CEN4 association was tested in wild-type or ndc10 - 1 cells at 37°C. Background signal was detected by using URA3 -specific oligonucleotides. Total input, DNA extract of total lysate before immunoprecipitation; IP, DNA extract after immunoprecipitation.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Yeast Dam1p has a role at the kinetochore in assembly of the mitotic spindle

    doi: 10.1073/pnas.241417098

    Figure Lengend Snippet: Dam1p is at the kinetochore. ( A ) IF of chromosome spreads from cells containing Ndc10pHA and Dam1p-myc. Blue shows DAPI (DNA), green shows Ndc10pHA detected with anti-HA mAb plus FITC anti-mouse secondary Ab, and red shows Dam1pMyc detected with polyclonal anti-Myc Ab plus Cy3 anti-rabbit secondary Ab. (Bar = 1.5 μm.) ( B ) Dam1-GFP is associated with centromeric DNA in vivo , in an NDC10 -dependent manner. CEN4 association was tested in wild-type or ndc10 - 1 cells at 37°C. Background signal was detected by using URA3 -specific oligonucleotides. Total input, DNA extract of total lysate before immunoprecipitation; IP, DNA extract after immunoprecipitation.

    Article Snippet: The following Abs were used: for visualizing microtubules, primary mAb YOL1/34 and secondary Texas red- or FITC-conjugated anti-rat Abs (Accurate Chemical and Scientific, Westbury, NY); and for visualizing Pds1-myc, Dam1p-myc, and dam1–1p-myc, we used polyclonal anti-myc Ab (kind gift of J. Yucel, Univ. of Colorado, Boulder, CO) and secondary Cy3-conjugated anti-rabbit Abs.

    Techniques: In Vivo, Immunoprecipitation