Journal: Journal of Dental Sciences

Article Title: Overexpression of sprouty 1 protein in human oral squamous cell carcinogenesis

doi: 10.1016/j.jds.2020.07.013

Figure Lengend Snippet: Western blot analyses: phosphor-ERK and total-ERK in human oral cancer cell lines as compared with a primary culture of normal oral mucosa (HOK). Upregulation of phosphor-ERK (normalized to total-ERK) in OECM1 and Ca922 cell lines as compared with HOK, and a slightly decreased expression in SAS as compared with HOK. Results were quantified using densitometric analysis, normalized to the level of β-actin, and expressed as a fold change relative to the normal oral mucosa. Bars represent means ± standard deviation of the mean (∗ P < 0.05). A representative result of three independent experiments is shown.

Article Snippet: Further, samples were analyzed using 10% SDS-PAGE (Sigma-Aldrich) gels, and the proteins were transmitted onto a PVDF membrane (Sigma-Aldrich) using Bio-Rad's transblot with primary antibodies against phosphor-ERK (Boster Biological Technology, CA, USA; Cat. No. P00104; 1:1000) and total-ERK (Boster Biological Technology; Cat. No. P00104; 1:1000), with species specificity for human tissues and an observed molecular weight of 42–44 kDa; and β-actin (Sigma-Aldrich; 1:1000), followed by horseradish peroxidase (HRP)-conjugated secondary antibodies (Sigma-Aldrich; 1:5000).

Techniques: Western Blot, Expressing, Standard Deviation

Journal: Bioscience trends

Article Title: Mongolian Medicine echinops prevented postmenopausal osteoporosis and induced ER/AKT/ERK pathway in BMSCs.

doi: 10.5582/bst.2018.01046

Figure Lengend Snippet: Figure 6. Effects of echinops-containing serum on expressions of ERα, ERβ, AKT, p-AKT, ERK, and P-ERK in BMSCs. BMSCs were treated with Echniops-containing serum that collected from rats administrated with 16.26 (low), 32.5 (middle), and 65 (high) mg/kg echinops. (A) ERα mRNA. (B) ERβ mRNA. (C) ERα, ERβ, AKT, p-AKT, ERK, and p-ERK. (D) Quantification of western bolts. *p < 0.05, **p < 0.01 vs. control.

Article Snippet: The membrane was incubated with 5% non-fat milk for 30 min, and incubated with primary antibodies ERα (1:2,000), ERβ (1:4,000), AKT (1:1,500), p-AKT (1:1,000), ERK (1:1,000), p-ERK (1:2,000), and GAPDH (1:10,000) antibody at room temperature for 1 h, and then incubated with HRP goat anti-rabbit IgG secondary antibody (1:20,000, BOSTER, China) at room temperature for 40 min.

Techniques: Western Blot, Control

Journal:

Article Title: Hyperosmotic Stress Induces Nuclear Factor-?B Activation and Interleukin-8 Production in Human Intestinal Epithelial Cells

doi:

Figure Lengend Snippet: Hyperosmolarity induces p38 and p42/44 activation in HT-29 cells (A). Cells were incubated with hyperosmotic medium prepared by the addition of mannitol (for a final concentration of 100 mmol/L) to isosmolar medium for the indicated time periods. p38 and p42/44 activation was determined using Western blotting using antibodies raised against the active, double-phosphorylated form of p38 and p42/44. This figure is representative of two separate experiments. Treatment of HT-29 cells with the selective p38 inhibitor SB203580 or selective p42/44 inhibitor PD98059 suppresses the hyperosmolarity-induced IL-8 response (B). Hyperosmolarity was achieved by the addition of mannitol (for a final concentration of 100 mmol/L) to isosmolar medium. In control wells, cells were incubated with isosmolar medium. Data are mean ± SEM of n = 12 wells from two separate experiments. *, P < 0.05; **, P < 0.01. Dotted bar, no mannitol; cross-hatched bars, mannitol.

Article Snippet: The membrane was probed with anti-phospho-p38 or anti-phospho-p42/44 MAP kinase antibody (Promega) and subsequently incubated with a secondary horseradish peroxidase-conjugated donkey anti-rabbit antibody (Boehringer).

Techniques: Activation Assay, Incubation, Concentration Assay, Western Blot