Journal: Pulmonary Circulation

Article Title: Transmembrane protein 16A/anoctamin 1 inhibitor T16A inh -A01 reversed monocrotaline-induced rat pulmonary arterial hypertension

doi: 10.1177/2045894020946670

Figure Lengend Snippet: Upregulation of TMEM16A expression in the PAs of PAH-modeling rats and its correlation with PCNA and WA%. Representative Western blots are shown. Pulmonary arterial tissues were obtained for Western blots from rats treated with MCT for 0 (control), 1 (1W), 2 (2W), 3 (3W), and 4 weeks (4W). Representative Western blot band of TMEM16A (a), PCNA (b), P-ERK1/2 (c) of pulmonary arterial tissues from rats treated with MCT for 0 (control), 1 (1W), 2 (2W), 3 (3W), and 4 weeks (4W). (d) to (f) Analytic data of TMEM16A, PCNA, P-ERK1/2 protein expression. (g) TMEM16A mRNA expression of pulmonary arterial tissues from rats treated with MCT for 0 (control), 1 (1W), 2 (2W), 3 (3W), and 4 weeks (4W). **: P < 0.01 versus control. n = 6 for each group. ERK1/2: extracellular regulated protein kinases; NS: not significant; PCNA: proliferating cell nuclear antigen; TMEM16A: transmembrane protein 16A.

Article Snippet: The proteins were separated on the gel and then transferred onto polyvinylidene difluoride membranes, which were blocked with 5% skimmed milk for 2 h and immunoblotted with a primary antibody against TMEM16A (Abcam, Cambridge, UK) at a dilution of 1:1000, a PCNA (Boster, CA, USA) at a dilution of 1:250, or an antibody against β-actin (Santa Cruz Biotechnology, Dallas, TX, US) at a dilution of 1:1000.

Techniques: Expressing, Western Blot, Control

Journal: Oncologie

Article Title: Forkhead Box P4 promotes the proliferation of cells in colorectal adenocarcinoma

doi: 10.1515/oncologie-2023-0009

Figure Lengend Snippet: Figure 2: Correlation of FOXP4 and PCNA in CRAC.

Article Snippet: Rabbit anti-humanFOXP4 (16772-1-AP)wasprocured from Wuhan proteintech (Wuhan, China), and rabbit anti-human proliferating cell nuclear antigen (PCNA) (A00125) was procured from Wuhan Boster Biological Technology (Wuhan, China).

Techniques:

Journal: Animal biotechnology

Article Title: Age-dependent changes in the expression and localization of LYZL4, LYZL6 and PCNA during testicular development in the Ashidan yak.

doi: 10.1080/10495398.2024.2344213

Figure Lengend Snippet: Figure 1. Relative expression of LYZL4, LYZL6 and PCNA mRNA in testis of the yak; different letters indicate significant differ ence (p < 0.01).

Article Snippet: The proteins were extracted from the testis following a previously established procedure.2 The protein was fixed in 12% Tricine SDS-PAGE for gel electrophoresis, then shifted onto PVDF membranes (Roche, Indianapolis, Indiana), after blocking of the membranes in 1 × 9 PBS and 0.1% Tween-20 containing 5% nonfat milk, then washed with PBS/Tween and membranes were incubated at 4°С for overnight with anti LYZL4 (1:1000, Bioss, Beijing, China), anti LYZL6 and anti PCNA antibody (1:1000, Aviva Systems Biology, San Diego, California) and anti β-actin (1:1000; Abcam, Cambridge, UK).

Techniques: Expressing

Journal: Animal biotechnology

Article Title: Age-dependent changes in the expression and localization of LYZL4, LYZL6 and PCNA during testicular development in the Ashidan yak.

doi: 10.1080/10495398.2024.2344213

Figure Lengend Snippet: Figure 2. Expression of LYZL4, LYZL6 and PCNA protein in tes tis of the yak; (A) the LYZL4, LYZL6 and PCNA detected by Western blotting; (B) The protein expression of LYZL4, LYZL6 and PCNA; different letters indicate significant difference (p < 0.05).

Article Snippet: The proteins were extracted from the testis following a previously established procedure.2 The protein was fixed in 12% Tricine SDS-PAGE for gel electrophoresis, then shifted onto PVDF membranes (Roche, Indianapolis, Indiana), after blocking of the membranes in 1 × 9 PBS and 0.1% Tween-20 containing 5% nonfat milk, then washed with PBS/Tween and membranes were incubated at 4°С for overnight with anti LYZL4 (1:1000, Bioss, Beijing, China), anti LYZL6 and anti PCNA antibody (1:1000, Aviva Systems Biology, San Diego, California) and anti β-actin (1:1000; Abcam, Cambridge, UK).

Techniques: Expressing, Western Blot