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Bio-Techne corporation
nfix antibody Nfix Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/anti-nfix/NFIX+Antibody/custom%40nbp2-15039%4032291386 Average 94 stars, based on 1 article reviews
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GenScript corporation
anti-nfix Anti Nfix, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/anti-nfix/anti+nfix/pmc06349116-528-44-50 Average 90 stars, based on 1 article reviews
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Geneka Biotechnology Inc
rabbit polyclonal antibody for ngfib Rabbit Polyclonal Antibody For Ngfib, supplied by Geneka Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more https://www.bioz.com/product/anti-nfix/rabbit+anti+nfix/10__1074_slash_jbc__m405431200-125-0-10 Average 90 stars, based on 1 article reviews
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The protein encoded by this gene is a transcription factor that binds the palindromic sequence 5'-TTGGCNNNNNGCCAA-3 in viral and cellular promoters. The encoded protein can also stimulate adenovirus replication in vitro. Three transcript variants encoding
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Boster Bio Anti-NFIX Antibody Picoband® catalog # A04138-1. Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees
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Recognizes and binds the palindromic sequence 5-TTGGCNNNNNGCCAA-3 present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.Shipped at 4°C.
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Recognizes and binds the palindromic sequence 5-TTGGCNNNNNGCCAA-3 present in viral and cellular promoters and in the origin of replication of adenovirus type 2. These proteins are individually capable of activating transcription and replication.Shipped at 4°C.
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Image Search Results
Journal: Scientific Reports
Article Title: MiR-744-5p inducing cell death by directly targeting HNRNPC and NFIX in ovarian cancer cells
doi: 10.1038/s41598-018-27438-6
Figure Lengend Snippet: miRNA correlation to potential targets on mRNA level of the TCGA ovarian serous cystadenocarcinoma dataset.
Article Snippet: For detection of target proteins the following antibodies were used: HNRNPC (1:1,000; #91327; Cell Signaling Technology),
Techniques: Binding Assay
![HNRNPC and NFIX as direct targets for miR-744. To validate miR-744 mediated regulation of potential target genes, SKOV3 and OVCAR3 cells were transfected with miR-744 mimic or non-targeting siRNA (NT, negative control for cell death) as described in Fig. . After 48 h, potential target gene expression was analysed by qRT-PCR. The relative mRNA expression of potential target genes was normalized to PPIA and NT employing the Livak method ( a ). Significantly downregulated potential targets were further analysed by the Luciferase Reporter Assay ( b and c ) to proof direct binding of the miRNA to its predicted target sequence. Anti-miR-744 was used to confirm the binding sides of miR-744 to the target mRNAs ( d ). The binding sites in the 3′UTR (Table ), based on data of microRNA.org and TargetScanHuman , were cloned into a pMirGLO vector. Relative luciferase activity was measured 72 h after co-transfection of the pMirGLO vector with miR-744 mimic, miRNA inhibitor anti-miR-744 or non-targeting siRNA (NT) in HEK293T cells. The relative luciferase expression was normalized to the expression after co-transfection with NT. For Western Blot analysis, SKOV3 and OVCAR3 cells were harvested 60 h after miR-744 transfection ( e ). The representative blots were stained with antibodies against NFIX and HNRNPC. GAPDH was used as loading control. Statistical differences were tested using paired t-test [n = 3 biological and technical replicates; mean ± SD; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001].](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8049/pmc05998049/pmc05998049__41598_2018_27438_Fig6_HTML.jpg)
Journal: Scientific Reports
Article Title: MiR-744-5p inducing cell death by directly targeting HNRNPC and NFIX in ovarian cancer cells
doi: 10.1038/s41598-018-27438-6
Figure Lengend Snippet: HNRNPC and NFIX as direct targets for miR-744. To validate miR-744 mediated regulation of potential target genes, SKOV3 and OVCAR3 cells were transfected with miR-744 mimic or non-targeting siRNA (NT, negative control for cell death) as described in Fig. . After 48 h, potential target gene expression was analysed by qRT-PCR. The relative mRNA expression of potential target genes was normalized to PPIA and NT employing the Livak method ( a ). Significantly downregulated potential targets were further analysed by the Luciferase Reporter Assay ( b and c ) to proof direct binding of the miRNA to its predicted target sequence. Anti-miR-744 was used to confirm the binding sides of miR-744 to the target mRNAs ( d ). The binding sites in the 3′UTR (Table ), based on data of microRNA.org and TargetScanHuman , were cloned into a pMirGLO vector. Relative luciferase activity was measured 72 h after co-transfection of the pMirGLO vector with miR-744 mimic, miRNA inhibitor anti-miR-744 or non-targeting siRNA (NT) in HEK293T cells. The relative luciferase expression was normalized to the expression after co-transfection with NT. For Western Blot analysis, SKOV3 and OVCAR3 cells were harvested 60 h after miR-744 transfection ( e ). The representative blots were stained with antibodies against NFIX and HNRNPC. GAPDH was used as loading control. Statistical differences were tested using paired t-test [n = 3 biological and technical replicates; mean ± SD; * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001].
Article Snippet: For detection of target proteins the following antibodies were used: HNRNPC (1:1,000; #91327; Cell Signaling Technology),
Techniques: Transfection, Negative Control, Targeted Gene Expression, Quantitative RT-PCR, Expressing, Luciferase, Reporter Assay, Binding Assay, Sequencing, Clone Assay, Plasmid Preparation, Activity Assay, Cotransfection, Western Blot, Staining, Control
Journal: Scientific Reports
Article Title: MiR-744-5p inducing cell death by directly targeting HNRNPC and NFIX in ovarian cancer cells
doi: 10.1038/s41598-018-27438-6
Figure Lengend Snippet: Predicted binding sides cloned into a pmirGlo Dual Luciferase miRNA target expression vector.
Article Snippet: For detection of target proteins the following antibodies were used: HNRNPC (1:1,000; #91327; Cell Signaling Technology),
Techniques: Binding Assay, Clone Assay, Luciferase, Expressing, Plasmid Preparation, Sequencing
![Signalling pathways leading to apoptosis. Western Blot data based on literature research , after transfection of SKOV3 cells with miRNA-744 or with siRNA against NFIX ( a ) or HNRNPC ( b ) and qRT-PCR ( c ) confirmed the potential signalling pathway by reduced expression of NFIX and Bcl2 as well as diminished phosphorylation of AKT. By binding to NFIX and HNRNPC miRNA-744 is interfering with the intrinsic apoptotic pathway. Downregulation of HNRNPC resulted in enhanced expression and activation of PDCD4 and p70S6K ( e ). Reduced NFIX expression, induced by binding of miR-744, leads to reduced expression of Bcl2 leading to apoptosis ( d ). The expression analysis of miR-744 and miR-21 was normalized to the CT value of U6 snRNA . Statistical differences were tested using paired t-test. In order to analyse DNA fragmentation (cells in sub G0/G1, d ) SKOV3 cells were seeded and transfected as described in Fig. with miR-744 mimics, non-targeting siRNA (NT, negative control for cell death), or siRNA against NFIX or HNRNPC. Statistical analysis was performed by one-way ANOVA followed by Bonferroni post-test ( d ). [n = 3 technical replicates; mean ± SD; ** p < 0.01; *** p < 0.001; **** p < 0.0001]. The cells were harvested for Western Blot analysis 60 h after treatment (a and b). GAPDH was used as loading control.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_8049/pmc05998049/pmc05998049__41598_2018_27438_Fig7_HTML.jpg)
Journal: Scientific Reports
Article Title: MiR-744-5p inducing cell death by directly targeting HNRNPC and NFIX in ovarian cancer cells
doi: 10.1038/s41598-018-27438-6
Figure Lengend Snippet: Signalling pathways leading to apoptosis. Western Blot data based on literature research , after transfection of SKOV3 cells with miRNA-744 or with siRNA against NFIX ( a ) or HNRNPC ( b ) and qRT-PCR ( c ) confirmed the potential signalling pathway by reduced expression of NFIX and Bcl2 as well as diminished phosphorylation of AKT. By binding to NFIX and HNRNPC miRNA-744 is interfering with the intrinsic apoptotic pathway. Downregulation of HNRNPC resulted in enhanced expression and activation of PDCD4 and p70S6K ( e ). Reduced NFIX expression, induced by binding of miR-744, leads to reduced expression of Bcl2 leading to apoptosis ( d ). The expression analysis of miR-744 and miR-21 was normalized to the CT value of U6 snRNA . Statistical differences were tested using paired t-test. In order to analyse DNA fragmentation (cells in sub G0/G1, d ) SKOV3 cells were seeded and transfected as described in Fig. with miR-744 mimics, non-targeting siRNA (NT, negative control for cell death), or siRNA against NFIX or HNRNPC. Statistical analysis was performed by one-way ANOVA followed by Bonferroni post-test ( d ). [n = 3 technical replicates; mean ± SD; ** p < 0.01; *** p < 0.001; **** p < 0.0001]. The cells were harvested for Western Blot analysis 60 h after treatment (a and b). GAPDH was used as loading control.
Article Snippet: For detection of target proteins the following antibodies were used: HNRNPC (1:1,000; #91327; Cell Signaling Technology),
Techniques: Western Blot, Transfection, Quantitative RT-PCR, Expressing, Phospho-proteomics, Binding Assay, Activation Assay, Negative Control, Control