anti-nfatc3 Search Results


mouse monoclonal nfatc1 7a6  (Developmental Studies Hybridoma Bank)
91
Developmental Studies Hybridoma Bank mouse monoclonal nfatc1 7a6
Sagittal sections of E10.5 mutant ( B, D, G ) and control ( A, C, F ) embryos analyzed by pHH3 and <t>NFATc1</t> immunostaining and by TUNEL assay. pHH3 immunostaining (pink) reveals similar proliferation rates in mutant and control OFT cells ( A, B ). Dotted lines show representative areas used for cell counting with ImageJ software. TUNEL assay reveals similar numbers of TUNEL-positive (green) OFT cells in mutant and control embryos ( C, D ). ( E ) Quantitative analysis of the percentage of pHH3- and TUNEL-positive OFT cells in serial sections showing a statistically insignificant trend toward less proliferation and more apoptosis in conditionally deleted embryos at E10.5. Results are expressed as mean ± SEM of the percent positive nuclei. The statistical significance of differences between groups was analyzed by the Student’s t-test. ( F, G ) Expression of the endocardial marker NFATc1 (pink), is not significantly different between conditional mutant and control embryos. Saggital sections of anti-Tfap2a immunostained Jun flox/− ( H ) and Isl1 Cre/+ ; Jun flox/− ( I ) embryos showing no significant difference in Tfap2a-expressing neural crest cells (arrowheads) at E10.5. Sections were co-stained with DAPI to illustrate nuclei. A, atrium; AVC, atrioventricular cushion; LV, left ventricle; OFT, outflow tract; OFTC, outflow tract cushion; SMA, smooth muscle actin.
Mouse Monoclonal Nfatc1 7a6, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal nfatc1 7a6/product/Developmental Studies Hybridoma Bank
Average 91 stars, based on 1 article reviews
mouse monoclonal nfatc1 7a6 - by Bioz Stars, 2026-02
91/100 stars
  Buy from Supplier

Image Search Results


Sagittal sections of E10.5 mutant ( B, D, G ) and control ( A, C, F ) embryos analyzed by pHH3 and NFATc1 immunostaining and by TUNEL assay. pHH3 immunostaining (pink) reveals similar proliferation rates in mutant and control OFT cells ( A, B ). Dotted lines show representative areas used for cell counting with ImageJ software. TUNEL assay reveals similar numbers of TUNEL-positive (green) OFT cells in mutant and control embryos ( C, D ). ( E ) Quantitative analysis of the percentage of pHH3- and TUNEL-positive OFT cells in serial sections showing a statistically insignificant trend toward less proliferation and more apoptosis in conditionally deleted embryos at E10.5. Results are expressed as mean ± SEM of the percent positive nuclei. The statistical significance of differences between groups was analyzed by the Student’s t-test. ( F, G ) Expression of the endocardial marker NFATc1 (pink), is not significantly different between conditional mutant and control embryos. Saggital sections of anti-Tfap2a immunostained Jun flox/− ( H ) and Isl1 Cre/+ ; Jun flox/− ( I ) embryos showing no significant difference in Tfap2a-expressing neural crest cells (arrowheads) at E10.5. Sections were co-stained with DAPI to illustrate nuclei. A, atrium; AVC, atrioventricular cushion; LV, left ventricle; OFT, outflow tract; OFTC, outflow tract cushion; SMA, smooth muscle actin.

Journal: PLoS ONE

Article Title: Jun Is Required in Isl1 -Expressing Progenitor Cells for Cardiovascular Development

doi: 10.1371/journal.pone.0057032

Figure Lengend Snippet: Sagittal sections of E10.5 mutant ( B, D, G ) and control ( A, C, F ) embryos analyzed by pHH3 and NFATc1 immunostaining and by TUNEL assay. pHH3 immunostaining (pink) reveals similar proliferation rates in mutant and control OFT cells ( A, B ). Dotted lines show representative areas used for cell counting with ImageJ software. TUNEL assay reveals similar numbers of TUNEL-positive (green) OFT cells in mutant and control embryos ( C, D ). ( E ) Quantitative analysis of the percentage of pHH3- and TUNEL-positive OFT cells in serial sections showing a statistically insignificant trend toward less proliferation and more apoptosis in conditionally deleted embryos at E10.5. Results are expressed as mean ± SEM of the percent positive nuclei. The statistical significance of differences between groups was analyzed by the Student’s t-test. ( F, G ) Expression of the endocardial marker NFATc1 (pink), is not significantly different between conditional mutant and control embryos. Saggital sections of anti-Tfap2a immunostained Jun flox/− ( H ) and Isl1 Cre/+ ; Jun flox/− ( I ) embryos showing no significant difference in Tfap2a-expressing neural crest cells (arrowheads) at E10.5. Sections were co-stained with DAPI to illustrate nuclei. A, atrium; AVC, atrioventricular cushion; LV, left ventricle; OFT, outflow tract; OFTC, outflow tract cushion; SMA, smooth muscle actin.

Article Snippet: Immunofluorescence (IF) and horseradish peroxidase (HRP) immunostaining were performed as described previously using rabbit monoclonal anti-Jun 60A8 (1∶100 IF; Cell Signaling Technology, Danvers, MA), rat monoclonal anti-CD31 MEC13.3 (Pecam1; 1∶100; BD Pharmingen, Franklin Lakes, NJ), mouse monoclonal anti-Isl-1 39.4D5 (1∶25 IF; Developmental Studies Hybridoma Bank, Iowa City, IA), rabbit polyclonal anti-GFP (1∶200 IF, Life Technologies), mouse monoclonal anti-SMA 1A4 (1∶200 IF, Life Technologies), rabbit polyclonal phospho-histone H3 Ser10 (1∶200 IF, Cell Signaling Technology), mouse monoclonal AP-2 alpha (Tfap2a) 3B5 (1∶4 IF, Developmental Studies Hybridoma Bank) and mouse monoclonal NFATc1 7A6 (1∶25 IF, Developmental Studies Hybridoma Bank).

Techniques: Mutagenesis, Control, Immunostaining, TUNEL Assay, Cell Counting, Software, Expressing, Marker, Staining