Journal: Nature Communications

Article Title: A method for Boolean analysis of protein interactions at a molecular level

doi: 10.1038/s41467-022-32395-w

Figure Lengend Snippet: Antibodies used for MolBoolean experiments

Article Snippet: Mouse anti E-cadherin Clone CL1170 , 5 μg/mL , Atlas Antibodies, AMAb90862, 03700.

Techniques: Concentration Assay, Transduction

Journal: Experimental and Therapeutic Medicine

Article Title: MgCl 2 and ZnCl 2 promote human umbilical vein endothelial cell migration and invasion and stimulate epithelial-mesenchymal transition via the Wnt/β-catenin pathway

doi: 10.3892/etm.2017.5144

Figure Lengend Snippet: Effects of MgCl 2 and ZnCl 2 on EMT. (A) Levels of E-cadherin, N-cadherin, vimentin and Snail mRNA were measured using reverse transcription-quantitative polymerase chain reaction and normalized to β-actin expression. (B) Levels of N-cadherin, vimentin and Snail protein were quantified by western blotting. (C) E-cadherin expression was analyzed by immunofluorescence staining. The nuclei were stained with DAPI. Scale bars, 50 µm (magnification, ×400). Data are presented as the mean ± standard deviation. **P<0.01 vs. the control. EMT, epithelial-mesenchymal transition; DAPI, 4′,6-diamidino-2-phenylindole.

Article Snippet: The membranes were blocked with 5% non-fat milk at room temperature for 1 h. Subsequently, membranes were incubated with antibodies against matrix metalloproteinase (MMP)-2 (1:400; BA0569; Wuhan Boster Biological Technology, Ltd., Wuhan, China), MMP-9 (1:400; BA0573; Wuhan Boster Biological Technology, Ltd.), vimentin (1:500; bs-8533R; BIOSS, Beijing, China), Snail (1:500; bs-1371R; BIOSS), N-cadherin (1:400; BA0673; Wuhan Boster Biological Technology, Ltd.), Wnt1 (1:200; sc-5630; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) and β-catenin (1:400; BA0426; Wuhan Boster Biological Technology, Ltd.) overnight at 4°C.

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Immunofluorescence, Staining, Standard Deviation, Control

Journal: eLife

Article Title: The recycling endosome protein Rab25 coordinates collective cell movements in the zebrafish surface epithelium

doi: 10.7554/eLife.66060

Figure Lengend Snippet:

Article Snippet: Antibody , anti-Cdh1 (Rabbit polyclonal) , AnaSpec , Cat# 55527s , IF (1:1000).

Techniques: Sequencing, Ligation, Recombinant, Plasmid Preparation