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Image Search Results
Journal: The Journal of experimental medicine
Article Title: Single-cell profiling reveals unique features of diabetogenic T cells in anti-PD-1-induced type 1 diabetes mice.
doi: 10.1084/jem.20221920
Figure Lengend Snippet: Figure 3. CD4+ T cells in the pancreas show increased proliferation in anti-PD-1-induced T1D. (A) UMAP visualization of integrated CD4+ T cells detected in the pancreas. Points represent individual cells and colors denote cluster classification as labeled. (B) UMAP visualization of integrated CD4+ T cells detected in the pancreas of each treatment group. Dots represent individual cells with the color representing the cluster classification and size corresponding to the size of the clonotype. Colors denote functional annotations that correspond to the same labels shown in A. (C) Stacked bar plots denoting the percentage of cells from each treatment group within individual clusters (left panel). Heatmap showing the number of cells detected in each cluster and treatment group (middle panel) and −log10 adjusted P values from a Fisher’s exact test (right panel). (D) Violin plots quantifying percent TIGIT+ staining within CD4+FoxP3+ Treg in the pancreas measured by flow cytometry. IgG-Spt, P = 0.0235. (E) Violin plots showing gMFI of T-bet in CD4+FoxP3−CD11ahiCD62L−Tcon in the pancreas by flow cytometry analysis. IgG-PD1, P = 0.0008; Spt-PD1, P = 0.0020. (F) Violin plots quantifying percent CD127+ staining within CD4+FoxP3−CD11ahiCD62L−Tcon in the pancreas measured by flow cytometry. IgG-Spt, P < 0.0001; Spt-PD1, P = 0.0012. (G) Violin plots showing percent BCL-2+ in CD4+FoxP3−CD11ahiCD62L−
Article Snippet: Cells were preincubated with TruStain Fc Receptor Block (anti-mouse CD16/ CD32, clone 93; BioLegend) and then labeled with extracellular antibodies from BioLegend: CD8α (53-6.7), PD-1 (RMP1-30), CD29 (HMβ 1-1; BioLegend), CD48 (HM48-1), CD94 (18d3), NKG2D (CX5), CD39 (Duha59), NKG2A (16A11), TIM-3 (RMT323), SLAMF7 (4G2), TIGIT (IG9), CX3CR1 (SA011F11), CD62L (MEL-14), CD44 (IM7),
Techniques: Labeling, Functional Assay, Staining, Flow Cytometry
Journal: Cell reports
Article Title: Chromatin Landscape Underpinning Human Dendritic Cell Heterogeneity
doi: 10.1016/j.celrep.2020.108180
Figure Lengend Snippet:
Article Snippet:
Techniques: Purification, Recombinant, Produced, SYBR Green Assay, Saline, Lysis, Electron Microscopy, Labeling, Staining, Cell Isolation, Enzyme-linked Immunosorbent Assay, Control, Antibody Labeling, Reverse Transcription, DNA Library Preparation, Generated, Microarray, Software
Journal: Molecular Systems Biology
Article Title: Molecular profiling reveals features of clinical immunity and immunosuppression in asymptomatic P. falciparum malaria
doi: 10.15252/msb.202110824
Figure Lengend Snippet:
Article Snippet:
Techniques: Control, Enzyme-linked Immunosorbent Assay, Marker, Infection, Purification, Flow Cytometry, Blocking Assay, Generated, Recombinant, Saline, Staining, Software, Cytometry