Journal: Brain, behavior, and immunity

Article Title: Intermittent cytomegalovirus infection alters neurobiological metabolism and induces cognitive deficits in mice

doi: 10.1016/j.bbi.2023.12.033

Figure Lengend Snippet: Brain cell characterization flow panel.

Article Snippet: GBP2 , CF405M , Biorbyt , orb763167 , 1:200.

Techniques:

Journal: Heliyon

Article Title: Zhilong Huoxue Tongyu capsule inhibits rabbit model of hyperlipidemia and atherosclerosis through NF-κB/NLRP3 signaling pathway

doi: 10.1016/j.heliyon.2023.e20026

Figure Lengend Snippet: Details of antibodies.

Article Snippet: NFκB , Aviva Systems Biology, USA , arp38197_p050 , WB.

Techniques:

Journal: bioRxiv

Article Title: Identification of VIMP as a gene inhibiting cytokine production in human CD4+ effector T cells

doi: 10.1101/2020.07.06.189456

Figure Lengend Snippet: Representative histogram overlay for the phosphorylation of major signaling transduction proteins downstream of the TCR signaling in Teffs, assessed by flow cytometry at different time points following PMA/Ionomycin stimulation. Before stimulation, the cells were first transfected with specific siRNA against VIMP (si_VIMP) versus non-specific siRNA (si_NS) for 1 day. No significant effects on the phosphorylation levels of MAPK (p38, ERK1/2, cJun, JNK1/2) pathways and canonical (p65, p105) or non-canonical (RELB, NFκB2) NFκB pathways during the first 120 min stimulation after siRNA knockdown in Teffs. The expression of total NFAT1 protein was also unaffected by VIMP knockdown. The numbers in x-axis indicate the geometric mean (Geomean) fluorescence intensity of the different proteins or phosphorylation sites. Data are mean± standard deviation (s.d.). The other measured targets remain no significant change ( A - G ). The P-values are determined by a two-tailed paired Student’s t test. ns or unlabeled, non-significant; *P<=0.05, **P<=0.01 and ***P<=0.001. All the graphs represent data from 2-7 independent donors.

Article Snippet: The antibodies used are the following ( Table below ): VIMP/SELS (V6639, Sigma-Aldrich) (dilution 1:200) with Goat Anti-rabbit IgG H&L Alexa Fluor® 647 (A-21245, Invitrogen) (dilution 1:200), NFAT1 FITC (611060, BD) (dilution 1:50), phospho p38 MAPK (T180/Y182) Alexa Fluor 647 (562066, BD) (dilution 1:50), Anti-Human phospho NFATC1 (pS172) mAb (MAB5640, R&D Systems) (dilution 1:400), phospho NFATC2 (NFAT1) (S326) (SAB4503945, Sigma-Aldrich) (dilution 1:800), PE-Cy7 Mouse anti-ERK1/2 (pT202/pY204) (560116, BD) (dilution 1:50), phospho JNK1/2 (T182/Y185) (dilution 1:200) (558268, BD), phospho cJun (S63) (9261S, Cell Signaling) (dilution 1:200), phospho p105 NFκB1 (S933) (4806S, Cell Signaling) (dilution 1:400), phospho p100 NFκB2 (S866/870) (4810S, Cell Signaling) (dilution 1:400), phospho p65 (S529) (558422, BD) (dilution 1:50), phosphor RelB (S552) (4999S, Cell Signaling) (dilution 1:400), Anti-Rabbit IgG H&L Alexa Fluor 647 (ab 150079, Abcam) (dilution 1:1000), APC Goat Anti-mouse IgG (minimal X-reactivity) (405308, Biolegend) (dilution 1:200).

Techniques: Phospho-proteomics, Transduction, Flow Cytometry, Transfection, Knockdown, Expressing, Fluorescence, Standard Deviation, Two Tailed Test