Journal: Biomedicines

Article Title: Electroacupuncture Modulates Programmed Cell Death 1 Ligand 1 on Peripheral and Central Nervous Systems in a Mouse Fibromyalgia Pain Model.

doi: 10.3390/biomedicines13020396

Figure Lengend Snippet: Figure 2. Changing levels of PD-1 and related molecules in the dorsal root ganglion. Western blot data with five lanes of proteins for normal, fibromyalgia (FM), FM + EA, PD-L1, and Trpv1−/−

Article Snippet: The polyvinylidene difluoride was nurtured in 5% fat-free milk in TBS-T buffer (100 mM NaCl, 10 mM Tris pH 7.5, 0.1% Tween 20) and a primary monoclonal antibody against PD-1 (1:1000, cat #: CD-279, Invitrogen, Waltham, MA, USA), TRPV1 (1:1000, cat #: ACC-030, Alomone, Jerusalem, Israel), Nav1.7 (1:1000, cat #: ASC-008, Alomone, Israel), Nav1.8 (1:1000, cat #: ASC-028, Alomone, Israel), pPI3K (1:1000, cat #: PA5-28070, Invitrogen, Waltham, MA, USA), pAkt (1:1000, cat #: 9271, Cell Signaling Technology, Danvers, MA, USA), pmTOR (1:500, cat #: 05-1592, Merck, Darmstadt, Germany), pERK1/2 (1:1000, cat #: 36-8800, Invitrogen, Waltham, MA, USA), pCREB (1:1000, cat #: 06-519, Merck, Darmstadt, Germany), pNFκB (1:1000, cat #: ab86299, Abcam, Cambridge, UK), pp38 (1:1000, cat #: 44-684G, Invitrogen, Waltham, MA, USA), and pJNK (1:1000, cat #: 44-682G, Invitrogen, Waltham, MA, USA), which has high specificity to a single epitope and low cross-reactivity, with 1% bovine serum albumin for at least 1 h at room temperature.

Techniques: Western Blot

Journal: Biomedicines

Article Title: Electroacupuncture Modulates Programmed Cell Death 1 Ligand 1 on Peripheral and Central Nervous Systems in a Mouse Fibromyalgia Pain Model.

doi: 10.3390/biomedicines13020396

Figure Lengend Snippet: Figure 3. Changing levels of PD-1 and related molecules in the spinal cord. Western blot data with five lanes of proteins for normal, fibromyalgia (FM), FM + EA, PD-L1, and Trpv1−/−groups: (A) PD-1, TRPV1, Nav1.7, and Nav1.8; (B) pPI3K, pAkt, pmTOR, and pERk; and (C) pp38, pJNK, pNF-κB, and pCREB levels that were increased in the FM group and were attenuated in 2 Hz electroacupuncture (EA), PD-L1, and Trpv1−/−mice. * Significant difference to normal. # Significant difference to fibromyalgia (FM). n = 6.

Article Snippet: The polyvinylidene difluoride was nurtured in 5% fat-free milk in TBS-T buffer (100 mM NaCl, 10 mM Tris pH 7.5, 0.1% Tween 20) and a primary monoclonal antibody against PD-1 (1:1000, cat #: CD-279, Invitrogen, Waltham, MA, USA), TRPV1 (1:1000, cat #: ACC-030, Alomone, Jerusalem, Israel), Nav1.7 (1:1000, cat #: ASC-008, Alomone, Israel), Nav1.8 (1:1000, cat #: ASC-028, Alomone, Israel), pPI3K (1:1000, cat #: PA5-28070, Invitrogen, Waltham, MA, USA), pAkt (1:1000, cat #: 9271, Cell Signaling Technology, Danvers, MA, USA), pmTOR (1:500, cat #: 05-1592, Merck, Darmstadt, Germany), pERK1/2 (1:1000, cat #: 36-8800, Invitrogen, Waltham, MA, USA), pCREB (1:1000, cat #: 06-519, Merck, Darmstadt, Germany), pNFκB (1:1000, cat #: ab86299, Abcam, Cambridge, UK), pp38 (1:1000, cat #: 44-684G, Invitrogen, Waltham, MA, USA), and pJNK (1:1000, cat #: 44-682G, Invitrogen, Waltham, MA, USA), which has high specificity to a single epitope and low cross-reactivity, with 1% bovine serum albumin for at least 1 h at room temperature.

Techniques: Western Blot

Journal: Biomedicines

Article Title: Electroacupuncture Modulates Programmed Cell Death 1 Ligand 1 on Peripheral and Central Nervous Systems in a Mouse Fibromyalgia Pain Model.

doi: 10.3390/biomedicines13020396

Figure Lengend Snippet: Figure 5. Changing levels of PD-1 and related molecules in the SSC. Western blot data with five lanes of proteins for normal, FM, FM + EA, PD-L1, and Trpv1−/−groups: (A) PD-1, TRPV1, Nav1.7, and Nav1.8; (B) pPI3K, pAkt, pmTOR, and pERk; and (C) pp38, pJNK, pNF-κB, and pCREB levels that were increased in the FM group and were attenuated in 2 Hz electroacupuncture (EA), PD-L1, and Trpv1−/−mice. * Significant difference to normal. # Significant difference to fibromyalgia (FM). n = 6.

Article Snippet: The polyvinylidene difluoride was nurtured in 5% fat-free milk in TBS-T buffer (100 mM NaCl, 10 mM Tris pH 7.5, 0.1% Tween 20) and a primary monoclonal antibody against PD-1 (1:1000, cat #: CD-279, Invitrogen, Waltham, MA, USA), TRPV1 (1:1000, cat #: ACC-030, Alomone, Jerusalem, Israel), Nav1.7 (1:1000, cat #: ASC-008, Alomone, Israel), Nav1.8 (1:1000, cat #: ASC-028, Alomone, Israel), pPI3K (1:1000, cat #: PA5-28070, Invitrogen, Waltham, MA, USA), pAkt (1:1000, cat #: 9271, Cell Signaling Technology, Danvers, MA, USA), pmTOR (1:500, cat #: 05-1592, Merck, Darmstadt, Germany), pERK1/2 (1:1000, cat #: 36-8800, Invitrogen, Waltham, MA, USA), pCREB (1:1000, cat #: 06-519, Merck, Darmstadt, Germany), pNFκB (1:1000, cat #: ab86299, Abcam, Cambridge, UK), pp38 (1:1000, cat #: 44-684G, Invitrogen, Waltham, MA, USA), and pJNK (1:1000, cat #: 44-682G, Invitrogen, Waltham, MA, USA), which has high specificity to a single epitope and low cross-reactivity, with 1% bovine serum albumin for at least 1 h at room temperature.

Techniques: Western Blot

Journal: Biomedicines

Article Title: Electroacupuncture Modulates Programmed Cell Death 1 Ligand 1 on Peripheral and Central Nervous Systems in a Mouse Fibromyalgia Pain Model.

doi: 10.3390/biomedicines13020396

Figure Lengend Snippet: Figure 4. Changing levels of PD-1 and related molecules in the thalamus. Western blot data with five lanes of proteins for normal, FM, FM + EA, PD-L1, and Trpv1−/−groups. (A) PD-1, TRPV1, Nav1.7, and Nav1.8; (B) pPI3K, pAkt, pmTOR, and pERk; and (C) pp38, pJNK, pNF-κB, and pCREB levels that were increased in the FM group and were attenuated in 2 Hz electroacupuncture (EA), PD-L1, and Trpv1−/−

Article Snippet: The polyvinylidene difluoride was nurtured in 5% fat-free milk in TBS-T buffer (100 mM NaCl, 10 mM Tris pH 7.5, 0.1% Tween 20) and a primary monoclonal antibody against PD-1 (1:1000, cat #: CD-279, Invitrogen, Waltham, MA, USA), TRPV1 (1:1000, cat #: ACC-030, Alomone, Jerusalem, Israel), Nav1.7 (1:1000, cat #: ASC-008, Alomone, Israel), Nav1.8 (1:1000, cat #: ASC-028, Alomone, Israel), pPI3K (1:1000, cat #: PA5-28070, Invitrogen, Waltham, MA, USA), pAkt (1:1000, cat #: 9271, Cell Signaling Technology, Danvers, MA, USA), pmTOR (1:500, cat #: 05-1592, Merck, Darmstadt, Germany), pERK1/2 (1:1000, cat #: 36-8800, Invitrogen, Waltham, MA, USA), pCREB (1:1000, cat #: 06-519, Merck, Darmstadt, Germany), pNFκB (1:1000, cat #: ab86299, Abcam, Cambridge, UK), pp38 (1:1000, cat #: 44-684G, Invitrogen, Waltham, MA, USA), and pJNK (1:1000, cat #: 44-682G, Invitrogen, Waltham, MA, USA), which has high specificity to a single epitope and low cross-reactivity, with 1% bovine serum albumin for at least 1 h at room temperature.

Techniques: Western Blot