Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: Details of primary and secondary antibodies are used for immunohistochemistry

Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

Techniques: Immunohistochemistry

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: Expression of MT1 and MT2 genes in rat TG, SPG and hypothalamus. ( A ) Comparison of MT1 and MT2 mRNA levels in the TG of male and female rats shows that MT1 expression is significantly higher than MT2. ( B ) In both the hypothalamus (HT) and SPG of male rats, MT1 mRNA levels are also significantly higher than MT2. Data are presented as mean ± SEM, statistical significance indicate as *** p < 0.001, ** p < 0.01, * p < 0.05, and n = 6

Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

Techniques: Expressing, Comparison, Real-time Polymerase Chain Reaction

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: MT1 and MT2 immunoreactivity in TG. ( A ) positive MT1 immunoreactivity was observed in the cytoplasm and nuclei of TG neurons (thick arrows) and in SGCs (arrowheads). ( B ) MT2 immunoreactivity was observed predominantly in the cytoplasm of neurons (thick arrows), in some cases in their nuclei (thick arrows), and in Aδ-fibers (thin arrows). Negative cells are indicated by asterisks in both ( A ) and ( B ). Blue color represents nuclei staining with DAPI. ( C ) Bar graphs show the number of MT1 and MT2 immunoreactive TG neurons in male and female rats. There was no significant difference in MT1 or MT2 protein expression between sexes. However, the number of MT1-immunoreactive cells was approximately twofold higher than that of MT2 in both males and females. Data are presented as the mean ± SEM, n = 6 and * p < 0.05

Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

Techniques: Staining, Expressing, Immunofluorescence

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: Double immunohistochemistry of MT2 with CGRP or RAMP1 in TG. ( A and D ) MT2 immunoreactivity (green fluorescence) was observed in cytoplasm of TG neurons (thick arrows), and in Aδ- fibers (arrowheads). ( B ) CGRP (red fluorescence) was expressed in small to medium-sized TG neurons (thick arrows) as well as in C-fibers (arrowhead). ( C ) MT2 co-localized with CGRP (yellow-orange) in the cytoplasm of small to medium-sized neurons (thick arrows). ( E ) RAMP1 (red fluorescence) was expressed in the cytoplasm of medium to large- sized TG neurons (thick arrow) and in Aδ-fibers (arrowhead). ( F ) The merged imaged showed that MT2 was co-localized (yellow) with RAMP1 in the cytoplasm of medium to large-sized neurons (thick arrow) as well as in the Aδ- fibers (arrowhead)

Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

Techniques: Immunohistochemistry, Fluorescence, Immunofluorescence

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: Double immunohistochemistry of MT2 and CASPR in trigeminal Aδ-fibers. ( A ) MT2 receptors (green) were found in the Aδ-fibers. ( B ) CASPR immunoreactivity (red) was used to label the paranodal regions of nodes of Ranvier. ( C ) MT2 and CASPR were co-expressed in Aδ-fibers. The blue color represents nuclei staining with DAPI

Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

Techniques: Immunohistochemistry, Staining

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: Double immunohistochemistry of MT2 with CASPR and CGRP in rat dura mater. ( A and D ) Thin MT2-immunoreactive axons (green, arrows) were observed in the rat dura mater. ( B ) These fibers were identified as Aδ-fibers based on CASPR immunoreactivity (red) showing the characteristic bowtie-shaped paranodal pattern (arrowheads). ( A - C ) Fiber bundles were often seen flanking dural blood vessels (asterisks). ( C ) MT2 and CASPR were observed to be co-expressed in the same Aδ-fibers. ( E ) CGRP immunoreactivity (red, arrowheads) was detected in c-fibers throughout the rat dura mater in a granular, pearl-like pattern. ( F ) Merged images show intertwined C- and Aδ-fibers, expressing CGRP and MT2 respectively, projecting throughout the dura mater

Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

Techniques: Immunohistochemistry, Expressing, Immunofluorescence

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: Localization of MT2 immunoreactivity in the SPG and its co-localization with VIP and PACAP. ( A , D and G ) MT2 immunoreactivity (green) was observed in the cytoplasm of neurons (thick arrows), and in fibers (arrowhead). ( B and E ) VIP immunoreactivity (red) was detected in the cytoplasm of many medium-sized neurons (thick arrows) and in a few fibers (arrowheads). ( C and F ) MT2 was co-localized with VIP in the cytoplasm of medium sized neurons (yellow-orange, thick arrows). D , E and F are higher-magnification views of images A , B and C , respectively. ( H ) PACAP immunoreactivity (red) was found in the cytoplasm of SPG neurons (thick arrows). ( I ) Double staining of MT2 and PACAP showed that MT2 was co-localized with PACAP in the cytoplasm of SPG neurons (yellow-orange, thick arrow)

Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

Techniques: Double Staining, Immunofluorescence

Journal: The Journal of Headache and Pain

Article Title: Expression of melatonin receptors in trigeminal and sphenopalatine ganglia: potential targets for primary headache disorders

doi: 10.1186/s10194-025-02215-9

Figure Lengend Snippet: MT1 and MT2 immunoreactivity in the DRG. ( A ) MT1 expression in DRG was observed in both the cytoplasm and nuclei of neurons (thick arrow) and in SGCs (arrowheads). ( B ) MT2 immunoreactivity was localized in the cytoplasm of neurons and in some cases in their nuclei (thick arrow), and in the Aδ-fibers (thin arrows). The blue color represents nuclei staining with DAPI

Article Snippet: MT2 (AMR-032) , 1:200 , Rabbit , Alomone Labs, Jerusalem, Israel , AB_2340995.

Techniques: Expressing, Staining, Immunofluorescence

Journal:

Article Title: MT2-MMP-dependent release of collagen IV NC1 domains regulates submandibular gland branching morphogenesis

doi: 10.1016/j.devcel.2009.07.016

Figure Lengend Snippet: (A) qPCR analysis of SMG gene expression at distinct developmental stages shows that MT1, MT2, and MT3 increase at E13 when branching morphogenesis begins.

Article Snippet: Primary antibodies included rabbit anti-mouse MT1, MT2, and MT3 (Triple Point Biologics, OR), rat anti-mouse perlecan, goat or rabbit anti-mouse collagen IV (both Chemicon, CA), and rat anti-mouse syndecan 1 (BD Pharmingen, CA).

Techniques: Expressing

Journal:

Article Title: MT2-MMP-dependent release of collagen IV NC1 domains regulates submandibular gland branching morphogenesis

doi: 10.1016/j.devcel.2009.07.016

Figure Lengend Snippet: (A) SMGs were cultured for 36 h with non-silencing (NS) and MT1-, MT2-, and MT3-siRNAs. MT2-siRNA has the most significant effect on epithelial morphogenesis.

Article Snippet: Primary antibodies included rabbit anti-mouse MT1, MT2, and MT3 (Triple Point Biologics, OR), rat anti-mouse perlecan, goat or rabbit anti-mouse collagen IV (both Chemicon, CA), and rat anti-mouse syndecan 1 (BD Pharmingen, CA).

Techniques: Cell Culture

Journal:

Article Title: MT2-MMP-dependent release of collagen IV NC1 domains regulates submandibular gland branching morphogenesis

doi: 10.1016/j.devcel.2009.07.016

Figure Lengend Snippet: (A) Proliferation decreases after MT2-siRNA treatment. The whole mount Ki67 immunostaining in the left hand panels are a single projection. MT2-siRNA increases intracellular collagen IV staining in the epithelium (E, arrows) and mesenchyme (M, arrowheads) in the middle panels. Images are single 2 µM confocal sections.

Article Snippet: Primary antibodies included rabbit anti-mouse MT1, MT2, and MT3 (Triple Point Biologics, OR), rat anti-mouse perlecan, goat or rabbit anti-mouse collagen IV (both Chemicon, CA), and rat anti-mouse syndecan 1 (BD Pharmingen, CA).

Techniques: Immunostaining, Staining

Journal:

Article Title: MT2-MMP-dependent release of collagen IV NC1 domains regulates submandibular gland branching morphogenesis

doi: 10.1016/j.devcel.2009.07.016

Figure Lengend Snippet: (A) SMGs were cultured with MT1- or MT2-siRNAs and NC1 domains for 36 hr. The number of end buds is in the upper graph, and qPCR analysis of MT2 expression is in the lower graph. The reduction in MT2 expression is restored by NC1 domains.

Article Snippet: Primary antibodies included rabbit anti-mouse MT1, MT2, and MT3 (Triple Point Biologics, OR), rat anti-mouse perlecan, goat or rabbit anti-mouse collagen IV (both Chemicon, CA), and rat anti-mouse syndecan 1 (BD Pharmingen, CA).

Techniques: Cell Culture, Expressing

Journal:

Article Title: MT2-MMP-dependent release of collagen IV NC1 domains regulates submandibular gland branching morphogenesis

doi: 10.1016/j.devcel.2009.07.016

Figure Lengend Snippet: (A) SMGs cultured with MT1- or MT2-siRNAs, and HB-EGF (10 ng/ml) or recMT2 (0.05 µg/mL) for 36 h.

Article Snippet: Primary antibodies included rabbit anti-mouse MT1, MT2, and MT3 (Triple Point Biologics, OR), rat anti-mouse perlecan, goat or rabbit anti-mouse collagen IV (both Chemicon, CA), and rat anti-mouse syndecan 1 (BD Pharmingen, CA).

Techniques: Cell Culture

Journal:

Article Title: MT2-MMP-dependent release of collagen IV NC1 domains regulates submandibular gland branching morphogenesis

doi: 10.1016/j.devcel.2009.07.016

Figure Lengend Snippet: Working model showing how MT2-dependent release of bioactive collagen IV NC1 domains regulates protease expression and proliferation during SMG branching morphogenesis. Epithelial MT2-mediated proteolysis of collagen IV releases NC1 domains that signal via β1 integrins and downstream AKT activation to increase MT2 and Col4a2, as well as epithelial proliferation via both FGFR and HBEGF-mediated mechanisms. HBEGF further increases MT2 expression and upregulates endogenous Hbegf, providing further stimulus for rapid SMG branching morphogenesis.

Article Snippet: Primary antibodies included rabbit anti-mouse MT1, MT2, and MT3 (Triple Point Biologics, OR), rat anti-mouse perlecan, goat or rabbit anti-mouse collagen IV (both Chemicon, CA), and rat anti-mouse syndecan 1 (BD Pharmingen, CA).

Techniques: Expressing, Activation Assay