Article Title: Acute Stress Diminishes M-current Contributing to Elevated Activity of Hypothalamic-Pituitary-Adrenal Axis
Figure Lengend Snippet: Inhibition of AMPK activity restored acute stress-induced decrease in membrane fraction of Kv7.3 subunit A and B: Gel images ( A ) and quantification of band density normalized by GAPDH ( B ) show the total protein levels of AMPK in the PVN in unstressed and stressed rats. C and D , Original gel images ( C ) and quantification ( D ) of GAPDH-normalized band density of membrane fraction of Kv7.2 and Kv7.3 in the PVN in stressed rats treated with ICV injection of vehicle and dorsomorphin (100 nmol in 10 µl aCSF). E and F , Original gel images ( E ) and quantification ( F ) of GAPDH-normalized band density of cytosolic fraction of Kv7.2 and Kv7.3 in the PVN in stressed rats treated with vehicle and dorsomorphin. The molecular weight was indicated on the right side of the gel images. In all the protein assays, 4 samples (each sample contains PVN tissues of 3 rats) were used in each group. *p
Article Snippet: The immunoblots were probed with a rabbit anti-Kv7.2 antibody (1:1000, Alomone Labs) and rabbit anti-Kv7.3 antibody (1:1000, Alomone Labs).
Techniques: Inhibition, Activity Assay, Injection, Molecular Weight