Journal: Neuro-Oncology

Article Title: Blockade of Kv1.3 channels ameliorates radiation-induced brain injury

doi: 10.1093/neuonc/not221

Figure Lengend Snippet: Genetic silencing or pharmacological blockade of Kv1.3 prevents microglial activation. (A) Kv1.3-specific siRNA significantly inhibits Kv1.3 protein expression in transfected BV2 microglia. (B) Densitometric analysis of data in (A). Mean ± SEM;

Article Snippet: The blots were laid on a shaker and incubated with polyclonal rabbit anti–Cox-2 (Cell Signaling Technology) and anti-Kv1.3 (Chemicon) in blocking solution overnight at 4°C.

Techniques: Activation Assay, Expressing, Transfection

Journal: Neuro-Oncology

Article Title: Blockade of Kv1.3 channels ameliorates radiation-induced brain injury

doi: 10.1093/neuonc/not221

Figure Lengend Snippet: Kv1.3 expression increases in microglia following irradiation. (A) Kv1.3 protein expression in primary mouse microglia determined by western blotting 4 h, 12 h, 1 d, and 2 d after radiation. (B) Densitometric analysis of western blot showing Kv1.3 vs

Article Snippet: The blots were laid on a shaker and incubated with polyclonal rabbit anti–Cox-2 (Cell Signaling Technology) and anti-Kv1.3 (Chemicon) in blocking solution overnight at 4°C.

Techniques: Expressing, Irradiation, Western Blot