Journal: Environmental Health Perspectives

Article Title: Effects of Chronic Secondhand Smoke (SHS) Exposure on Cognitive Performance and Metabolic Pathways in the Hippocampus of Wild-Type and Human Tau Mice

doi: 10.1289/EHP8428

Figure Lengend Snippet: Antibodies used for dot blot analyses.

Article Snippet: IGF1B-specific , Proteintech , 1:1,000 , Rabbit , 20215-1-AP.

Techniques: Dot Blot

Journal: BMC genomics

Article Title: Expression profiles of microRNAs from lactating and non-lactating bovine mammary glands and identification of miRNA related to lactation.

doi: 10.1186/1471-2164-13-731

Figure Lengend Snippet: Figure 9 Expression of HK2 and STAT5 mRNA in the bovine mammary gland. Expression levels of HK2 and STAT5 in lactating and non-lactating bovine mammary glands were determined using real-time PCR. Gene fold changes were calculated using the 2−ΔCt

Article Snippet: Antibodies for STAT5 and β-actin were manufactured by Boster (China), and the HK2 antibody was purchased from Santa Cruz (USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: BMC genomics

Article Title: Expression profiles of microRNAs from lactating and non-lactating bovine mammary glands and identification of miRNA related to lactation.

doi: 10.1186/1471-2164-13-731

Figure Lengend Snippet: Figure 8 Putative miRNAs targeting gene 3’UTRs. (A) Pairing of bta-miR-141 with the bovine STAT5 3’UTR sequence. (B) A target seed region of the miRNA binding site was found within the bovine HK2 3’UTR sequence.

Article Snippet: Antibodies for STAT5 and β-actin were manufactured by Boster (China), and the HK2 antibody was purchased from Santa Cruz (USA).

Techniques: Sequencing, Binding Assay

Journal: BMC genomics

Article Title: Expression profiles of microRNAs from lactating and non-lactating bovine mammary glands and identification of miRNA related to lactation.

doi: 10.1186/1471-2164-13-731

Figure Lengend Snippet: Figure 10 STAT5 protein is regulated by miR-141. STAT5 and β-actin protein expression was determined by western blotting. Mac-T cells were transfected with an miR-141 inhibitor, an miR-141 mimic, an inhibitor negative control (INC) and a negative control (NC). Cell lysates were collected after 24 hours and analyzed by western blotting. Beta-actin served as the internal control. The protein fragment intensity was quantified using Imagpro-Plus software, and IOD was used to represent the protein level. Error bars represent SD. n=3. a-c means different superscripts differ (P<0.05).

Article Snippet: Antibodies for STAT5 and β-actin were manufactured by Boster (China), and the HK2 antibody was purchased from Santa Cruz (USA).

Techniques: Expressing, Western Blot, Transfection, Negative Control, Control, Software