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R&D Systems
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Boster Bio
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ABclonal Biotechnology
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Nanjing Jiancheng Bioengineering Research Institute Co Ltd
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LabForce AG
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Boster Bio
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Image Search Results
Journal: Cell Proliferation
Article Title: Oestrogen retains human periodontal ligament stem cells stemness in long‐term culture
doi: 10.1111/cpr.12396
Figure Lengend Snippet: Oestrogen enhanced osteogenic and adipogenic differentiation of hPDLSC s in long‐term culture. The osteogenic differentiation capability of hPDLSC s was tested by Alizarin red staining and quantification (A). Alkaline phosphatase (ALP) staining and ALP activity results were shown in the graph (B). The expression of proteins ( RUNX 2, BMP 2 and BSP ) was detected by Western blotting, and the relative protein expression level was normalized to β‐actin (C). The adipogenic differentiation capability of hPDLSC s was tested by oil red O staining and quantification (D). RUNX 2: Runt‐related transcription factor 2; BMP 2: Bone morphogenic protein‐2; BSP : Bone sialoprotein. Data are shown as the mean ± SD s of 3 separate experiments. * P <.05; ** P <.01
Article Snippet: After transfer, the membranes were blocked with PBS containing 5% non‐fat milk for 1 hour at room temperature, and then incubated at 4°C overnight with primary antibodies against Total AKT (T‐AKT, Boster, Wuhan, China), Phospho‐AKT (P‐AKT; Cell Signaling Technology), T‐GSK‐3β (Cell Signaling Technology), Phospho‐GSK‐3β (Cell Signaling Technology), β‐catenin (Cell Signaling Technology), Cyclin D1 (Abcam, Cambridge, MA, USA), Runt‐related transcription factor 2 (RUNX2; Boster), Bone morphogenic protein‐2 (BMP2; Boster) and
Techniques: Staining, Activity Assay, Expressing, Western Blot
Journal: Bioengineered
Article Title: The molecular mechanism research of cartilage calcification induced by osteoarthritis
doi: 10.1080/21655979.2022.2078025
Figure Lengend Snippet: Western blot analysis of expression levels of proteins DLX5, ALPL, IBSP, and ENPP1. **P < 0.01, ***P < 0.001 compared with the OA model.
Article Snippet: Pentobarbital sodium, DLX5 protein, and alizarin calcium staining kit were purchased from sigma (USA); ethylenediam ine tetraacetic acid (EDTA), PBS, FBS, and DMEM were purchased from Gibco (Shanghai, China), DLX5,
Techniques: Western Blot, Expressing