anti girk2 kir3 2 antibody Search Results


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    Alomone Labs girk2
    <t>GIRK2</t> is not regulated by Gα i3 in whole oocytes
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    GIRK2 is not regulated by Gα i3 in whole oocytes

    Journal:

    Article Title: Divergent regulation of GIRK1 and GIRK2 subunits of the neuronal G protein gated K+ channel by G?iGDP and G??

    doi: 10.1113/jphysiol.2009.173229

    Figure Lengend Snippet: GIRK2 is not regulated by Gα i3 in whole oocytes

    Article Snippet: GIRK2 expression was very sensitive to Gαi3 and Gβγ: coexpression of Gαi3 -wt and Gαi3 GA reduced PM levels of GIRK2 by up to 80%, while Gαi3 QL had a mild effect ( and Supplemental ).

    Techniques:

    Functional differences between homomeric GIRK1 and GIRK2

    Journal:

    Article Title: Divergent regulation of GIRK1 and GIRK2 subunits of the neuronal G protein gated K+ channel by G?iGDP and G??

    doi: 10.1113/jphysiol.2009.173229

    Figure Lengend Snippet: Functional differences between homomeric GIRK1 and GIRK2

    Article Snippet: GIRK2 expression was very sensitive to Gαi3 and Gβγ: coexpression of Gαi3 -wt and Gαi3 GA reduced PM levels of GIRK2 by up to 80%, while Gαi3 QL had a mild effect ( and Supplemental ).

    Techniques: Functional Assay

    Gα i3 GA does not regulate GIRK2 in excised plasma membrane patches

    Journal:

    Article Title: Divergent regulation of GIRK1 and GIRK2 subunits of the neuronal G protein gated K+ channel by G?iGDP and G??

    doi: 10.1113/jphysiol.2009.173229

    Figure Lengend Snippet: Gα i3 GA does not regulate GIRK2 in excised plasma membrane patches

    Article Snippet: GIRK2 expression was very sensitive to Gαi3 and Gβγ: coexpression of Gαi3 -wt and Gαi3 GA reduced PM levels of GIRK2 by up to 80%, while Gαi3 QL had a mild effect ( and Supplemental ).

    Techniques:

    Biochemical and functional differences between GIRK1 and GIRK2

    Journal:

    Article Title: Divergent regulation of GIRK1 and GIRK2 subunits of the neuronal G protein gated K+ channel by G?iGDP and G??

    doi: 10.1113/jphysiol.2009.173229

    Figure Lengend Snippet: Biochemical and functional differences between GIRK1 and GIRK2

    Article Snippet: GIRK2 expression was very sensitive to Gαi3 and Gβγ: coexpression of Gαi3 -wt and Gαi3 GA reduced PM levels of GIRK2 by up to 80%, while Gαi3 QL had a mild effect ( and Supplemental ).

    Techniques: Functional Assay

    The basal activity is Gβγ dependent in GIRK1*, but Gβγ independent in GIRK2

    Journal:

    Article Title: Divergent regulation of GIRK1 and GIRK2 subunits of the neuronal G protein gated K+ channel by G?iGDP and G??

    doi: 10.1113/jphysiol.2009.173229

    Figure Lengend Snippet: The basal activity is Gβγ dependent in GIRK1*, but Gβγ independent in GIRK2

    Article Snippet: GIRK2 expression was very sensitive to Gαi3 and Gβγ: coexpression of Gαi3 -wt and Gαi3 GA reduced PM levels of GIRK2 by up to 80%, while Gαi3 QL had a mild effect ( and Supplemental ).

    Techniques: Activity Assay

    GIRK subunit proteins are found in the mouse hippocampus. A , Representative immunoblots of hippocampal membrane protein samples from WT, GIRK1 KO (1), GIRK2 (2), GIRK3 (3), and GIRK2/GIRK3 (2/3) KO mice. Blots were probed with antibodies (Ab) for GIRK1, GIRK2, GIRK3, and GABA B(1a/b) . GABA B ), GIRK1 immunoreactivity was visualized as three bands, the lower molecular weight versions thought to represent core (c) and core-glycosylated (g) species. Reductions in the level of the heavily glycosylated (h) GIRK1 species were correlated with the absence of GIRK2 and GIRK3, respectively. The levels of GIRK2 and GIRK3 were also lower in samples from KO mice. B , Densitometric analysis of the impact of GIRK subunit ablation on residual GIRK protein levels in the hippocampus. Hippocampal protein samples were obtained from three separate and complete panels of WT and GIRK KO mice, and the levels of GIRK1 (heavily glycosylated form, h-GIRK1), GIRK2, GIRK3, and GABA B(1) (both isoforms) were determined. There was no effect of GIRK subunit ablation GABA B(1) receptor levels ( F (4,10) = 1.764; p = 0.213) (data not shown). * p

    Journal: The Journal of Neuroscience

    Article Title: Molecular and Cellular Diversity of Neuronal G-Protein-Gated Potassium Channels

    doi: 10.1523/JNEUROSCI.3484-05.2005

    Figure Lengend Snippet: GIRK subunit proteins are found in the mouse hippocampus. A , Representative immunoblots of hippocampal membrane protein samples from WT, GIRK1 KO (1), GIRK2 (2), GIRK3 (3), and GIRK2/GIRK3 (2/3) KO mice. Blots were probed with antibodies (Ab) for GIRK1, GIRK2, GIRK3, and GABA B(1a/b) . GABA B ), GIRK1 immunoreactivity was visualized as three bands, the lower molecular weight versions thought to represent core (c) and core-glycosylated (g) species. Reductions in the level of the heavily glycosylated (h) GIRK1 species were correlated with the absence of GIRK2 and GIRK3, respectively. The levels of GIRK2 and GIRK3 were also lower in samples from KO mice. B , Densitometric analysis of the impact of GIRK subunit ablation on residual GIRK protein levels in the hippocampus. Hippocampal protein samples were obtained from three separate and complete panels of WT and GIRK KO mice, and the levels of GIRK1 (heavily glycosylated form, h-GIRK1), GIRK2, GIRK3, and GABA B(1) (both isoforms) were determined. There was no effect of GIRK subunit ablation GABA B(1) receptor levels ( F (4,10) = 1.764; p = 0.213) (data not shown). * p

    Article Snippet: Rabbit anti-GIRK1, anti-GIRK2, and anti-GIRK3 antibodies were obtained from Alomone Labs (Jerusalem, Israel).

    Techniques: Western Blot, Mouse Assay, Molecular Weight

    Subcellular distributions of GIRK subunits in the hippocampus. Electron micrographs show immunolabeling for GIRK1 and GIRK2 in the stratum radiatum of the CA1 area of WT and GIRK KO mice. den, Dendritic shaft; b, bouton; s, dendritic spine. A , B , GIRK1 immunoparticles were observed primarily along the extrasynaptic plasma membrane (arrows) of dendritic spines of CA1 neurons from WT mice, although perisynaptic labeling (crossed arrow) was also frequently observed. GIRK1 labeling was also found associated with ER cisterna of dendritic shafts and with the spine apparatus (double arrowheads) of spines. C , GIRK1 labeling was never detected within the postsynaptic specialization, as demonstrated with postembedding techniques. D , E , GIRK1 immunoparticles at presynaptic sites were localized to the extrasynaptic plasma membrane (arrowheads) and occasionally to the presynaptic membrane specialization of axon terminals establishing putative excitatory synapses on spines, as confirmed in double-labeling experiments with VGluT1. F-H , GIRK2 immunoparticles were found along the extrasynaptic plasma membrane (arrows) of dendritic shafts of CA1 cells from WT mice, mainly associated with dendritic spines. Both perisynaptic ( G , crossed arrows) and synaptic ( H , double arrow) labeling at asymmetrical synapses was detected, the latter confirmed using postembedding techniques ( I ). J , K , GIRK2 immunoparticles were detected at presynaptic sites (arrowheads), mainly in the extra synaptic plasma membrane, and occasionally in the presynaptic membrane specialization of axon terminals establishing putative excitatory synapses on spines, as confirmed using double-labeling experiments with VGluT1. Note also the GIRK2 immunoreactivity associated with ER cisterna of dendritic shafts and the spine apparatus of dendritic spines ( J , double arrowheads). L , Distribution of GIRK1 and GIRK2 on dendritic spines of CA1 neurons. Data are displayed as percentage frequency of particles in 60-nm-wide bins, starting at the edge of the postsynaptic specialization. M-T , Electron micrographs showing GIRK1 immunoreactivity in the stratum radiatum of the CA1 area in sections taken from GIRK2 KO ( M-P ), GIRK3 KO ( Q , R ), GIRK2/GIRK3 KO ( S ), and GIRK1 KO ( T ) mice. Note that GIRK1 immunoreactivity was still observed along the extrasynaptic plasma membrane (arrows) of dendritic spines and shafts of CA1 neurons from GIRK2 KO and GIRK3 KO mice. Immunoparticles for GIRK1 were also observed at perisynaptic (data not shown) positions and at presynaptic sites along the extrasynaptic plasma membrane (arrowheads) of axon terminals establishing putative excitatory synapses on dendritic spines. A higher proportion of immunoparticles for GIRK1 was detected in the ER cisterna of dendritic shafts and the spine apparatus (double arrowhead). Immunoreactivity for GIRK1 was primarily restricted to the ER of CA1 cells in the hippocampus of GIRK2/GIRK3 KO mice and was absent in sections from a GIRK1 KO mouse. Scale bars, 0.2 μm.

    Journal: The Journal of Neuroscience

    Article Title: Molecular and Cellular Diversity of Neuronal G-Protein-Gated Potassium Channels

    doi: 10.1523/JNEUROSCI.3484-05.2005

    Figure Lengend Snippet: Subcellular distributions of GIRK subunits in the hippocampus. Electron micrographs show immunolabeling for GIRK1 and GIRK2 in the stratum radiatum of the CA1 area of WT and GIRK KO mice. den, Dendritic shaft; b, bouton; s, dendritic spine. A , B , GIRK1 immunoparticles were observed primarily along the extrasynaptic plasma membrane (arrows) of dendritic spines of CA1 neurons from WT mice, although perisynaptic labeling (crossed arrow) was also frequently observed. GIRK1 labeling was also found associated with ER cisterna of dendritic shafts and with the spine apparatus (double arrowheads) of spines. C , GIRK1 labeling was never detected within the postsynaptic specialization, as demonstrated with postembedding techniques. D , E , GIRK1 immunoparticles at presynaptic sites were localized to the extrasynaptic plasma membrane (arrowheads) and occasionally to the presynaptic membrane specialization of axon terminals establishing putative excitatory synapses on spines, as confirmed in double-labeling experiments with VGluT1. F-H , GIRK2 immunoparticles were found along the extrasynaptic plasma membrane (arrows) of dendritic shafts of CA1 cells from WT mice, mainly associated with dendritic spines. Both perisynaptic ( G , crossed arrows) and synaptic ( H , double arrow) labeling at asymmetrical synapses was detected, the latter confirmed using postembedding techniques ( I ). J , K , GIRK2 immunoparticles were detected at presynaptic sites (arrowheads), mainly in the extra synaptic plasma membrane, and occasionally in the presynaptic membrane specialization of axon terminals establishing putative excitatory synapses on spines, as confirmed using double-labeling experiments with VGluT1. Note also the GIRK2 immunoreactivity associated with ER cisterna of dendritic shafts and the spine apparatus of dendritic spines ( J , double arrowheads). L , Distribution of GIRK1 and GIRK2 on dendritic spines of CA1 neurons. Data are displayed as percentage frequency of particles in 60-nm-wide bins, starting at the edge of the postsynaptic specialization. M-T , Electron micrographs showing GIRK1 immunoreactivity in the stratum radiatum of the CA1 area in sections taken from GIRK2 KO ( M-P ), GIRK3 KO ( Q , R ), GIRK2/GIRK3 KO ( S ), and GIRK1 KO ( T ) mice. Note that GIRK1 immunoreactivity was still observed along the extrasynaptic plasma membrane (arrows) of dendritic spines and shafts of CA1 neurons from GIRK2 KO and GIRK3 KO mice. Immunoparticles for GIRK1 were also observed at perisynaptic (data not shown) positions and at presynaptic sites along the extrasynaptic plasma membrane (arrowheads) of axon terminals establishing putative excitatory synapses on dendritic spines. A higher proportion of immunoparticles for GIRK1 was detected in the ER cisterna of dendritic shafts and the spine apparatus (double arrowhead). Immunoreactivity for GIRK1 was primarily restricted to the ER of CA1 cells in the hippocampus of GIRK2/GIRK3 KO mice and was absent in sections from a GIRK1 KO mouse. Scale bars, 0.2 μm.

    Article Snippet: Rabbit anti-GIRK1, anti-GIRK2, and anti-GIRK3 antibodies were obtained from Alomone Labs (Jerusalem, Israel).

    Techniques: Immunolabeling, Mouse Assay, Labeling

    Distribution of GIRK2 in the SNc. A , In WT sections containing the SN, GIRK2 immunoreactivity was most prominent in SNc neuron dendrites found in the SNr. B , No GIRK2 staining was observed in sections from GIRK2 KO mice. C , Colocalization of the GIRK2 subunit and TH in the SNc of WT mice, as revealed using preembedding methods. The peroxidase reaction product (TH immunoreactivity) filled somata and dendritic shafts, whereas immunoparticles (GIRK2 immunoreactivity) were located along the extrasynaptic plasma membrane (arrows). D , Immunoparticles for GIRK2 (arrowheads) were also detected along the extrasynaptic plasma membrane of dendritic shafts immunonegative for TH, likely belonging to GABAergic neurons. E , F , Electron micrographs showing colocalization of the GIRK2 and GABA B(1) in the SNc of WT mice. A peroxidase reaction product (GABA B(1) immunoreactivity) filled dendritic shafts establishing asymmetrical synapses with axon terminals, whereas immunoparticles (GIRK2 immunoreactivity) were located along the extrasynaptic plasma membrane (arrows). b, Bouton; den, dendritic shaft; nuc, nucleus. Scale bars: A , B , 200 μm; C-F , 0.2 μm.

    Journal: The Journal of Neuroscience

    Article Title: Molecular and Cellular Diversity of Neuronal G-Protein-Gated Potassium Channels

    doi: 10.1523/JNEUROSCI.3484-05.2005

    Figure Lengend Snippet: Distribution of GIRK2 in the SNc. A , In WT sections containing the SN, GIRK2 immunoreactivity was most prominent in SNc neuron dendrites found in the SNr. B , No GIRK2 staining was observed in sections from GIRK2 KO mice. C , Colocalization of the GIRK2 subunit and TH in the SNc of WT mice, as revealed using preembedding methods. The peroxidase reaction product (TH immunoreactivity) filled somata and dendritic shafts, whereas immunoparticles (GIRK2 immunoreactivity) were located along the extrasynaptic plasma membrane (arrows). D , Immunoparticles for GIRK2 (arrowheads) were also detected along the extrasynaptic plasma membrane of dendritic shafts immunonegative for TH, likely belonging to GABAergic neurons. E , F , Electron micrographs showing colocalization of the GIRK2 and GABA B(1) in the SNc of WT mice. A peroxidase reaction product (GABA B(1) immunoreactivity) filled dendritic shafts establishing asymmetrical synapses with axon terminals, whereas immunoparticles (GIRK2 immunoreactivity) were located along the extrasynaptic plasma membrane (arrows). b, Bouton; den, dendritic shaft; nuc, nucleus. Scale bars: A , B , 200 μm; C-F , 0.2 μm.

    Article Snippet: Rabbit anti-GIRK1, anti-GIRK2, and anti-GIRK3 antibodies were obtained from Alomone Labs (Jerusalem, Israel).

    Techniques: Staining, Mouse Assay

    GIRK subunit mRNA distribution in the hippocampus and substantia nigra. GIRK mRNA distributions were evaluated by in situ hybridization in sections from WT and GIRKKO mice. In sections from WT mice, mRNAs for GIRK1 ( A ), GIRK2 ( B ), and GIRK3 ( C ) were clearly evident in CA1 and CA3 pyramidal neurons, as well as granule cells of the dentate gyrus (DG). There was no specific staining for GIRK mRNAs in sections from the appropriate GIRK KO mouse (data not shown). Images are representative of data obtained from three different WT and GIRK KO panels. The WT mRNA distributions of GIRK1 ( D ), GIRK2 ( E ), and GIRK3 ( F ) were also examined in the SN. Scale bars: A-C , 500 μm; D-F , 100 μm.

    Journal: The Journal of Neuroscience

    Article Title: Molecular and Cellular Diversity of Neuronal G-Protein-Gated Potassium Channels

    doi: 10.1523/JNEUROSCI.3484-05.2005

    Figure Lengend Snippet: GIRK subunit mRNA distribution in the hippocampus and substantia nigra. GIRK mRNA distributions were evaluated by in situ hybridization in sections from WT and GIRKKO mice. In sections from WT mice, mRNAs for GIRK1 ( A ), GIRK2 ( B ), and GIRK3 ( C ) were clearly evident in CA1 and CA3 pyramidal neurons, as well as granule cells of the dentate gyrus (DG). There was no specific staining for GIRK mRNAs in sections from the appropriate GIRK KO mouse (data not shown). Images are representative of data obtained from three different WT and GIRK KO panels. The WT mRNA distributions of GIRK1 ( D ), GIRK2 ( E ), and GIRK3 ( F ) were also examined in the SN. Scale bars: A-C , 500 μm; D-F , 100 μm.

    Article Snippet: Rabbit anti-GIRK1, anti-GIRK2, and anti-GIRK3 antibodies were obtained from Alomone Labs (Jerusalem, Israel).

    Techniques: In Situ Hybridization, Mouse Assay, Staining