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L1CAM mRNA and protein levels in endometrial carcinoma cell lines overexpresssing transcription <t>factors</t> <t>β-catenin</t> or Slug . ( A ) Regulation of L1CAM expression after transfection of a point mutated, constitutively active form of β-catenin (S33Y) or control pcDNA3 plasmid as determined by qRT-PCR using specific primers. ( B ) Regulation of L1CAM levels after overexpression of a HA-SLUG or control plasmid. Means ± SD from n = 3 independent experiments are shown. ( C ) Western blot analysis for L1CAM and β-catenin as well as <t>GAP-DH,</t> used as a loading control. ( D ) Western blot analysis for L1CAM and anti SLUG as well as GAP-DH, used as a loading control. A representative experiment from n = 3 is shown.
Gap Dh, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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L1CAM mRNA and protein levels in endometrial carcinoma cell lines overexpresssing transcription <t>factors</t> <t>β-catenin</t> or Slug . ( A ) Regulation of L1CAM expression after transfection of a point mutated, constitutively active form of β-catenin (S33Y) or control pcDNA3 plasmid as determined by qRT-PCR using specific primers. ( B ) Regulation of L1CAM levels after overexpression of a HA-SLUG or control plasmid. Means ± SD from n = 3 independent experiments are shown. ( C ) Western blot analysis for L1CAM and β-catenin as well as <t>GAP-DH,</t> used as a loading control. ( D ) Western blot analysis for L1CAM and anti SLUG as well as GAP-DH, used as a loading control. A representative experiment from n = 3 is shown.
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Santa Cruz Biotechnology primary antibodies
L1CAM mRNA and protein levels in endometrial carcinoma cell lines overexpresssing transcription <t>factors</t> <t>β-catenin</t> or Slug . ( A ) Regulation of L1CAM expression after transfection of a point mutated, constitutively active form of β-catenin (S33Y) or control pcDNA3 plasmid as determined by qRT-PCR using specific primers. ( B ) Regulation of L1CAM levels after overexpression of a HA-SLUG or control plasmid. Means ± SD from n = 3 independent experiments are shown. ( C ) Western blot analysis for L1CAM and β-catenin as well as <t>GAP-DH,</t> used as a loading control. ( D ) Western blot analysis for L1CAM and anti SLUG as well as GAP-DH, used as a loading control. A representative experiment from n = 3 is shown.
Primary Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Merck & Co mouse α gap dh
L1CAM mRNA and protein levels in endometrial carcinoma cell lines overexpresssing transcription <t>factors</t> <t>β-catenin</t> or Slug . ( A ) Regulation of L1CAM expression after transfection of a point mutated, constitutively active form of β-catenin (S33Y) or control pcDNA3 plasmid as determined by qRT-PCR using specific primers. ( B ) Regulation of L1CAM levels after overexpression of a HA-SLUG or control plasmid. Means ± SD from n = 3 independent experiments are shown. ( C ) Western blot analysis for L1CAM and β-catenin as well as <t>GAP-DH,</t> used as a loading control. ( D ) Western blot analysis for L1CAM and anti SLUG as well as GAP-DH, used as a loading control. A representative experiment from n = 3 is shown.
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Cell Signaling Technology Inc rabbit cell signaling 3033 anti gap dh
L1CAM mRNA and protein levels in endometrial carcinoma cell lines overexpresssing transcription <t>factors</t> <t>β-catenin</t> or Slug . ( A ) Regulation of L1CAM expression after transfection of a point mutated, constitutively active form of β-catenin (S33Y) or control pcDNA3 plasmid as determined by qRT-PCR using specific primers. ( B ) Regulation of L1CAM levels after overexpression of a HA-SLUG or control plasmid. Means ± SD from n = 3 independent experiments are shown. ( C ) Western blot analysis for L1CAM and β-catenin as well as <t>GAP-DH,</t> used as a loading control. ( D ) Western blot analysis for L1CAM and anti SLUG as well as GAP-DH, used as a loading control. A representative experiment from n = 3 is shown.
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L1CAM mRNA and protein levels in endometrial carcinoma cell lines overexpresssing transcription <t>factors</t> <t>β-catenin</t> or Slug . ( A ) Regulation of L1CAM expression after transfection of a point mutated, constitutively active form of β-catenin (S33Y) or control pcDNA3 plasmid as determined by qRT-PCR using specific primers. ( B ) Regulation of L1CAM levels after overexpression of a HA-SLUG or control plasmid. Means ± SD from n = 3 independent experiments are shown. ( C ) Western blot analysis for L1CAM and β-catenin as well as <t>GAP-DH,</t> used as a loading control. ( D ) Western blot analysis for L1CAM and anti SLUG as well as GAP-DH, used as a loading control. A representative experiment from n = 3 is shown.
Gap Dh, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc rabbit anti glyceraldehyde 3 phosphate dehydrogenase
L1CAM mRNA and protein levels in endometrial carcinoma cell lines overexpresssing transcription <t>factors</t> <t>β-catenin</t> or Slug . ( A ) Regulation of L1CAM expression after transfection of a point mutated, constitutively active form of β-catenin (S33Y) or control pcDNA3 plasmid as determined by qRT-PCR using specific primers. ( B ) Regulation of L1CAM levels after overexpression of a HA-SLUG or control plasmid. Means ± SD from n = 3 independent experiments are shown. ( C ) Western blot analysis for L1CAM and β-catenin as well as <t>GAP-DH,</t> used as a loading control. ( D ) Western blot analysis for L1CAM and anti SLUG as well as GAP-DH, used as a loading control. A representative experiment from n = 3 is shown.
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The GAPDH Antibody 1D4 DyLight 755 from Novus Biologicals is a mouse monoclonal antibody to GAPDH This antibody reacts with human mouse rat porcine avian bovine chicken equine hamster opossum primate sheep zebrafish The GAPDH
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The GAPDH Antibody NB615 DyLight 488 from Novus Biologicals is a mouse monoclonal antibody to GAPDH This antibody reacts with human mouse rat porcine bovine canine chicken rabbit The GAPDH Antibody NB615 DyLight 488 has
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The GAPDH Antibody 2D4A7 DyLight 755 from Novus Biologicals is a mouse monoclonal antibody to GAPDH This antibody reacts with human mouse rat porcine rabbit The GAPDH Antibody 2D4A7 DyLight 755 has been validated for
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Mouse monoclonal GAPDH antibody
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Image Search Results


L1CAM mRNA and protein levels in endometrial carcinoma cell lines overexpresssing transcription factors β-catenin or Slug . ( A ) Regulation of L1CAM expression after transfection of a point mutated, constitutively active form of β-catenin (S33Y) or control pcDNA3 plasmid as determined by qRT-PCR using specific primers. ( B ) Regulation of L1CAM levels after overexpression of a HA-SLUG or control plasmid. Means ± SD from n = 3 independent experiments are shown. ( C ) Western blot analysis for L1CAM and β-catenin as well as GAP-DH, used as a loading control. ( D ) Western blot analysis for L1CAM and anti SLUG as well as GAP-DH, used as a loading control. A representative experiment from n = 3 is shown.

Journal: BMC Molecular Biology

Article Title: L1CAM expression in endometrial carcinomas is regulated by usage of two different promoter regions

doi: 10.1186/1471-2199-11-64

Figure Lengend Snippet: L1CAM mRNA and protein levels in endometrial carcinoma cell lines overexpresssing transcription factors β-catenin or Slug . ( A ) Regulation of L1CAM expression after transfection of a point mutated, constitutively active form of β-catenin (S33Y) or control pcDNA3 plasmid as determined by qRT-PCR using specific primers. ( B ) Regulation of L1CAM levels after overexpression of a HA-SLUG or control plasmid. Means ± SD from n = 3 independent experiments are shown. ( C ) Western blot analysis for L1CAM and β-catenin as well as GAP-DH, used as a loading control. ( D ) Western blot analysis for L1CAM and anti SLUG as well as GAP-DH, used as a loading control. A representative experiment from n = 3 is shown.

Article Snippet: Antibodies for detection in Western blot against β-catenin were from Sigma-Aldrich (Schnelldorf, Germany) and against GAP-DH from SantaCruz Biotechnology (Heidelberg, Germany).

Techniques: Expressing, Transfection, Control, Plasmid Preparation, Quantitative RT-PCR, Over Expression, Western Blot