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  • 94
    OriGene oti4c5 mouse mab
    Oti4c5 Mouse Mab, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Proteintech flag monoclonal antibody
    Flag Monoclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Rockland Immunochemicals flag
    NbAELP of agroinjected leaves was revealed mainly in the cell wall fraction. (A) Western blot analysis with NbAELP-specific antibodies of subcellular fractions of N. benthamiana leaves at 3 day after agroinjection with A. tumefaciens containing “empty” vector (pBin19). AEP, porcine kidney mutarotase “Calzyme” (~400 ng). CW, cell wall fraction; P1, fraction enriched with nuclear proteins; P30, fraction containing mainly membrane proteins from Golgi apparatus and endoplasmic reticulum. (B,C) Western blot analysis using <t>anti-FLAG</t> antibodies of N. benthamiana leaf proteins at 3 days after agroinjection with the 35S- NbAELP:3xFLAG vector. Cell homogenate was fractionated into CW, P1, P30, S30 (fraction of soluble cytoplasmic proteins) (B) and apoplast (C) fractions. The lower panels show the protein loading control stained with Amido Black.
    Flag, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    OriGene dylight 488 ddk mab
    NbAELP of agroinjected leaves was revealed mainly in the cell wall fraction. (A) Western blot analysis with NbAELP-specific antibodies of subcellular fractions of N. benthamiana leaves at 3 day after agroinjection with A. tumefaciens containing “empty” vector (pBin19). AEP, porcine kidney mutarotase “Calzyme” (~400 ng). CW, cell wall fraction; P1, fraction enriched with nuclear proteins; P30, fraction containing mainly membrane proteins from Golgi apparatus and endoplasmic reticulum. (B,C) Western blot analysis using <t>anti-FLAG</t> antibodies of N. benthamiana leaf proteins at 3 days after agroinjection with the 35S- NbAELP:3xFLAG vector. Cell homogenate was fractionated into CW, P1, P30, S30 (fraction of soluble cytoplasmic proteins) (B) and apoplast (C) fractions. The lower panels show the protein loading control stained with Amido Black.
    Dylight 488 Ddk Mab, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    OriGene mouse anti ddk
    NbAELP of agroinjected leaves was revealed mainly in the cell wall fraction. (A) Western blot analysis with NbAELP-specific antibodies of subcellular fractions of N. benthamiana leaves at 3 day after agroinjection with A. tumefaciens containing “empty” vector (pBin19). AEP, porcine kidney mutarotase “Calzyme” (~400 ng). CW, cell wall fraction; P1, fraction enriched with nuclear proteins; P30, fraction containing mainly membrane proteins from Golgi apparatus and endoplasmic reticulum. (B,C) Western blot analysis using <t>anti-FLAG</t> antibodies of N. benthamiana leaf proteins at 3 days after agroinjection with the 35S- NbAELP:3xFLAG vector. Cell homogenate was fractionated into CW, P1, P30, S30 (fraction of soluble cytoplasmic proteins) (B) and apoplast (C) fractions. The lower panels show the protein loading control stained with Amido Black.
    Mouse Anti Ddk, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    NbAELP of agroinjected leaves was revealed mainly in the cell wall fraction. (A) Western blot analysis with NbAELP-specific antibodies of subcellular fractions of N. benthamiana leaves at 3 day after agroinjection with A. tumefaciens containing “empty” vector (pBin19). AEP, porcine kidney mutarotase “Calzyme” (~400 ng). CW, cell wall fraction; P1, fraction enriched with nuclear proteins; P30, fraction containing mainly membrane proteins from Golgi apparatus and endoplasmic reticulum. (B,C) Western blot analysis using anti-FLAG antibodies of N. benthamiana leaf proteins at 3 days after agroinjection with the 35S- NbAELP:3xFLAG vector. Cell homogenate was fractionated into CW, P1, P30, S30 (fraction of soluble cytoplasmic proteins) (B) and apoplast (C) fractions. The lower panels show the protein loading control stained with Amido Black.

    Journal: Frontiers in Plant Science

    Article Title: The Intergenic Interplay between Aldose 1-Epimerase-Like Protein and Pectin Methylesterase in Abiotic and Biotic Stress Control

    doi: 10.3389/fpls.2017.01646

    Figure Lengend Snippet: NbAELP of agroinjected leaves was revealed mainly in the cell wall fraction. (A) Western blot analysis with NbAELP-specific antibodies of subcellular fractions of N. benthamiana leaves at 3 day after agroinjection with A. tumefaciens containing “empty” vector (pBin19). AEP, porcine kidney mutarotase “Calzyme” (~400 ng). CW, cell wall fraction; P1, fraction enriched with nuclear proteins; P30, fraction containing mainly membrane proteins from Golgi apparatus and endoplasmic reticulum. (B,C) Western blot analysis using anti-FLAG antibodies of N. benthamiana leaf proteins at 3 days after agroinjection with the 35S- NbAELP:3xFLAG vector. Cell homogenate was fractionated into CW, P1, P30, S30 (fraction of soluble cytoplasmic proteins) (B) and apoplast (C) fractions. The lower panels show the protein loading control stained with Amido Black.

    Article Snippet: For GFP, NbAELP, FLAG or TMV MP detection, the membranes were probed with corresponding antibodies: goat anti-GFP antibodies conjugated with horseradish peroxidase (Rockland Immunochemicals), mouse anti-FLAG monoclonal antibodies (Sigma), mouse polyclonal antibodies against recombinant NbAELP or TMV MP.

    Techniques: Western Blot, Plasmid Preparation, Staining