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Native Antigen Inc
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Proteintech
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Proteintech
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Santa Cruz Biotechnology
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Proteintech
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Biozol Diagnostica Vertrieb GmbH
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Insight Biotechnology Ltd
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SeraCare Life Sciences
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GeneTex
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INCSTAR Corporation
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srl inc
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Selcia Inc
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Image Search Results
Journal: Investigative Ophthalmology & Visual Science
Article Title: Insufficient Dose of ERCC8 Protein Caused by a Frameshift Mutation Is Associated With Keratoconus With Congenital Cataracts
doi: 10.1167/iovs.63.13.1
Figure Lengend Snippet: Pedigree and genetic mutation of the family with keratoconus and congenital cataracts. ( A ) Pedigree. ( B ) Sanger sequencing confirms the c.394-398del (p. L132Nfs*6) mutation identified by WES (I-2 and II-1) and HRM (KC5). ( C ) Cross-species comparison of the region of ERCC8 indicates that the identified mutation affects highly conserved residues. ( D ) Domain analysis of the p. L132Nfs*6 mutation. ( E ) The 3D structures of mutant and wild type protein.
Article Snippet: Primary antibodies included those of collagen IV (ab6586; Abcam),
Techniques: Mutagenesis, Sequencing, Comparison
Journal: Investigative Ophthalmology & Visual Science
Article Title: Insufficient Dose of ERCC8 Protein Caused by a Frameshift Mutation Is Associated With Keratoconus With Congenital Cataracts
doi: 10.1167/iovs.63.13.1
Figure Lengend Snippet: Pathogenicity Assessment of ERCC8 Mutation
Article Snippet: Primary antibodies included those of collagen IV (ab6586; Abcam),
Techniques: Mutagenesis, Sequencing, Control
Journal: Investigative Ophthalmology & Visual Science
Article Title: Insufficient Dose of ERCC8 Protein Caused by a Frameshift Mutation Is Associated With Keratoconus With Congenital Cataracts
doi: 10.1167/iovs.63.13.1
Figure Lengend Snippet: Expression of ERCC8 in different mouse tissues. ( A ) Representative figures of immunofluorescence staining of ERCC8 in the mouse eye (the upper was a panoramic image, and the lower were local images). ( B ) The mRNA expression levels of ERCC8 in different mouse tissues.
Article Snippet: Primary antibodies included those of collagen IV (ab6586; Abcam),
Techniques: Expressing, Immunofluorescence, Staining
Journal: Investigative Ophthalmology & Visual Science
Article Title: Insufficient Dose of ERCC8 Protein Caused by a Frameshift Mutation Is Associated With Keratoconus With Congenital Cataracts
doi: 10.1167/iovs.63.13.1
Figure Lengend Snippet: Mutant ERCC8 constructs and expression in human embryonic kidney 293 cells. ( A ) The fluorescence intensity of GFP after transfection for 48 hours. ( B ) mRNA expression levels of ERCC8 and TCR-related genes ( CSB , XPA , RPA , and XPG ) in overexpressed wild type and mutant cells. ( C ) Western blot results of ERCC8 protein in overexpressed wild type and mutant cells. ( D ) Statistical results of the Western blot; N = 3, *, P < 0.05.
Article Snippet: Primary antibodies included those of collagen IV (ab6586; Abcam),
Techniques: Mutagenesis, Construct, Expressing, Fluorescence, Transfection, Western Blot
Journal: Investigative Ophthalmology & Visual Science
Article Title: Insufficient Dose of ERCC8 Protein Caused by a Frameshift Mutation Is Associated With Keratoconus With Congenital Cataracts
doi: 10.1167/iovs.63.13.1
Figure Lengend Snippet: Knockdown of ERCC8 in human corneal fibroblast cells. ( A ) The mRNA expression levels of ERCC8 after being transfected by ERCC8 siRNA. ( B ) The mRNA expression levels of TCR-related genes ( CSB , XPA , RPA , and XPG ) after being transfected by ERCC8 siRNA. ( C ) Western blot results of ERCC8, collagen Ⅳ, and SOD1 proteins after being transfected by ERCC8 siRNA. ( D ) Statistical results of the Western blot; N = 3, *, P < 0.05.
Article Snippet: Primary antibodies included those of collagen IV (ab6586; Abcam),
Techniques: Knockdown, Expressing, Transfection, Western Blot
Journal: Investigative Ophthalmology & Visual Science
Article Title: Insufficient Dose of ERCC8 Protein Caused by a Frameshift Mutation Is Associated With Keratoconus With Congenital Cataracts
doi: 10.1167/iovs.63.13.1
Figure Lengend Snippet: Cell viability and DNA damage assays of HTK treated by H 2 O 2 after ERCC8 siRNA transfection. ( A ) Cell viability assay results. ( B ) Hoechst staining results. ( C ) Western blot results of ERCC8, γ- H2AX, and phospho p65 protein. ( D ) Statistical results of the Western blot; N = 3, *, P < 0.05.
Article Snippet: Primary antibodies included those of collagen IV (ab6586; Abcam),
Techniques: Transfection, Viability Assay, Staining, Western Blot
Journal: Investigative Ophthalmology & Visual Science
Article Title: Insufficient Dose of ERCC8 Protein Caused by a Frameshift Mutation Is Associated With Keratoconus With Congenital Cataracts
doi: 10.1167/iovs.63.13.1
Figure Lengend Snippet: Cell viability, apoptosis, and DNA damage assays of HLECs treated by H 2 O 2 after ERCC8 siRNA transfection. ( A ) Cell viability assay results. ( B ) The mRNA expression levels of TCR-related genes. ( C ) Representative figures of immunofluorescence staining of cell apoptosis. ( D ) Statistical results of the cell apoptosis analysis. ( E ) The mRNA expression levels of UPR-related genes. ( F ) Western blot results of SOD1, γ- H2AX, and caspase 3 protein. ( D ) Statistical results of the Western blot; N = 3, *, P < 0.05.
Article Snippet: Primary antibodies included those of collagen IV (ab6586; Abcam),
Techniques: Transfection, Viability Assay, Expressing, Immunofluorescence, Staining, Western Blot