Journal: Toxins

Article Title: PTX Instructs the Development of Lung-Resident Memory T Cells in Bordetella pertussis Infected Mice

doi: 10.3390/toxins13090632

Figure Lengend Snippet: The generation of Trm cells is dependent on PTX exposure during B. pertussis infection. ( A ) Representative flow cytometry of CD69 and CD103 expression of CD62L − CD44 + CD4 + T cells harvested from lung in ΔPTX, pPTX, ΔPTX + pPTX and WT groups at day 15 p.i. Frequency and absolute numbers of ( B ) CD4 + Trm and ( C ) CD8 + Trm harvested in the lung of ΔPTX, pPTX, ΔPTX+pPTX and WT groups at day 15 p.i. Results are mean ± SD for at least 3 to 5 mice/group and are representative of two independent experiments. Statistical significance of differences are shown. * p -value < 0.05; ** p -value < 0.01, *** p -value < 0.001 and **** p -value < 0.0001.

Article Snippet: T cells were enriched negatively by using Pan T kit supplemented with CD62L antibody (MiltenyiBiotec, Paris, France).

Techniques: Infection, Flow Cytometry, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Platelet Extracellular Vesicles Are Taken up by Canine T Lymphocytes but Do Not Play a Role in Their Proliferation, Differentiation and Cytokine Production In Vitro

doi: 10.3390/ijms23105504

Figure Lengend Snippet: List of monoclonal antibodies used for labeling peripheral blood mononuclear cells (PBMCs) for flow cytometry. Abbreviations: FITC—Fluorescein isothiocyanate, PE—Phycoerythrin, AF647—Alexa Fluor 647, PB—Pacific Blue.

Article Snippet: CD62L , FMC46 , IgG2b , mouse , PB , MCA1076PB , BioRad , 1:5.

Techniques: Bioprocessing, Labeling, Flow Cytometry

Journal: Nature Communications

Article Title: Centrioles are frequently amplified in early B cell development but dispensable for humoral immunity

doi: 10.1038/s41467-024-53222-4

Figure Lengend Snippet:

Article Snippet: , αCD62L , Miltenyi Biotec , 130-112-836 , REA828 , 1:200.

Techniques:

Journal: Cancer Cell

Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity

doi: 10.1016/j.ccell.2021.06.017

Figure Lengend Snippet:

Article Snippet: Anti-mouse CD62L 160Gd clone MEL-14 , Fluidigm , 3160008B.

Techniques: Blocking Assay, Virus, Recombinant, Plasmid Preparation, Saline, Lysis, Staining, Reverse Transcription, In Vivo, Electroporation, Mass Cytometry, Conjugation Assay, Illumina Sequencing, Library Quantification, In Vitro, Quantitative RT-PCR, Software, Real-time Polymerase Chain Reaction, Cytometry, Microscopy, Imaging, Spectrophotometry

Journal: PLoS ONE

Article Title: T Cell and Dendritic Cell Abnormalities Synergize to Expand Pro-Inflammatory T Cell Subsets Leading to Fatal Autoimmunity in B6.NZBc1 Lupus-Prone Mice

doi: 10.1371/journal.pone.0075166

Figure Lengend Snippet: (A) Splenocytes from 4-mo-old mice were stained to assess the proportion of Tfh (CD4 + CD44 hi CD62L lo CXCR5 hi PD1 hi ) cells. Representative contour plots from B6 and c1(70-100) mice. Thick boxes denote the regions that were used to identify Tfh cells. Cells shown in the right panels were gated on the regions shown in the left panels. (B) Scatter plots showing the proportion of Tfh cells within the CD4 + T cell subset and absolute number of splenic Tfh cells. (C) Representative contour plots and histograms from flow cytometry analysis of IL-17-, IFN-γ-, and IL-4-expressing CD4 + T cells in B6 and c1(70-100) mice. Splenocytes were stimulated with PMA and ionomycin in the presence of GolgiStop for 4 h, and then fixed, stained with anti-CD3 and -CD4, permeabilized, and stained with anti-cytokine Ab. Thick lines outline the regions used to gate CD4 + CD3 + T cells. For histograms, the percentage of cells staining positively for each cytokine is indicated. (D) Scatterplots showing the percentages of cytokine-producing cells as a proportion of the CD4 + T cell population. Horizontal lines indicate the mean of each group examined. Significance levels were determined by one-way ANOVA with Dunns’ post-test. The p values for significant differences between B6 and congenic mouse strains are shown with **p<0.01, ***p<0.001. Bars with p values above denote significant differences between congenic strains.

Article Snippet: Biotin-conjugated peanut agglutinin (PNA) was purchased from Sigma-Aldrich and FITC-conjugated anti-CD62L (MEL-14) mAb was purchased from Cedarlane Laboratories (Burlington, ON, Canada).

Techniques: Staining, Flow Cytometry, Expressing

Journal: PLoS ONE

Article Title: T Cell and Dendritic Cell Abnormalities Synergize to Expand Pro-Inflammatory T Cell Subsets Leading to Fatal Autoimmunity in B6.NZBc1 Lupus-Prone Mice

doi: 10.1371/journal.pone.0075166

Figure Lengend Snippet: 8-wk-old mice were injected i.p. with OVA or PBS in CFA. The proportions of splenic Tfh cells (CD4 + CD44 hi CD62L lo CXCR5 hi PD1 hi ) and splenic GC B cells (B220 + Fas + PNA hi ) were determined by flow cytometry 2 wks later. (A) Scatterplots showing the proportion of Tfh and GC B cells as a proportion of the CD4 + T cell and B220 + B cell populations, respectively. (B) Scatterplots showing the amount of cytokine produced by OVA-primed splenocytes re-stimulated in-vitro with OVA for 72h. Assays were performed in triplicate and the levels of secreted cytokines measured by ELISA or cytokine bead array (see Methods). Each data point represents the mean of the triplicate with background cytokine production in the absence of antigen subtracted. Horizontal lines indicate the mean of each group examined. Significance levels were determined by one-way ANOVA with Dunns’ post-test. The p values for significant differences between B6 and congenic mouse strains are shown with *p<0.05, **p<0.01, ***p<0.001.

Article Snippet: Biotin-conjugated peanut agglutinin (PNA) was purchased from Sigma-Aldrich and FITC-conjugated anti-CD62L (MEL-14) mAb was purchased from Cedarlane Laboratories (Burlington, ON, Canada).

Techniques: Injection, Flow Cytometry, Produced, In Vitro, Enzyme-linked Immunosorbent Assay

Journal: Cell regeneration (London, England)

Article Title: Human induced pluripotent stem cells derived neutrophils display strong anti-microbial potencies.

doi: 10.1186/s13619-025-00227-z

Figure Lengend Snippet: Fig. 1 Generation of neutrophils from hiPSCs. A Representative brightfield images of different stages of iNEUs differentiation from hiPSCs. Scale bar, 50 μm. B, C Representative FACS data of indicated markers on iHSPCs (B) and iNEUs differentiation at different time points (C). D Wright–Giemsa images of iNEUs and PB-NEUs. Scale bar, 10 μm. E Representative FACS data of indicated surface markers (CD15, CD66b, CD11b, CD18, CD62L and CD16; red filled) on iNEUs and PB-NEUs (blue, respective isotype control). F Estimated number of iHSPCs and iNEUs obtained from 3 × 106 hiPSCs cultured in 1 T150 flask

Article Snippet: The following antibodies were used to analyze cell surface markers: APC-conjugated anti-human CD11b (301310; Biolegend), APC-conjugated anti-human APC-conjugated anti-human CD15 (561716; BD Pharmingen), PEconjugated anti-human CD16 (555407; BD Biosciences), PE-conjugated anti-human CD62L (E-AB-F1051D, Elabscience), FITC-conjugated anti-human CD18 (101405; Biolegend), Percpcy5.5-conjugated anti-human CD66b (396914; BD Pharmingen).

Techniques: Control, Cell Culture

Journal: STAR Protocols

Article Title: CyTOF protocol for immune monitoring of solid tumors from mouse models

doi: 10.1016/j.xpro.2022.101949

Figure Lengend Snippet:

Article Snippet: Anti-Mouse CD62L-164Dy dilution 1:100 , Standard BioTools , 3164003B.

Techniques: Blocking Assay, Purification, Recombinant, Staining, Sterility, Antibody Labeling, Software, Transferring, Microscopy