Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Geniposide ameliorates glucocorticoid-induced osteoblast apoptosis by activating autophagy.

doi: 10.1016/j.biopha.2022.113829

Figure Lengend Snippet: Fig. 4. GEN inhibited DEX-induced apoptosis by activating autophagy. The immunohistochemical changes (× 40 magnification) of cleaved caspase3 (A, B) were analyzed. The proteins expression of P62 (C, D), LC3-II/I (C, E), Beclin-1 (C, F), and cleaved caspase3 (C, G) in DEX-treated MC3T3-E1 cells were determined by western blot. (H, I) The apoptosis was determined by flow cytometer. **P˂0.01. NC, negative control group; DEX, model rat group treated with DEX (5 mg/kg).

Article Snippet: AF0016), m-TOR (1:1000 dilution; Affinity, cat.no.AF6308), P-m-TOR J. Huang et al. Biomedicine & Pharmacotherapy 155 (2022) 113829 (1:1000 dilution; Affinity, cat.no.AF3308), cleaved-caspase3 (1:1000 dilution; Affinity, cat.no.AF7022), and β-actin (1:1000 dilution; Affinity, cat.no.AF7018), and then with HRP-labeled goat anti-rabbit secondary antibody (1:5000 dilution; Boster Biological Technology, Wuhan, China, cat.no.BA1039).

Techniques: Immunohistochemical staining, Expressing, Western Blot, Flow Cytometry, Negative Control

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Geniposide ameliorates glucocorticoid-induced osteoblast apoptosis by activating autophagy.

doi: 10.1016/j.biopha.2022.113829

Figure Lengend Snippet: Fig. 5. GEN activated autophagy by inhibiting PI3K activity. The proteins expression of PI3K (A, B), P-PI3K (A, C), AKT (A, D), P-AKT (A, E), mTOR (A, F), P-mTOR (A, G), P62 (A, H), LC3-II/I (A, I), Beclin-1 (A, J), and cleaved caspase3 (A, K) in DEX-treated MC3T3-E1 cells were determined by western blot. (L, M) The apoptosis was determined by a flow cytometer. **P˂0.01.

Article Snippet: AF0016), m-TOR (1:1000 dilution; Affinity, cat.no.AF6308), P-m-TOR J. Huang et al. Biomedicine & Pharmacotherapy 155 (2022) 113829 (1:1000 dilution; Affinity, cat.no.AF3308), cleaved-caspase3 (1:1000 dilution; Affinity, cat.no.AF7022), and β-actin (1:1000 dilution; Affinity, cat.no.AF7018), and then with HRP-labeled goat anti-rabbit secondary antibody (1:5000 dilution; Boster Biological Technology, Wuhan, China, cat.no.BA1039).

Techniques: Activity Assay, Expressing, Western Blot, Flow Cytometry

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Geniposide ameliorates glucocorticoid-induced osteoblast apoptosis by activating autophagy.

doi: 10.1016/j.biopha.2022.113829

Figure Lengend Snippet: Fig. 6. GEN activated autophagy by inhibiting AKT activity. The proteins expression of AKT (A, B), P-AKT (A, C), mTOR (A, D), P-mTOR (A, E), P62 (A, F), LC3-II/I (A, G), Beclin-1 (A, H), and cleaved caspase3 (A, I) in DEX-treated MC3T3-E1 cells were determined by western blot. (J, K) The apoptosis was determined by a flow cytometer. **P˂0.01.

Article Snippet: AF0016), m-TOR (1:1000 dilution; Affinity, cat.no.AF6308), P-m-TOR J. Huang et al. Biomedicine & Pharmacotherapy 155 (2022) 113829 (1:1000 dilution; Affinity, cat.no.AF3308), cleaved-caspase3 (1:1000 dilution; Affinity, cat.no.AF7022), and β-actin (1:1000 dilution; Affinity, cat.no.AF7018), and then with HRP-labeled goat anti-rabbit secondary antibody (1:5000 dilution; Boster Biological Technology, Wuhan, China, cat.no.BA1039).

Techniques: Activity Assay, Expressing, Western Blot, Flow Cytometry

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Geniposide ameliorates glucocorticoid-induced osteoblast apoptosis by activating autophagy.

doi: 10.1016/j.biopha.2022.113829

Figure Lengend Snippet: Fig. 7. GEN activated autophagy by inhibiting mTOR activity. The proteins expression of mTOR (A, B), P-mTOR (A, C), P62 (A, D), LC3-II/I (A, E), Beclin-1 (A, F), and cleaved caspase3 (A, G) in DEX-treated MC3T3-E1 cells were determined by western blot. (H, I) The apoptosis was determined by a flow cytometer. * *P˂0.01.

Article Snippet: AF0016), m-TOR (1:1000 dilution; Affinity, cat.no.AF6308), P-m-TOR J. Huang et al. Biomedicine & Pharmacotherapy 155 (2022) 113829 (1:1000 dilution; Affinity, cat.no.AF3308), cleaved-caspase3 (1:1000 dilution; Affinity, cat.no.AF7022), and β-actin (1:1000 dilution; Affinity, cat.no.AF7018), and then with HRP-labeled goat anti-rabbit secondary antibody (1:5000 dilution; Boster Biological Technology, Wuhan, China, cat.no.BA1039).

Techniques: Activity Assay, Expressing, Western Blot, Flow Cytometry

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Geniposide ameliorates glucocorticoid-induced osteoblast apoptosis by activating autophagy.

doi: 10.1016/j.biopha.2022.113829

Figure Lengend Snippet: Fig. 8. GEN mediated autophagy by stimulating GLP-1R activity. The proteins expression of GLP-1R (A, B), PI3K (A, C), P-PI3K (A, D), AKT (A, E), P-AKT (A, F), mTOR (A, G), P-mTOR (A, H), P62 (A, I), LC3-II/I (A, J), Beclin-1 (A, K), and cleaved caspase3 (A, L) in DEX-treated MC3T3-E1 cells were determined by western blot. (M, N) The apoptosis was determined by a flow cytometer. **P˂0.01.

Article Snippet: AF0016), m-TOR (1:1000 dilution; Affinity, cat.no.AF6308), P-m-TOR J. Huang et al. Biomedicine & Pharmacotherapy 155 (2022) 113829 (1:1000 dilution; Affinity, cat.no.AF3308), cleaved-caspase3 (1:1000 dilution; Affinity, cat.no.AF7022), and β-actin (1:1000 dilution; Affinity, cat.no.AF7018), and then with HRP-labeled goat anti-rabbit secondary antibody (1:5000 dilution; Boster Biological Technology, Wuhan, China, cat.no.BA1039).

Techniques: Activity Assay, Expressing, Western Blot, Flow Cytometry