Journal: Hypertension

Article Title: Identification of Bona Fide Alternative Renin Transcripts Expressed Along Cortical Tubules and Potential Roles in Promoting Insulin Resistance In Vivo Without Significant Plasma Renin Activity Elevation

doi: 10.1161/hypertensionaha.114.03394

Figure Lengend Snippet: Figure 7. A, The results of quantitative reverse transcription polymerase chain reaction analyses of inflammatory molecules in heart, kidney, and liver of both ARen2TG and wild-type littermates are shown as relative abundance, using GAPDH expression as an internal control. *, **, #, ##Significantly elevated compared with the expressions of wild-type littermates. Repeated measure ANOVA and subsequent post hoc analyses were performed. B, Representative results of in situ histological examinations for livers of both transgenic mice and wild-type littermate using rabbit polyclonal anti-angiotensin II antibodies (NBP1-30027; Novus Biologicals, LLC, Littleton, CO) were shown. a–c, Enhanced immunohistochemical staining in livers of transgenic mice compared with those of wild-type littermates (Bd), suggesting enhanced angiotensin II production in the organs.

Article Snippet: We performed experiments to compare ARen2TG mice with wild-type littermates in terms of following phenotypes: measurement of blood pressure,16,17 measurement of plasma renin activity,16,18,19 the results of in vivo near-infrared imaging using the renin substrate analog ReninSense 680 FAST (Perkin Elmer, Waltham, MA),20 the results of insulin tolerance tests,21 fasting plasma glucose levels, the expressions of inflammatory molecules in various tissues on quantitative RT-PCR analyses,16,18,19 and in situ histological examinations for livers of both transgenic mice and wild-type littermate using rabbit polyclonal anti-angiotensin II antibodies (NBP1-30027; Novus Biologicals, LLC, Littleton, CO).22,23 All experiments were performed according to the protocols of manufacturer, the references, and our reports as specified above.

Techniques: Reverse Transcription, Polymerase Chain Reaction, Expressing, Control, In Situ, Transgenic Assay, Immunohistochemical staining, Staining

Journal: Acta Biochimica et Biophysica Sinica

Article Title: Activation of angiogenin expression in macrophages by lipopolysaccharide via the TLR4/NF-κB pathway in colitis

doi: 10.3724/abbs.2024013

Figure Lengend Snippet: Table 1 Sequences of primers and siRNAs used in this study

Article Snippet: The membrane was blocked with 5% nonfat milk and then incubated with primary antibodies, including anti-ANG antibody (prepared in our own laboratory) and anti-ACTB antibody (#81115-1-RR; Proteintech, Chicago, USA), in TBST (Tris-buffered saline, 0.1% Tween 20) buffer at 4°C overnight.

Techniques: Negative Control

Journal: Risk Management and Healthcare Policy

Article Title: Effects of AGT and AGTR1 Genetic Polymorphisms and Changes in Blood Pressure Over a Five-Year Follow-Up

doi: 10.2147/RMHP.S442983

Figure Lengend Snippet: The Hardy–Weinberg Equilibrium for AGT rs699 and AGTR1 rs5186 in the Study Population (P <0.05 Indicates Results are Not Consistent with the Hardy–Weinberg Equilibrium)

Article Snippet: TaqMan SNP genotyping primers and probes were designed by Applied Biosystems (rs699 assay ID: C_1985481_20 and rs5186 assay ID: C_3187716_10).

Techniques:

Journal: Risk Management and Healthcare Policy

Article Title: Effects of AGT and AGTR1 Genetic Polymorphisms and Changes in Blood Pressure Over a Five-Year Follow-Up

doi: 10.2147/RMHP.S442983

Figure Lengend Snippet: Baseline Characteristics of the Study Cohort Classified by SNPs and Genotype

Article Snippet: TaqMan SNP genotyping primers and probes were designed by Applied Biosystems (rs699 assay ID: C_1985481_20 and rs5186 assay ID: C_3187716_10).

Techniques:

Journal: Risk Management and Healthcare Policy

Article Title: Effects of AGT and AGTR1 Genetic Polymorphisms and Changes in Blood Pressure Over a Five-Year Follow-Up

doi: 10.2147/RMHP.S442983

Figure Lengend Snippet: Associations Between AGT and AGTR1 Polymorphisms and Changes in SBP and DBP at Baseline and at the 5-Year Follow-Up According to Mode of Inheritance

Article Snippet: TaqMan SNP genotyping primers and probes were designed by Applied Biosystems (rs699 assay ID: C_1985481_20 and rs5186 assay ID: C_3187716_10).

Techniques:

Journal: Risk Management and Healthcare Policy

Article Title: Effects of AGT and AGTR1 Genetic Polymorphisms and Changes in Blood Pressure Over a Five-Year Follow-Up

doi: 10.2147/RMHP.S442983

Figure Lengend Snippet: Association Between Haplotype Variations in AGT rs699 and AGTR1 rs5186 and SBP and DBP at Baseline and at the 5-Year Follow-Up

Article Snippet: TaqMan SNP genotyping primers and probes were designed by Applied Biosystems (rs699 assay ID: C_1985481_20 and rs5186 assay ID: C_3187716_10).

Techniques:

Journal: Risk Management and Healthcare Policy

Article Title: Effects of AGT and AGTR1 Genetic Polymorphisms and Changes in Blood Pressure Over a Five-Year Follow-Up

doi: 10.2147/RMHP.S442983

Figure Lengend Snippet: Odds ratios of hypertension and hypertension with metabolic syndrome depending on haplotype variations of AGT rs699 and AGTR1 rs5186.

Article Snippet: TaqMan SNP genotyping primers and probes were designed by Applied Biosystems (rs699 assay ID: C_1985481_20 and rs5186 assay ID: C_3187716_10).

Techniques:

Journal: Risk Management and Healthcare Policy

Article Title: Effects of AGT and AGTR1 Genetic Polymorphisms and Changes in Blood Pressure Over a Five-Year Follow-Up

doi: 10.2147/RMHP.S442983

Figure Lengend Snippet: A network of AGT and AGTR1 gene interactions using GeneMania, ( http://genemania.org ).

Article Snippet: TaqMan SNP genotyping primers and probes were designed by Applied Biosystems (rs699 assay ID: C_1985481_20 and rs5186 assay ID: C_3187716_10).

Techniques:

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Collecting Duct Nitric Oxide Synthase 1ß Activation Maintains Sodium Homeostasis During High Sodium Intake Through Suppression of Aldosterone and Renal Angiotensin II Pathways

doi: 10.1161/JAHA.117.006896

Figure Lengend Snippet: Antibodies Used in This Study

Article Snippet: After a 20‐minute protein block step (Biocare, Rodent Block M), slides were incubated in 1 μg/mL anti‐mouse/rat angiotensinogen (rabbit IgG affinity purified, IBL America) overnight in a humidified chamber at 4°C.

Techniques: Concentration Assay

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Collecting Duct Nitric Oxide Synthase 1ß Activation Maintains Sodium Homeostasis During High Sodium Intake Through Suppression of Aldosterone and Renal Angiotensin II Pathways

doi: 10.1161/JAHA.117.006896

Figure Lengend Snippet: Protein expression of angiotensinogen (Agt) and angiotensin II (Ang II ) in flox control and collecting duct nitric oxide synthase 1β knockout (CDNOS1 KO ) mice and cortical mRNA expression of the Ang II axis. Mice were fed a low sodium ( LS ), normal sodium ( NS ), or high sodium ( HS ) diet for 1 week. A, Representative sections of cortex stained with anti‐Ang II or anti‐Agt. Negative controls lack primary antibodies. B, Cortical Ang II content from control and CDNOS 1 KO mice on an HS diet (n=5/group). C through I, Relative to control NS , cortical mRNA expression of (C) Agt , (D) renin , (E) angiotensin‐converting enzyme ( ACE ) 1 , (F) ACE 2 , (G) angiotensin II receptor 1a ( AT 1a ), (H) AT 1b , and (I) AT 2 (n=4/group). *Represents P <0.05 compared with control, † P <0.05 compared with NS. KO indicates knockout.

Article Snippet: After a 20‐minute protein block step (Biocare, Rodent Block M), slides were incubated in 1 μg/mL anti‐mouse/rat angiotensinogen (rabbit IgG affinity purified, IBL America) overnight in a humidified chamber at 4°C.

Techniques: Expressing, Control, Knock-Out, Staining

Journal: Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease

Article Title: Collecting Duct Nitric Oxide Synthase 1ß Activation Maintains Sodium Homeostasis During High Sodium Intake Through Suppression of Aldosterone and Renal Angiotensin II Pathways

doi: 10.1161/JAHA.117.006896

Figure Lengend Snippet: Protein abundance of angiotensinogen (Agt), renin, and angiotensin‐converting enzyme ( ACE ). A, Representative Western blots of cortical prorenin/renin, Agt, and loading control actin‐ and Coomassie Blue–stained membranes. Numbers are relative to control normal sodium ( NS ) densitometry and are mean±SEM NS (n=5/group) and high sodium (HS; n=3/group). B, Representative Western blots of prorenin expression in the inner medulla of mice on an HS diet (n=6/group). C, Representative Western blots of cortical ACE 1 and ACE 2. Numbers are relative to control densitometry and are mean±SEM HS (n=3/group). D, Cortical ACE activity after 1 week of an HS diet as expressed relative to control ACE activity. Control mice (N=7) and collecting duct nitric oxide synthase 1β knockout (CDNOS1KO) mice (N=4). KO indicates knockout.

Article Snippet: After a 20‐minute protein block step (Biocare, Rodent Block M), slides were incubated in 1 μg/mL anti‐mouse/rat angiotensinogen (rabbit IgG affinity purified, IBL America) overnight in a humidified chamber at 4°C.

Techniques: Quantitative Proteomics, Western Blot, Control, Staining, Expressing, Activity Assay, Knock-Out