Journal: Evidence-based complementary and alternative medicine : eCAM

Article Title: Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma.

doi: 10.1155/2021/5526581

Figure Lengend Snippet: Figure 3: Effects of andrographolide on the migration ability of 8505C and CAL 62 cells. (a) .e effect of andrographolide on the migration ability of 8505C cells. (b) .e effect of andrographolide on the migration ability of CAL62 cells.

Article Snippet: Andrographolide (TargetMol), RPMI Medium Modified (HyClone), fetal bovine serum (Gibco), PBS (Gibco), pancreatin (SEGMA), PVDF membrane (Shanghai Beyotime Biotechnology Co., Ltd.), SDS-PAGE gel rapid preparation kit (Shanghai Beyotime Biotechnology Co., Ltd.), milk powder (Biofroxx), 20xTBST (Solarbio), CCK8 (ApexBio), Annexin V-EGFP Apoptosis Detection Kit (Hangzhou Lianke Biotechnology Co., Ltd.), cycle detection kit (Hangzhou LinkTech Biotechnology Co., Ltd.), BCA protein concentration determination kit (Shanghai Beyotime Biotechnology Co., Ltd.), RIPA Lysis Buffer (Shanghai Beyotime Biotechnology Co., Ltd.), PMSF (Shanghai Beyotime Biotechnology Co., Ltd.), a lactic acid detection kit (Nanjing Jiancheng Biological Engineering Institute Co., Ltd.), and a glucose detection kit (Shanghai Rongsheng Biopharmaceutical Industry Co., Ltd.) were used in this research, and all of the antibodies were purchased from Proteintech.

Techniques: Migration

Journal: Evidence-based complementary and alternative medicine : eCAM

Article Title: Study on the Antitumor Effect and Glycolysis of Andrographolide in Anaplastic Thyroid Carcinoma.

doi: 10.1155/2021/5526581

Figure Lengend Snippet: Figure 4: Effect of andrographolide on the cell cycle in 8505C and CAL62 ATC cells. (a) Cell cycle distribution of 8505C cells. (b) Cell cycle distribution of 8505C cells after andrographolide treatment. (c) Cell cycle distribution statistics of 8505C cells after treatment of 8505C cells with andrographolide. (d) Cell cycle distribution of CAL62 cells. (e) Cell cycle distribution of CAL62 cells after andrographolide treatment. (f) Cell cycle distribution statistics of CAL62 cells after the actions of CAL62 and andrographolide.

Article Snippet: Andrographolide (TargetMol), RPMI Medium Modified (HyClone), fetal bovine serum (Gibco), PBS (Gibco), pancreatin (SEGMA), PVDF membrane (Shanghai Beyotime Biotechnology Co., Ltd.), SDS-PAGE gel rapid preparation kit (Shanghai Beyotime Biotechnology Co., Ltd.), milk powder (Biofroxx), 20xTBST (Solarbio), CCK8 (ApexBio), Annexin V-EGFP Apoptosis Detection Kit (Hangzhou Lianke Biotechnology Co., Ltd.), cycle detection kit (Hangzhou LinkTech Biotechnology Co., Ltd.), BCA protein concentration determination kit (Shanghai Beyotime Biotechnology Co., Ltd.), RIPA Lysis Buffer (Shanghai Beyotime Biotechnology Co., Ltd.), PMSF (Shanghai Beyotime Biotechnology Co., Ltd.), a lactic acid detection kit (Nanjing Jiancheng Biological Engineering Institute Co., Ltd.), and a glucose detection kit (Shanghai Rongsheng Biopharmaceutical Industry Co., Ltd.) were used in this research, and all of the antibodies were purchased from Proteintech.

Techniques:

Journal: Oxidative Medicine and Cellular Longevity

Article Title: Endothelial Cell-Derived SO 2 Controls Endothelial Cell Inflammation, Smooth Muscle Cell Proliferation, and Collagen Synthesis to Inhibit Hypoxic Pulmonary Vascular Remodelling

doi: 10.1155/2021/5577634

Figure Lengend Snippet: EC-derived SO 2 inhibits p50 activation to repress PAEC inflammation, PASMC proliferation, and collagen deposition. (a) Immunofluorescence in situ detection of p50 protein distribution in HPAECs. Red fluorescence represents p50 protein, and DAPI staining labels HPAEC nuclei. (b) Immunofluorescence in situ detection of p50 protein distribution in HPASMCs cocultured with HPAECs. Green fluorescence represents p50 protein, and DAPI staining labels HPASMC nuclei. (c) Western blot analysis was used to detect the expression level of ICAM-1 and MCP-1 protein in HPAECs ( n = 12). (d, e) Immunofluorescence in situ detection of Ki-67 and collagen I protein expression in HPASMCs cocultured with HPAECs. Green fluorescence represents Ki-67 and collagen I protein. Andro: andrographolide, an inhibitor of p50. Data were expressed as mean ± SEM, ∗ p < 0.05, scale: 20 μ m.

Article Snippet: An inhibitor of p50 andrographolide (4 μ M, Andro, Selleck, USA) was used for experiments [ ].

Techniques: Derivative Assay, Activation Assay, Immunofluorescence, In Situ, Fluorescence, Staining, Western Blot, Expressing

Journal: Data in Brief

Article Title: Synacinn™: Bacterial reverse mutation test data in five histidine-requiring strains of Salmonella Typhimurium

doi: 10.1016/j.dib.2021.107075

Figure Lengend Snippet: Identification (Retention Time) of markers in Synacinn™ formulation.

Article Snippet: Andrographolide (sc-205594A), catechin (sc-204673A), curcumin (sc-200509A) and gallic acid (sc-205704A) were purchased from Santa Cruz Biotechnology, while rosmarinic acid (sc-202796A) was purchased from Chengdu Biopurify Phyto Chemicals Ltd. All test items were stored at room temperature protected from light.

Techniques: Formulation

Journal: Frontiers in Pharmacology

Article Title: Extracts of Andrographis paniculata (Burm.f.) Nees Leaves Exert Anti-Gout Effects by Lowering Uric Acid Levels and Reducing Monosodium Urate Crystal-Induced Inflammation

doi: 10.3389/fphar.2021.787125

Figure Lengend Snippet: Representative HPLC chromatograms of diterpene lactones (i.e., andrographolide, 14-deoxy-11,12-didehydroandrographolide, and neoandrographolide) (A) , ethanol (80%) extract (B) and water extract (C) of Andrographis paniculata leaves.

Article Snippet: Andrographolide (AP, 99.7%), 14-deoxy-11,12-didehydroandrographolide (DAP, < 99.6%) and neoandrographolide (NAP, ≥ 95%) were HPLC grade and acquired from Chromadex (California, United States).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Extracts of Andrographis paniculata (Burm.f.) Nees Leaves Exert Anti-Gout Effects by Lowering Uric Acid Levels and Reducing Monosodium Urate Crystal-Induced Inflammation

doi: 10.3389/fphar.2021.787125

Figure Lengend Snippet: Amount of andrographolide (AP), 14-deoxy-11,12-didehydroandrographolide (DAP), and neoandrographolide (NAP) in 80% of ethanol (EtOH80) and water extracts of Andrographis paniculata leaves analyzed by HPLC.

Article Snippet: Andrographolide (AP, 99.7%), 14-deoxy-11,12-didehydroandrographolide (DAP, < 99.6%) and neoandrographolide (NAP, ≥ 95%) were HPLC grade and acquired from Chromadex (California, United States).

Techniques: Concentration Assay

Journal: Frontiers in Pharmacology

Article Title: Extracts of Andrographis paniculata (Burm.f.) Nees Leaves Exert Anti-Gout Effects by Lowering Uric Acid Levels and Reducing Monosodium Urate Crystal-Induced Inflammation

doi: 10.3389/fphar.2021.787125

Figure Lengend Snippet: Effect of ethanol (80%) extract of Andrographis paniculata leaves, andrographolide and allopurinol on serum uric acid levels in hyperuricemic-induced rats. Data are presented as mean ± SEM ( n = 6). Data were analyzed using one-way ANOVA followed by post hoc Tukey. a Significantly different compared to normal group ( p < 0.05). b Not significantly different compared to normal group ( p > 0.05). c Significantly different compared to hyperuricemic group ( p < 0.05). d Not significantly different compared to allopurinol group ( p > 0.05).

Article Snippet: Andrographolide (AP, 99.7%), 14-deoxy-11,12-didehydroandrographolide (DAP, < 99.6%) and neoandrographolide (NAP, ≥ 95%) were HPLC grade and acquired from Chromadex (California, United States).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Extracts of Andrographis paniculata (Burm.f.) Nees Leaves Exert Anti-Gout Effects by Lowering Uric Acid Levels and Reducing Monosodium Urate Crystal-Induced Inflammation

doi: 10.3389/fphar.2021.787125

Figure Lengend Snippet: Effect of ethanol (80%) extract of Andrographis paniculata leaves, andrographolide and allopurinol on xanthine oxidase activity in rat’s liver.

Article Snippet: Andrographolide (AP, 99.7%), 14-deoxy-11,12-didehydroandrographolide (DAP, < 99.6%) and neoandrographolide (NAP, ≥ 95%) were HPLC grade and acquired from Chromadex (California, United States).

Techniques: Activity Assay, Inhibition

Journal: Frontiers in Pharmacology

Article Title: Extracts of Andrographis paniculata (Burm.f.) Nees Leaves Exert Anti-Gout Effects by Lowering Uric Acid Levels and Reducing Monosodium Urate Crystal-Induced Inflammation

doi: 10.3389/fphar.2021.787125

Figure Lengend Snippet: Effect of ethanol (80%) extract of A. paniculata leaves, andrographolide and allopurinol on protein expressions of renal URAT1 (A) , GLUT9 (B) and OAT1 (C) in hyperuricemic-induced rats obtained from Western blot analysis (D) . Data are presented as mean ± SEM ( n = 6). Data were analyzed using one-way ANOVA followed by post hoc Tukey. a Significantly different compared to normal group ( p < 0.05). b Significantly different compared to hyperuricemic group ( p < 0.05).

Article Snippet: Andrographolide (AP, 99.7%), 14-deoxy-11,12-didehydroandrographolide (DAP, < 99.6%) and neoandrographolide (NAP, ≥ 95%) were HPLC grade and acquired from Chromadex (California, United States).

Techniques: Western Blot

Journal: Frontiers in Pharmacology

Article Title: Extracts of Andrographis paniculata (Burm.f.) Nees Leaves Exert Anti-Gout Effects by Lowering Uric Acid Levels and Reducing Monosodium Urate Crystal-Induced Inflammation

doi: 10.3389/fphar.2021.787125

Figure Lengend Snippet: Viability of human fibroblast-like synoviocyte (HFLS) cells after 27 h of exposure to 80% ethanol (EAP) and water (HAP) extracts of Andrographis paniculata leaves, andrographolide (AP), 14-deoxy-11,12-didehydroandrographolide (DAP), neoandrographolide (NAP), dexamethasome (DEXA), and 0.5% DMSO. Data are presented as mean ± SEM ( n = 3).

Article Snippet: Andrographolide (AP, 99.7%), 14-deoxy-11,12-didehydroandrographolide (DAP, < 99.6%) and neoandrographolide (NAP, ≥ 95%) were HPLC grade and acquired from Chromadex (California, United States).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Extracts of Andrographis paniculata (Burm.f.) Nees Leaves Exert Anti-Gout Effects by Lowering Uric Acid Levels and Reducing Monosodium Urate Crystal-Induced Inflammation

doi: 10.3389/fphar.2021.787125

Figure Lengend Snippet: Effect of ethanol (80%) extract of Andrographis paniculata leaves, andrographolide, and indomethacin on swelling rate in MSU-induced inflammation in rat’s knee joint.

Article Snippet: Andrographolide (AP, 99.7%), 14-deoxy-11,12-didehydroandrographolide (DAP, < 99.6%) and neoandrographolide (NAP, ≥ 95%) were HPLC grade and acquired from Chromadex (California, United States).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Extracts of Andrographis paniculata (Burm.f.) Nees Leaves Exert Anti-Gout Effects by Lowering Uric Acid Levels and Reducing Monosodium Urate Crystal-Induced Inflammation

doi: 10.3389/fphar.2021.787125

Figure Lengend Snippet: Effect of ethanol (80%) extract of Andrographis paniculata leaves and andrographolide on MSU-induced inflammatory mediator secretion in rat’s knee joint synovial fluid: (A) cytokines and (B) prostaglandin E 2 . Data are presented as mean ± SEM ( n = 6). a Significantly different compared to MSU control group ( p < 0.05). b Not significantly different compared to normal control group ( p > 0.05). c Not significantly different compared to indomethacin group ( p > 0.05).

Article Snippet: Andrographolide (AP, 99.7%), 14-deoxy-11,12-didehydroandrographolide (DAP, < 99.6%) and neoandrographolide (NAP, ≥ 95%) were HPLC grade and acquired from Chromadex (California, United States).

Techniques: Control