analyzer software Search Results


tc20 tm data analyzer software  (Bio-Rad)
99
Bio-Rad tc20 tm data analyzer software
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Tc20 Tm Data Analyzer Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
tc20 tm data analyzer software - by Bioz Stars, 2026-06
99/100 stars
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thermal analysis gas station  (Revvity)
90
Revvity thermal analysis gas station
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Thermal Analysis Gas Station, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
thermal analysis gas station - by Bioz Stars, 2026-06
90/100 stars
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pyris  (Revvity)
91
Revvity pyris
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Pyris, supplied by Revvity, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
pyris - by Bioz Stars, 2026-06
91/100 stars
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plantscreen data analyzer software  (Photon Systems Instruments SRO)
90
Photon Systems Instruments SRO plantscreen data analyzer software
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Plantscreen Data Analyzer Software, supplied by Photon Systems Instruments SRO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
plantscreen data analyzer software - by Bioz Stars, 2026-06
90/100 stars
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peak analyzer function of origin 2018 software  (OriginLab corp)
90
OriginLab corp peak analyzer function of origin 2018 software
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Peak Analyzer Function Of Origin 2018 Software, supplied by OriginLab corp, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
peak analyzer function of origin 2018 software - by Bioz Stars, 2026-06
90/100 stars
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gelpro analyzer software version 3  (GraphPad Software Inc)
90
GraphPad Software Inc gelpro analyzer software version 3
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Gelpro Analyzer Software Version 3, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
gelpro analyzer software version 3 - by Bioz Stars, 2026-06
90/100 stars
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tga analyzing software  (TA Instruments)
90
TA Instruments tga analyzing software
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Tga Analyzing Software, supplied by TA Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
tga analyzing software - by Bioz Stars, 2026-06
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brain vision analyzer 2  (brain products gmbh)
90
brain products gmbh brain vision analyzer 2
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Brain Vision Analyzer 2, supplied by brain products gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
brain vision analyzer 2 - by Bioz Stars, 2026-06
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analyzing software rois  (Hamamatsu)
90
Hamamatsu analyzing software rois
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Analyzing Software Rois, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
analyzing software rois - by Bioz Stars, 2026-06
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land-1d analyzer software  (Beijing Sage Creation)
90
Beijing Sage Creation land-1d analyzer software
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Land 1d Analyzer Software, supplied by Beijing Sage Creation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
land-1d analyzer software - by Bioz Stars, 2026-06
90/100 stars
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nano analyze software ta instruments 10.1002/chem.201802248  (TA Instruments)
90
TA Instruments nano analyze software ta instruments 10.1002/chem.201802248
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
Nano Analyze Software Ta Instruments 10.1002/Chem.201802248, supplied by TA Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
nano analyze software ta instruments 10.1002/chem.201802248 - by Bioz Stars, 2026-06
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vision analyzer software  (brain products gmbh)
90
brain products gmbh vision analyzer software
(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad <t>TC20).</t> Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.
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(A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad TC20). Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.

Journal: Cell reports

Article Title: Mast cell-specific CysLT2 receptor signaling inhibits cysteinyl leukotriene-dependent mast cell activation and type 2 allergic lung inflammation

doi: 10.1016/j.celrep.2025.116758

Figure Lengend Snippet: (A) Protocol to induce allergic airway inflammation in Cysltr2 flox /Cpa3 cre (MC-specific Cysltr2 KO) mice and littermate controls. Df (1 μg) was administered intranasally on days 0, 4, 7, 11, 14, and 18. Euthanasia was performed 48 h after the last Df challenge. (B) Total/eosinophil cell counts and eosinophil (%) in BAL fluid of Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Total BAL cell numbers were counted by an automated cell counter (Bio-Rad TC20). Eosinophils were defined as CD45-positive/SiglecF-positive/CD11c-negative by flow cytometry. The percentage of eosinophils in the total BAL fluid cell population was calculated by comparing the eosinophil count to the total CD45 + cell count. (C and D) Total lung ILC2/β7+ MC counts and Il5 transcript expression in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Lung total cell numbers were determined using an automated cell counter (Bio-Rad TC20). (E) BAL fluid levels of PGD 2 and cysLTs (LTC 4 , LTD 4 , and LTE 4 ) in Df -treated Cre− (male, n = 8; female, n = 7) and Cre+ (male, n = 6; female, n = 8) on day 20. Values are mean ± SEM. * p < 0.05, by Mann-Whitney U test. (F–J) Similar analyses as in (A)–(E) were performed using female Cysltr2 flox /Cpa3 cre mice receiving vehicle (PBS) or CysLT 1 R antagonist montelukast (i.p. 30 mg/kg on days 0, 4, 7, 11, 14, and 18). Values are mean ± SEM. Df -treated Cre− (Veh, n = 14; montelukast, n = 11) and Cre+ (Veh, n = 14; montelukast, n = 11) from independent two experiments. * p < 0.05 compared to Cre −/PBS, # p < 0.05, ## p < 0.01 compared to Cre+/PBS, by two-way ANOVA with Sidak’s multiple comparisons test.

Article Snippet: TC20 TM Data Analyzer Software , BioRad , https://www.bio-rad.com/webroot/web/html/lsr/products/cell_counting/product_overlay/global/tc20-data-ana.html.

Techniques: Flow Cytometry, Cell Characterization, Expressing, MANN-WHITNEY