analyze software Search Results


tc20 data analyzer  (Bio-Rad)
99
Bio-Rad tc20 data analyzer
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Tc20 Data Analyzer, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/analyze+software/pmc11402648-115-0-4?v=Bio-Rad
Average 99 stars, based on 1 article reviews
tc20 data analyzer - by Bioz Stars, 2026-08
99/100 stars
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thermal analysis gas station  (Revvity)
90
Revvity thermal analysis gas station
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Thermal Analysis Gas Station, supplied by Revvity, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/analyze+software/10__4314_slash_tjpr__v13i1__4-61-17-21?v=Revvity
Average 90 stars, based on 1 article reviews
thermal analysis gas station - by Bioz Stars, 2026-08
90/100 stars
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analyzer software  (Meditron GmbH)
90
Meditron GmbH analyzer software
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Analyzer Software, supplied by Meditron GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/analyze+software/pmc01899182__1475___925X___6___16___S4-53-0-13?v=Meditron+GmbH
Average 90 stars, based on 1 article reviews
analyzer software - by Bioz Stars, 2026-08
90/100 stars
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analyzing software named  (ANSYS inc)
90
ANSYS inc analyzing software named
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Analyzing Software Named, supplied by ANSYS inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/analyze+software/us07539955-32-15-18?v=ANSYS+inc
Average 90 stars, based on 1 article reviews
analyzing software named - by Bioz Stars, 2026-08
90/100 stars
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online analyzer software  (SuperArray Bioscience Corporation)
90
SuperArray Bioscience Corporation online analyzer software
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Online Analyzer Software, supplied by SuperArray Bioscience Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/analyze+software/10__1161_slash_01__atv__0000196544__73761__82-112-5-5?v=SuperArray+Bioscience+Corporation
Average 90 stars, based on 1 article reviews
online analyzer software - by Bioz Stars, 2026-08
90/100 stars
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all-in-one fluorescence microscope software bz-h3a  (KEYENCE)
90
KEYENCE all-in-one fluorescence microscope software bz-h3a
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
All In One Fluorescence Microscope Software Bz H3a, supplied by KEYENCE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/analyze+software/pmc06917794__41467_2019_13640_MOESM3_ESM-8-8-16?v=KEYENCE
Average 90 stars, based on 1 article reviews
all-in-one fluorescence microscope software bz-h3a - by Bioz Stars, 2026-08
90/100 stars
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keyence analyzer  (KEYENCE)
90
KEYENCE keyence analyzer
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Keyence Analyzer, supplied by KEYENCE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/analyze+software/pmc08184845-144-6-6?v=KEYENCE
Average 90 stars, based on 1 article reviews
keyence analyzer - by Bioz Stars, 2026-08
90/100 stars
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bz-analyzer software  (KEYENCE)
90
KEYENCE bz-analyzer software
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Bz Analyzer Software, supplied by KEYENCE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/analyze+software/pmc08534131-60-15-17?v=KEYENCE
Average 90 stars, based on 1 article reviews
bz-analyzer software - by Bioz Stars, 2026-08
90/100 stars
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analyzing application software vk analyzer  (KEYENCE)
90
KEYENCE analyzing application software vk analyzer
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Analyzing Application Software Vk Analyzer, supplied by KEYENCE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/analyze+software/us08703346-492-29-34?v=KEYENCE
Average 90 stars, based on 1 article reviews
analyzing application software vk analyzer - by Bioz Stars, 2026-08
90/100 stars
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analyzer software  (Ionoptika Ltd)
90
Ionoptika Ltd analyzer software
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Analyzer Software, supplied by Ionoptika Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/analyze+software/pmc11428081-44-7-9?v=Ionoptika+Ltd
Average 90 stars, based on 1 article reviews
analyzer software - by Bioz Stars, 2026-08
90/100 stars
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image-analyzer software  (KEYENCE)
90
KEYENCE image-analyzer software
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Image Analyzer Software, supplied by KEYENCE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/analyze+software/pmc11472835-72-4-4?v=KEYENCE
Average 90 stars, based on 1 article reviews
image-analyzer software - by Bioz Stars, 2026-08
90/100 stars
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bzii-analyzer software  (KEYENCE)
90
KEYENCE bzii-analyzer software
PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad <t>TC20</t> Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).
Bzii Analyzer Software, supplied by KEYENCE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/analyze+software/pmc11537973-123-19-22?v=KEYENCE
Average 90 stars, based on 1 article reviews
bzii-analyzer software - by Bioz Stars, 2026-08
90/100 stars
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Image Search Results


PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad TC20 Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).

Journal: iScience

Article Title: A feedback loop between plakophilin 4 and YAP signaling regulates keratinocyte differentiation

doi: 10.1016/j.isci.2024.110762

Figure Lengend Snippet: PKP4 modulates keratinocyte proliferation (A) Levels of PKP4 protein in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h or 72h in medium with or without Ca 2+ . Left: Representative western blots of PKP4. GAPDH was used as a loading control. Right: Quantification of PKP4 protein amounts normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. (B) Measurement of proliferation using an IncuCyte S3 system. Left: Representative live cell images of WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for up to 96h in LCM. Scale bar = 100 μm. Right: Area occupied by cell nuclei at the indicated time points, as determined from live cell images. Data are shown relative to measurements at the beginning of recording (time zero). Graphs represent average values from three independent experiments. (C) The diameter of trypsinized WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in LCM automatically measured using the BioRad TC20 Cell Counter. Top: Representative live cell images. Scale bar = 100 μm. Bottom: Quantification of cell size. Averages +SD from three independent experiments are plotted. n ≥ 1,000 cells per condition. (D) Propidium iodide-labeling and flow cytometry analysis of the cell cycle. Number of WT and PKP4-KO cells grown for 24h in LCM or HCM in G0/G1 phase, S phase, or G2/M phase. Averages +SD from three independent experiments are plotted. (E) BrdU incorporation in WT and PKP4-KO cells grown for 24h, 48h, or 72h in LCM or HCM. Averages +SD from three independent experiments are plotted. (F) Levels of proliferation markers in WT, PKP4-KO, and PKP4-KO+PKP4 (Rescue) cells grown for 24h in medium with or without Ca 2+ . Left: Schematic of cell cycle dependent expression of RB and H3. Since RB is phosphorylated in late G1 phase, thereby releasing E2F1 and promoting its transcriptional activity, phospho-RB was used as a marker for G1-S phase transition. Phosphorylation of H3 served as an M phase marker. Mid: Representative western blots of phospho-RB and phospho-H3. GAPDH was used as a loading control. Right: Quantification of phosphorylation levels normalized to GAPDH and relative to WT cells grown in medium without Ca 2+ . Averages +SD from three independent experiments are plotted. Quantification of LCM versus HCM treatment has been omitted. ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ns, not significant. Significance was determined by one-way ANOVA with Tukey’s multiple comparisons test (A, B, C, and F) or by student’s unpaired two-tailed t-test (D and E).

Article Snippet: TC20 data analyzer , BioRad , https://www.bio-rad.com/webroot/web/html/lsr/products/cell_counting/product_overlay/global/tc20-data-ana.html.

Techniques: Western Blot, Control, Labeling, Flow Cytometry, BrdU Incorporation Assay, Expressing, Activity Assay, Marker, Sublimation, Phospho-proteomics, Two Tailed Test

Journal: iScience

Article Title: A feedback loop between plakophilin 4 and YAP signaling regulates keratinocyte differentiation

doi: 10.1016/j.isci.2024.110762

Figure Lengend Snippet:

Article Snippet: TC20 data analyzer , BioRad , https://www.bio-rad.com/webroot/web/html/lsr/products/cell_counting/product_overlay/global/tc20-data-ana.html.

Techniques: Transduction, Virus, Recombinant, Formulation, Random Hexamer, Reverse Transcription, Western Blot, Enzyme-linked Immunosorbent Assay, Bicinchoninic Acid Protein Assay, Expressing, Mutagenesis, Plasmid Preparation, Software