Journal: Scientific Reports
Article Title: The Statistical Value of Raw Fluorescence Signal in Luminex xMAP Based Multiplex Immunoassays
Figure Lengend Snippet: Statistical model for analyte expression. ( a ) Two linear mixed-effects models in R-notation. Main fixed effects are Cytokine, 37 levels, Tissue, 4 levels, and Condition with 8 levels. ( b ) Gives the results of a statistical comparison of the reduced model against the global model. AIC Akaike’s Information Criterion, BIC Bayesian Information Criterion, and the smaller they are the better the model fit. Comparisons between reduced, ( c ), and global mixed-effects models ( d ) for selected analytes using regression conditional plots. Conditional plots show the relationship between the outcome and explanatory/conditional variables to be viewed as other effects are held constant. Note for the global model, ( d ), the cluster of points (residuals) around each conditional mean response (dark horizontal line) is tighter than that seen in the reduced model, ( c ). Both models contain the same number of samples per tissue. For brevity, only results for 9 of the 37 analytes are given, however, the same comparison but for all 37 analytes are given in Supplementary Fig. S2 . Since the conditional residuals are reasonably scattered above and below their respective means, implies that either model is a reasonable fit to the data.
Article Snippet: The fluorescence responses and concentrations of analytes were obtained using a Bio-Plex Pro™ Human Inflammation Panel 37-Plex assay kit with magnetic beads (171AL1001M, Bio-Rad, Hercules, California, USA) and analysed with a Luminex100 system and the accompanying Bio-Plex ManagerTM Software 6.1(Bio-Rad, Hercules, California, USA).