Journal: International Journal of Pharmacokinetics

Article Title: Method development challenges and regulatory expectation in nifedipine

doi: 10.4155/ipk-2016-0004

Figure Lengend Snippet: Figure 3. Matrix effect: postcolumn infusion (T-Joint) method for nifedipine and nifedipine–d4 (RT 2.88) to demonstrate absence of any ion suppression/enhancement.

Article Snippet: Dehydro nifedipine (purity: 98%) and hydroxydehydro nifedipine carboxylate (purity: 97%) were procured from Toronto Research Chemicals (Ontario, Canada).

Techniques:

Journal: Computers in Biology and Medicine

Article Title: Identification of FDA approved drugs and nucleoside analogues as potential SARS-CoV-2 A1pp domain inhibitor: An in silico study

doi: 10.1016/j.compbiomed.2020.104185

Figure Lengend Snippet: Surface structure of lead FDA approved drug, nucleoside inhibitor and natural substrate at SARS-CoV-2 A1pp domain active site. (A) Surface structure of lactobionic acid at ADP-ribose-1″-phosphatase active site. (B) Surface structure of NA 1 at ADP-ribose-1″-phosphatase active site. (C) Surface structure of adenosine-5-diphosphoribose at ADP-ribose-1″-phosphatase active site. (D) 2D structure of lactobionic acid. (E) 2D structure of NA 1 . (F) 2D structure of adenosine-5-diphosphoribose.

Article Snippet: The structures of FDA approved drugs and nucleoside analogues were downloaded from selleckchem ( https://www.selleckchem.com/ ) in. sdf format and converted to different formats using Open Babel software.

Techniques:

Journal: Autophagy

Article Title: RNF186 regulates EFNB1 (ephrin B1)-EPHB2-induced autophagy in the colonic epithelial cells for the maintenance of intestinal homeostasis

doi: 10.1080/15548627.2020.1851496

Figure Lengend Snippet: EFNB1-induced autophagy is independent of MTOR inhibition. (A) Ls174t cells were treated as indicated, followed by western blot analysis of the indicated proteins. Densitometry quantification from three independent experiments was shown in the right panel. (B-D) Ls174t cells were pretreated with SBI-0206965 (10 μM) (B), 3-MA (5 mM) (C) or wortmannin (100 nM) (D) for 1 h, followed by the treatment of plate-coated Fc or ephrin-B1-Fc (10 ug/ml) in presence of the inhibitors for the indicated time. Cell lysates were analyzed by western blot for the indicated proteins. Densitometry quantification from three independent experiments was shown in the right panel. (E) Ls174t cells were pretreated with DMSO, 3-MA (5 mM) or wortmannin (100 nM) for 1 h, followed by plate-coated Fc or ephrin-B1-Fc (10 μg/ml) for 2 h in presence of the inhibitors and followed by confocal microscopy analysis of MAP1LC3B puncta. Quantification of cells with puncta of MAP1LC3B-II was shown in the bottom panel. Scale bar: 5 μm. (F) Ls174t cells were pretreated with VPS34-IN1 (2 μM) for 1 h, followed by the treatment of plate-coated Fc or ephrin-B1-Fc (10 ug/ml) in presence of the inhibitor for the indicated time. Cell lysates were analyzed by western blot for the indicated proteins. Densitometry quantification was shown in the right panel. (G) Control-gRNA or ATG5-gRNA infected Ls174t cells were treated with 10 μg/ml plate-coated Fc (0 time point) or ephrin-B1-Fc, followed by western blot analysis of the indicated proteins. Densitometry quantification from three independent experiments was shown in the right panel. WT: wild type; KO: knockout; 3-MA: 3-methyladenine. All error bars represent SEM of technical replicates. *: P < 0.05, **: P < 0.01, ***: P < 0.001, ****: P < 0.0001 based on two-sided unpaired T test (A-G). Densitometry quantification of A-D and F-G were based on three independent experiments. Data are representative of three independent experiments

Article Snippet: ULK inhibitor SBI-0206965 (T2128), PtdIns3K inhibitor 3-MA (T1879), wortmannin (T6283) and Vps34-IN-1 (T7015) were bought from TargetMol.

Techniques: Inhibition, Western Blot, Confocal Microscopy, Infection, Knock-Out

Journal: Cell stem cell

Article Title: Jak1 Integrates Cytokine Sensing to Regulate Hematopoietic Stem Cell Function and Stress Hematopoiesis

doi: 10.1016/j.stem.2017.08.011

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: To test the effect of pharmacological Jak1 or Cdk6 inhibition, whole BM cells from wt mice were plated with varying concentrations of GLPG0634 or PD332991 (Selleckchem).

Techniques: Recombinant, Microscopy, Software

Journal: bioRxiv

Article Title: CRISPRa screen identifies a role for c-KIT signaling in tamoxifen resistance, potentially through upregulation of ABC transporters

doi: 10.1101/2022.08.22.504845

Figure Lengend Snippet: A) The percentage of inhibition of MCF-7-S cells treated with increasing concentrations of TAM and Gleevec. The shade of red represents the amount of inhibition in cells. B) Observed synergism scores that correspond to the percent inhibition values of TAM and Gleevec treatment using ZIP synergy from SynergyFinder 2.0.

Article Snippet: The following cell culture media supplements and concentrations were used throughout this study unless indicated otherwise: Doxycycline hyclate (2ug/ml, Sigma, cat# D9891-5G), tamoxifen as (Z)-4-Hydroxytamoxifen (1uM, 4-OHT; Sigma-Aldrich; Cat# H7904), and Gleevec (Imatinib mesylate; Santa Cruz; cat # CAS 220127-57-1).

Techniques: Inhibition