Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Uterine Foxl2 regulates the adherence of the Trophectoderm cells to the endometrial epithelium

doi: 10.1186/s12958-018-0329-y

Figure Lengend Snippet: Foxl2 expression in uterine cell lines. qRT-PCR of Foxl2 mRNA expression in AN3-CA and Ishikawa endometrial cell lines. Endogenous expression in Ishikawa cells is significantly lower

Article Snippet: The AN3-CA cells, non-receptive human endometrial cells (cell line obtained from CLS Cell Line Services GmbH, Eppelheim, Germany), were grown in MEM (Biological Industries, Israel) with 10% fetal bovine serum (FBS, Hyclone, Biological Industries, Israel), Ishikawa cells, receptive human endometrial cells (cell line obtained from ATCC, Manassas, VA), were grown in DMEM (Biological Industries, Israel) with 10% FBS.

Techniques: Expressing, Quantitative RT-PCR

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Uterine Foxl2 regulates the adherence of the Trophectoderm cells to the endometrial epithelium

doi: 10.1186/s12958-018-0329-y

Figure Lengend Snippet: Foxl2 effects in an in vitro model of attachment. a Mouse embryo attachment is higher after Foxl2 knockdown in AN3-CA cells. b Overexpression of Foxl2 in Ishikawa cells reduces mouse embryo attachment. c Jeg3 spheroid attachment is higher after Foxl2 knockdown in AN3-CA. d Overexpression of Foxl2 in Ishikawa cells decreases Jeg3 spheroid attachment. * p < 0.05

Article Snippet: The AN3-CA cells, non-receptive human endometrial cells (cell line obtained from CLS Cell Line Services GmbH, Eppelheim, Germany), were grown in MEM (Biological Industries, Israel) with 10% fetal bovine serum (FBS, Hyclone, Biological Industries, Israel), Ishikawa cells, receptive human endometrial cells (cell line obtained from ATCC, Manassas, VA), were grown in DMEM (Biological Industries, Israel) with 10% FBS.

Techniques: In Vitro, Knockdown, Over Expression

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Uterine Foxl2 regulates the adherence of the Trophectoderm cells to the endometrial epithelium

doi: 10.1186/s12958-018-0329-y

Figure Lengend Snippet: Foxl2 effect on the expression of Wnt/Fzd family members. a , d , g The expression of Fzd6 and Wnt11 is higher, while the expression of Kremen2 , a Wnt/Fzd family inhibitor, is lower in receptive endometrial Ishikawa cells, which express low Foxl2 levels, compared to AN3-CA endometrial non-receptive cells, which express higher levels of Foxl2 . b , e , h Knockdown of Foxl2 in AN3-CA leads to an increase in Fzd6 and Wnt11 expression and a reduction in Kremen2 levels. c , f , i Overexpression of Foxl2 in Ishikawa cells leads to a decrease in Fzd6 and Wnt11 levels but elevates Kremen2 levels. * p < 0.05

Article Snippet: The AN3-CA cells, non-receptive human endometrial cells (cell line obtained from CLS Cell Line Services GmbH, Eppelheim, Germany), were grown in MEM (Biological Industries, Israel) with 10% fetal bovine serum (FBS, Hyclone, Biological Industries, Israel), Ishikawa cells, receptive human endometrial cells (cell line obtained from ATCC, Manassas, VA), were grown in DMEM (Biological Industries, Israel) with 10% FBS.

Techniques: Expressing, Knockdown, Over Expression

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Uterine Foxl2 regulates the adherence of the Trophectoderm cells to the endometrial epithelium

doi: 10.1186/s12958-018-0329-y

Figure Lengend Snippet: Foxl2 effect on the expression of genes involved in apoptosis. a , d , g The expression of Tnfip3 and Atf3 is elevated, while the expression of Ier3 is decreased, in receptive endometrial Ishikawa cells, expressing low Foxl2 levels as compared to AN3-CA, endometrial non-receptive cells, expressing high levels of Foxl2 . b , e , h Tnfip3 and Atf3 expression is reduced, while the expression of Ier3 is elevated in AN3-CA after Foxl2 knockdown. c , f , i Overexpression of Foxl2 in Ishikawa cells is associated with a decrease in Tnfip3 and Atf3 levels and increase in Ier3 levels. * p < 0.05

Article Snippet: The AN3-CA cells, non-receptive human endometrial cells (cell line obtained from CLS Cell Line Services GmbH, Eppelheim, Germany), were grown in MEM (Biological Industries, Israel) with 10% fetal bovine serum (FBS, Hyclone, Biological Industries, Israel), Ishikawa cells, receptive human endometrial cells (cell line obtained from ATCC, Manassas, VA), were grown in DMEM (Biological Industries, Israel) with 10% FBS.

Techniques: Expressing, Knockdown, Over Expression

Journal: Reproductive Biology and Endocrinology : RB&E

Article Title: Uterine Foxl2 regulates the adherence of the Trophectoderm cells to the endometrial epithelium

doi: 10.1186/s12958-018-0329-y

Figure Lengend Snippet: Foxl2 effect on the expression of genes involved in embryo implantation. The expression of Rgs2 and Cxcl1 is elevated in receptive endometrial Ishikawa cells, expressing low Foxl2 levels as compared to AN3-CA, endometrial non-receptive cells, expressing high levels of Foxl2 , ( a , d ) as well as in AN3-CA Foxl2 depleted cells ( b , e ). Overexpression of Foxl2 in Ishikawa cells resulted in a decrease in Rgs2 and Cxcl1 levels ( c , f ). * p < 0.05

Article Snippet: The AN3-CA cells, non-receptive human endometrial cells (cell line obtained from CLS Cell Line Services GmbH, Eppelheim, Germany), were grown in MEM (Biological Industries, Israel) with 10% fetal bovine serum (FBS, Hyclone, Biological Industries, Israel), Ishikawa cells, receptive human endometrial cells (cell line obtained from ATCC, Manassas, VA), were grown in DMEM (Biological Industries, Israel) with 10% FBS.

Techniques: Expressing, Over Expression

Journal: Oncology Research

Article Title: Regulation of Histone Emulsification by HPDL via LDHA/LDHB Promotes EC Cell Proliferation

doi: 10.32604/or.2026.068833

Figure Lengend Snippet: Knockdown of HPDL decreased the level of histone lactonization modification in EC cells. ( A ) Western blot (WB) analysis confirming the expression of histone lactate dehydrogenase (HLD) antibodies in AN3CA cells with HPDL knockdown; ( B ) WB analysis confirming the expression of HLD antibodies in HEC-1-B cells with HPDL knockdown; ( C ) Quantitative polymerase chain reaction (qPCR) analysis of HPDL expression following triple-sequence plasmid knockdown in AN3CA cells; ( D ) qPCR analysis of HPDL expression following triple-sequence plasmid knockdown in HEC-1-B cells; ( E ) Measurement of lactate content in AN3CA cells subsequent to HPDL knockdown; ( F ) Measurement of lactate content in HEC-1-B cells subsequent to HPDL knockdown. ( G ) Green fluorescence signifies HPDL protein expression, while blue fluorescence indicates immunofluorescence staining of cell nuclei; scale bar is 50 μm; ( H ) Expression levels of HPDL, Pan Kla, and H3K18la after the addition of varying concentrations of sodium lactate to AN3CA Si HPDL knockdown cell lines; scale bar represents 50 μm. Expression levels of HPDL, Pan Kla, and H3K18la in AN3CA Si HPDL knockdown cell lines with the addition of varying concentrations of sodium lactate; scale bar represents 50 μm; ( I ) Expression levels of HPDL, Pan Kla, and H3K18la after the addition of varying concentrations of sodium lactate to AN3CA normal cell lines. *indicates p < 0.05, **indicates p < 0.01, ***indicates p < 0.001

Article Snippet: AN3CA cell line , Procell , CL-0530.

Techniques: Knockdown, Modification, Western Blot, Expressing, Real-time Polymerase Chain Reaction, Sequencing, Plasmid Preparation, Fluorescence, Immunofluorescence, Staining

Journal: Oncology Research

Article Title: Regulation of Histone Emulsification by HPDL via LDHA/LDHB Promotes EC Cell Proliferation

doi: 10.32604/or.2026.068833

Figure Lengend Snippet: The level of histone lactylation was elevated in EC cells overexpressing HPDL ( A ) Expression levels of HPDL, Pan Kla, and H3K18la following the overexpression of HPDL in the HEC-1-B cell line; ( B ) Expression levels of HPDL, Pan Kla, and H3K18la after treating the HEC-1-B cell LV-HPDL with varying concentrations of sodium oxalate; ( C ) Overexpression and knockdown strains of AN3CA; ( D ) Lactate levels in HEC-1-B overexpression and knockdown strains; ( E ) Immunofluorescence of AN3CA cells for quantifying H3K18la immunofluorescence across different groups; ( F ) Immunofluorescence of AN3CA cells for quantifying DAPI immunofluorescence across different groups; ( G ) Immunofluorescence of AN3CA cells for quantifying the expression of H3K18la and DAPI, scale bar is 50 μm; *indicating p < 0.05, **indicating p < 0.01, ***indicating p < 0.001, and ns indicating no statistical significance

Article Snippet: AN3CA cell line , Procell , CL-0530.

Techniques: Expressing, Over Expression, Knockdown, Immunofluorescence

Journal: Oncology Research

Article Title: Regulation of Histone Emulsification by HPDL via LDHA/LDHB Promotes EC Cell Proliferation

doi: 10.32604/or.2026.068833

Figure Lengend Snippet: The expression of HPDL promotes the proliferation of EC cells and affects the cell cycle. ( A ) CCK-8 profiles in AN3CA cells; ( B ) CCK-8 profiles in HEC-1-B cells; ( C ) Quantitative results of plate cloning assay of HEC-1-B cells; ( D ) Plate cloning assay of AN3CA and HEC-1-B cells; ( E ) Quantification results of plate cloning of AN3CA cells; ( F ) AN3CA cell cycle assay, *indicating p < 0.05 and ***indicating p < 0.001

Article Snippet: AN3CA cell line , Procell , CL-0530.

Techniques: Expressing, CCK-8 Assay, Cloning, Cell Cycle Assay

Journal: Oncology Research

Article Title: Regulation of Histone Emulsification by HPDL via LDHA/LDHB Promotes EC Cell Proliferation

doi: 10.32604/or.2026.068833

Figure Lengend Snippet: HPDL promotes the migration and invasion of EC cells. ( A ) Cell scratch assay of the normal, overexpression and knockdown groups of the HPDL gene in AN3CA cells, scale bar is 50 μm; ( B ) Cell scratch assay of the normal, overexpression and knockdown groups of the HPDL gene in HEC-1-B cells; ( C ) AN3CA and HEC-1-B cells with the normal, overexpression and knockdown groups of the HPDL gene in the quantitative histograms of cell scratch assay cells; ( D ) quantitative histograms of Transwell migration assay of AN3CA cells; ( E ) quantitative histograms of Transwell migration assay of HEC-1-B cells; ( F ) Transwell migration assay of AN3CA and HEC-1-B cells, scale bar is 50 μm; ( G ) AN3CA and HEC-1-B cells Transwell invasion assay; ( H ) AN3CA and HEC-1-B cell HPDL overexpression Transwell invasion assay; ( I ) Transwell invasion assay with HPDL knockdown in AN3CA and HEC-1-B cells, ***indicating p < 0.001

Article Snippet: AN3CA cell line , Procell , CL-0530.

Techniques: Migration, Wound Healing Assay, Over Expression, Knockdown, Transwell Migration Assay, Transwell Invasion Assay

Journal: Oncology Research

Article Title: Regulation of Histone Emulsification by HPDL via LDHA/LDHB Promotes EC Cell Proliferation

doi: 10.32604/or.2026.068833

Figure Lengend Snippet: HPDL promotes histone lactylation levels in EC cells by upregulating LDHA/LDHB expression. ( A ) Chordal graph of GO/KEGG analysis; ( B ) Western blot (WB) demonstrates detection of Pan Kla, H3K18la antibody expression after LDHA and LDHB inhibition; ( C ) Reverse transcription-quantitative polymerase chain reaction (qPCR) showing the expression of HPDL in AN3CA cells with SiNC, SiLDHA, and SiLDHB; ( D ) WB demonstration of fluorescence transfection efficiency and LDHA/LDHB and HPDL expression in AN3CA cells with SiNC, SiLDHA, and SiLDHB; ( E ) qPCR of AN3CA cells with decreased LDHA/LDHB expression in the SiHPDL group, and decreased LDHA/LDHB expression in the LVHPDL group, where LDHA/LDHB expression increased; ( F) H3K18la expression in the LDHA promoter region in AN3CA cells with SiNC, LV-HPDL, and LV-HPDL+Oxmate; ( G ) H3K18la expression in the LDHB promoter region in AN3CA cells with SiNC, LV-HPDL, and LV-HPDL+Oxmate; ( H ) H3K18la expression in the LDHA promoter region in AN3CA cells with SiNC, SiHPDL, and SiHPDL+L-NaLa; ( I ) H3K18la expression in the LDHB promoter region in AN3CA cells with SiNC, SiHPDL, and SiHPDL+L-NaLa; *indicates p < 0.05, **indicates p < 0.01, ***indicates p < 0.001

Article Snippet: AN3CA cell line , Procell , CL-0530.

Techniques: Expressing, Western Blot, Inhibition, Reverse Transcription, Real-time Polymerase Chain Reaction, Fluorescence, Transfection