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  • 99
    Thermo Fisher amplitaq gold dna polymerase
    Hot Start <t>DNA</t> polymerase evaluation in triplex one-step RT-PCR amplification of ABCA5, ABCA6 and ABCA7 targets . Reactions contained 0.82 μg of human trachea total RNA and an unmodified oligo(dT) 18 RT primer. The PCR primers were either unmodified, or contained CleanAmp™ Precision modifications. These reactions contained one of the following DNA polymerases: Taq , Platinum ® Taq , or <t>AmpliTaq</t> Gold ® and one of the following reverse transcriptases: M-MLV or SSIII. Reactions with M-MLV were incubated at 42°C, while reactions with SSIII were incubated at 55°C.
    Amplitaq Gold Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 33231 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 33231 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold dna polymerase - by Bioz Stars, 2020-09
    99/100 stars
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    99
    Thermo Fisher amplitaq gold 360 dna polymerase
    Inhibition and the effect of hot-start. ( a ) Inhibition profiles of 2D9 versus <t>AmpliTaq</t> <t>Gold</t> 360 (ATaqG) in PCR. The effect of various inhibitors (SP61: bone dust, Tar, Soil, 1736; cave sediment) on polymerase activity of 2D9 and ATaqG as determined by a PCR assay in the presence of decreasing amounts of inhibitor and plotted as the average relative resistance ( n = 3, standard error) (as in Figure 4 ) of 2D9 and ATaqG with respect to ATaqG as a reference (resistance = 1). ( b ) The effect of manual hot-start on resistance of 2D9 polymerase to inhibitors tar and soil at minimal limiting amounts of inhibitor. Hot-start increases inhibitor resistance by at least a factor of two.
    Amplitaq Gold 360 Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 529 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold 360 dna polymerase/product/Thermo Fisher
    Average 99 stars, based on 529 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold 360 dna polymerase - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

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    Hot Start DNA polymerase evaluation in triplex one-step RT-PCR amplification of ABCA5, ABCA6 and ABCA7 targets . Reactions contained 0.82 μg of human trachea total RNA and an unmodified oligo(dT) 18 RT primer. The PCR primers were either unmodified, or contained CleanAmp™ Precision modifications. These reactions contained one of the following DNA polymerases: Taq , Platinum ® Taq , or AmpliTaq Gold ® and one of the following reverse transcriptases: M-MLV or SSIII. Reactions with M-MLV were incubated at 42°C, while reactions with SSIII were incubated at 55°C.

    Journal: BMC Molecular Biology

    Article Title: Selective control of primer usage in multiplex one-step reverse transcription PCR

    doi: 10.1186/1471-2199-10-113

    Figure Lengend Snippet: Hot Start DNA polymerase evaluation in triplex one-step RT-PCR amplification of ABCA5, ABCA6 and ABCA7 targets . Reactions contained 0.82 μg of human trachea total RNA and an unmodified oligo(dT) 18 RT primer. The PCR primers were either unmodified, or contained CleanAmp™ Precision modifications. These reactions contained one of the following DNA polymerases: Taq , Platinum ® Taq , or AmpliTaq Gold ® and one of the following reverse transcriptases: M-MLV or SSIII. Reactions with M-MLV were incubated at 42°C, while reactions with SSIII were incubated at 55°C.

    Article Snippet: Reaction conditions included 1× PCR buffer (20 mM Tris (pH 8.4), 50 mM KCl) (Invitrogen), 1.5 mM MgCl2 (Invitrogen), gene-specific PCR primers (0.5 μM) (TriLink), oligo(dT)18 primer (1 μM) (TriLink), 0.16 mM dNTPs (New England Biolabs), 0.5 μL of human trachea total RNA or other human total RNA (Stratagene or Ambion, each at ~1.6 μg/μL), 5 U RNase Inhibitor (New England Biolabs), 50 U Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) (Invitrogen) or SuperScript® III Reverse Transcriptase (SSIII RT) (Invitrogen), and 0.6 U of Taq DNA Polymerase, recombinant (Invitrogen) or Platinum® Taq DNA Polymerase (Invitrogen) or AmpliTaq Gold® DNA Polymerase (Applied Biosystems), in a 50 μL reaction volume.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Polymerase Chain Reaction, Incubation

    Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for AmpliTaq Gold and “Platin” for Platinum Taq High Fidelity.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Patterns of nucleotide misincorporations during enzymatic amplification and direct large-scale sequencing of ancient DNA

    doi: 10.1073/pnas.0605327103

    Figure Lengend Snippet: Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for AmpliTaq Gold and “Platin” for Platinum Taq High Fidelity.

    Article Snippet: These oligonucleotides were used as templates in PCRs with two DNA polymerase reagents, AmpliTaq Gold (Applied Biosystems, Foster City, CA) and Platinum Taq High Fidelity (Invitrogen, Carlsbad, CA), which are both commonly used in ancient DNA research.

    Techniques: Clone Assay, Amplification, Generated

    Nested-PCR assay. (A) MluI qPCR assay to detect pXMRV1 contamination. The 5′ end of the probe spans the MluI restriction site that was introduced to create pXMRV1. pAO-H4, which does not have the MluI restriction site, has lower peak fluorescence as well as a delay in C T s for the same copy numbers of plasmid. (B) Sensitivity of the IAP qPCR assay for different amounts of C57BL/6 mouse DNA ranging from 62.5 ng to 625 ag, all in the presence of 400 ng of human placental DNA. (C) Nested-PCR assay of a small set of samples, showing ∼5% of the samples to be positive for MLV gag ). LNCaP genomic DNA is shown in lanes G. The lane labeled “−” represents the negative control. Lane l shows the 100-bp ladder. (D) Detection of mouse DNA in Platinum Taq (IP Taq, Invitrogen) and Recombinant Taq (IR Taq, Invitrogen) but not in AmpliTaq Gold (AAG Taq, Applied Biosystems). For each qPCR assay, the left column shows the number of replicates that are positive, and the right column shows the average C T at which positivity occurred. The more-XMRV-specific pol qPCR assay (in triplicate) was consistently negative, but IAP and gag assays (eight replicates each) were both positive, as more Platinum or Recombinant Invitrogen Taq was used as a template.

    Journal: Journal of Virology

    Article Title: Absence of XMRV Retrovirus and Other Murine Leukemia Virus-Related Viruses in Patients with Chronic Fatigue Syndrome ▿Absence of XMRV Retrovirus and Other Murine Leukemia Virus-Related Viruses in Patients with Chronic Fatigue Syndrome ▿ ¶

    doi: 10.1128/JVI.00693-11

    Figure Lengend Snippet: Nested-PCR assay. (A) MluI qPCR assay to detect pXMRV1 contamination. The 5′ end of the probe spans the MluI restriction site that was introduced to create pXMRV1. pAO-H4, which does not have the MluI restriction site, has lower peak fluorescence as well as a delay in C T s for the same copy numbers of plasmid. (B) Sensitivity of the IAP qPCR assay for different amounts of C57BL/6 mouse DNA ranging from 62.5 ng to 625 ag, all in the presence of 400 ng of human placental DNA. (C) Nested-PCR assay of a small set of samples, showing ∼5% of the samples to be positive for MLV gag ). LNCaP genomic DNA is shown in lanes G. The lane labeled “−” represents the negative control. Lane l shows the 100-bp ladder. (D) Detection of mouse DNA in Platinum Taq (IP Taq, Invitrogen) and Recombinant Taq (IR Taq, Invitrogen) but not in AmpliTaq Gold (AAG Taq, Applied Biosystems). For each qPCR assay, the left column shows the number of replicates that are positive, and the right column shows the average C T at which positivity occurred. The more-XMRV-specific pol qPCR assay (in triplicate) was consistently negative, but IAP and gag assays (eight replicates each) were both positive, as more Platinum or Recombinant Invitrogen Taq was used as a template.

    Article Snippet: Applied Biosystems' AmpliTaq Gold Taq polymerase contained in the master mix of all of our qPCR TaqMan assays did not contain any IAP sequences ( D), indicating that it was free of mouse DNA.

    Techniques: Nested PCR, Real-time Polymerase Chain Reaction, Fluorescence, Plasmid Preparation, Labeling, Negative Control, Recombinant

    HLA-PCR SBT with R660V and AmpliTaq Gold. PCR amplification of six DNAs using either KlenTaq R660V (A) or AmpliTaq Gold (B). The HLA-DRB1 profiles of the genomic DNA are: lane 1: HLA-DRB1* 13∶01∶01, 13∶02∶01; lane 2: HLA-DRB1* 03∶01∶01, 11∶01∶01; lane 3: HLA-DRB1 01∶01∶01, 13∶01∶01; lane 4: HLA-DRB1* 08∶01∶03, 08∶01∶03; lane 5: HLA-DRB1* 01∶01∶01, 07∶01∶01; lane 6: HLA-DRB1* 07∶01∶01, 15∶01∶01.

    Journal: PLoS ONE

    Article Title: Variants of a Thermus aquaticus DNA Polymerase with Increased Selectivity for Applications in Allele- and Methylation-Specific Amplification

    doi: 10.1371/journal.pone.0096640

    Figure Lengend Snippet: HLA-PCR SBT with R660V and AmpliTaq Gold. PCR amplification of six DNAs using either KlenTaq R660V (A) or AmpliTaq Gold (B). The HLA-DRB1 profiles of the genomic DNA are: lane 1: HLA-DRB1* 13∶01∶01, 13∶02∶01; lane 2: HLA-DRB1* 03∶01∶01, 11∶01∶01; lane 3: HLA-DRB1 01∶01∶01, 13∶01∶01; lane 4: HLA-DRB1* 08∶01∶03, 08∶01∶03; lane 5: HLA-DRB1* 01∶01∶01, 07∶01∶01; lane 6: HLA-DRB1* 07∶01∶01, 15∶01∶01.

    Article Snippet: Reagents and Instruments Oligonucleotides were purchased from Biomers or Metabion, HeLa genomic DNA and Taq 2x master mix was bought from New England Biolabs, dNTPs were either from Roche or Fermentas, Phusion DNA polymerase was purchased from Thermo Scientific, Platinum Taq and AmpliTaq Gold DNA polymerases from Life Technologies, DNase I, SphI, and HindIII from Fermentas, the Gel Extraction and EpiTect MSP kit from Qiagen and used according to their manuals.

    Techniques: Polymerase Chain Reaction, Amplification

    Inhibition and the effect of hot-start. ( a ) Inhibition profiles of 2D9 versus AmpliTaq Gold 360 (ATaqG) in PCR. The effect of various inhibitors (SP61: bone dust, Tar, Soil, 1736; cave sediment) on polymerase activity of 2D9 and ATaqG as determined by a PCR assay in the presence of decreasing amounts of inhibitor and plotted as the average relative resistance ( n = 3, standard error) (as in Figure 4 ) of 2D9 and ATaqG with respect to ATaqG as a reference (resistance = 1). ( b ) The effect of manual hot-start on resistance of 2D9 polymerase to inhibitors tar and soil at minimal limiting amounts of inhibitor. Hot-start increases inhibitor resistance by at least a factor of two.

    Journal: Nucleic Acids Research

    Article Title: Molecular breeding of polymerases for resistance to environmental inhibitors

    doi: 10.1093/nar/gkq1360

    Figure Lengend Snippet: Inhibition and the effect of hot-start. ( a ) Inhibition profiles of 2D9 versus AmpliTaq Gold 360 (ATaqG) in PCR. The effect of various inhibitors (SP61: bone dust, Tar, Soil, 1736; cave sediment) on polymerase activity of 2D9 and ATaqG as determined by a PCR assay in the presence of decreasing amounts of inhibitor and plotted as the average relative resistance ( n = 3, standard error) (as in Figure 4 ) of 2D9 and ATaqG with respect to ATaqG as a reference (resistance = 1). ( b ) The effect of manual hot-start on resistance of 2D9 polymerase to inhibitors tar and soil at minimal limiting amounts of inhibitor. Hot-start increases inhibitor resistance by at least a factor of two.

    Article Snippet: Inhibition profiles for AmpliTaq Gold 360 DNA polymerase (Applied Biosystems) were determined using an identical PCR set-up but using AmpliTaq Gold buffer and thermocycling conditions according to manufacturer’s recommendations.

    Techniques: Inhibition, Polymerase Chain Reaction, Activity Assay