amplitaq Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher amplitaq gold360
    Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for <t>AmpliTaq</t> Gold and “Platin” for Platinum Taq High Fidelity.
    Amplitaq Gold360, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold360/product/Thermo Fisher
    Average 99 stars, based on 22 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold360 - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    92
    GE Healthcare amplitaq
    Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for <t>AmpliTaq</t> Gold and “Platin” for Platinum Taq High Fidelity.
    Amplitaq, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq/product/GE Healthcare
    Average 92 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    amplitaq - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    PerkinElmer amplitaq
    Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for <t>AmpliTaq</t> Gold and “Platin” for Platinum Taq High Fidelity.
    Amplitaq, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 1085 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq/product/PerkinElmer
    Average 92 stars, based on 1085 article reviews
    Price from $9.99 to $1999.99
    amplitaq - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Roche amplitaq
    Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for <t>AmpliTaq</t> Gold and “Platin” for Platinum Taq High Fidelity.
    Amplitaq, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq/product/Roche
    Average 92 stars, based on 135 article reviews
    Price from $9.99 to $1999.99
    amplitaq - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Thermo Fisher amplitaq
    Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for <t>AmpliTaq</t> Gold and “Platin” for Platinum Taq High Fidelity.
    Amplitaq, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1571 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq/product/Thermo Fisher
    Average 92 stars, based on 1571 article reviews
    Price from $9.99 to $1999.99
    amplitaq - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Millipore amplitaq
    Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for <t>AmpliTaq</t> Gold and “Platin” for Platinum Taq High Fidelity.
    Amplitaq, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq/product/Millipore
    Average 92 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    amplitaq - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    TaKaRa amplitaq
    Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for <t>AmpliTaq</t> Gold and “Platin” for Platinum Taq High Fidelity.
    Amplitaq, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq/product/TaKaRa
    Average 92 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    amplitaq - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Promega amplitaq
    Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for <t>AmpliTaq</t> Gold and “Platin” for Platinum Taq High Fidelity.
    Amplitaq, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq/product/Promega
    Average 92 stars, based on 51 article reviews
    Price from $9.99 to $1999.99
    amplitaq - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    92
    Boehringer Mannheim amplitaq
    Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for <t>AmpliTaq</t> Gold and “Platin” for Platinum Taq High Fidelity.
    Amplitaq, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq/product/Boehringer Mannheim
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    amplitaq - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    88
    PerkinElmer amplitaq buffer
    Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for <t>AmpliTaq</t> Gold and “Platin” for Platinum Taq High Fidelity.
    Amplitaq Buffer, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 88/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq buffer/product/PerkinElmer
    Average 88 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    amplitaq buffer - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    88
    PerkinElmer amplitaq enzyme
    Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for <t>AmpliTaq</t> Gold and “Platin” for Platinum Taq High Fidelity.
    Amplitaq Enzyme, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 88/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq enzyme/product/PerkinElmer
    Average 88 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    amplitaq enzyme - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    88
    Thermo Fisher amplitaq enzyme
    Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for <t>AmpliTaq</t> Gold and “Platin” for Platinum Taq High Fidelity.
    Amplitaq Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq enzyme/product/Thermo Fisher
    Average 88 stars, based on 94 article reviews
    Price from $9.99 to $1999.99
    amplitaq enzyme - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    88
    PerkinElmer amplitaq fs
    Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for <t>AmpliTaq</t> Gold and “Platin” for Platinum Taq High Fidelity.
    Amplitaq Fs, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 88/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq fs/product/PerkinElmer
    Average 88 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    amplitaq fs - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    92
    PerkinElmer amplitaq gold
    Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for <t>AmpliTaq</t> Gold and “Platin” for Platinum Taq High Fidelity.
    Amplitaq Gold, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 92/100, based on 1665 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold/product/PerkinElmer
    Average 92 stars, based on 1665 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    93
    Roche amplitaq gold
    Effects of polymerase type and fluorescence-tagged primers on PCR–CRMS signal-to-noise ratio. Either <t>AmpliTaq</t> Gold or Expand enzyme was used to PCR amplify target DNAs. For each enzyme either an unlabeled or a FAM-labeled forward primer was used for PCR amplification. As in Figure 2, percent cleavage and MM:PM ratio are given below each reaction set.
    Amplitaq Gold, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 537 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq gold/product/Roche
    Average 93 stars, based on 537 article reviews
    Price from $9.99 to $1999.99
    amplitaq gold - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    92
    Thermo Fisher amplitaq mastermix
    Effects of polymerase type and fluorescence-tagged primers on PCR–CRMS signal-to-noise ratio. Either <t>AmpliTaq</t> Gold or Expand enzyme was used to PCR amplify target DNAs. For each enzyme either an unlabeled or a FAM-labeled forward primer was used for PCR amplification. As in Figure 2, percent cleavage and MM:PM ratio are given below each reaction set.
    Amplitaq Mastermix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq mastermix/product/Thermo Fisher
    Average 92 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    amplitaq mastermix - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    93
    Promega amplitaq polymerase
    Effects of polymerase type and fluorescence-tagged primers on PCR–CRMS signal-to-noise ratio. Either <t>AmpliTaq</t> Gold or Expand enzyme was used to PCR amplify target DNAs. For each enzyme either an unlabeled or a FAM-labeled forward primer was used for PCR amplification. As in Figure 2, percent cleavage and MM:PM ratio are given below each reaction set.
    Amplitaq Polymerase, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq polymerase/product/Promega
    Average 93 stars, based on 71 article reviews
    Price from $9.99 to $1999.99
    amplitaq polymerase - by Bioz Stars, 2020-05
    93/100 stars
      Buy from Supplier

    92
    Roche amplitaq polymerase
    Effects of polymerase type and fluorescence-tagged primers on PCR–CRMS signal-to-noise ratio. Either <t>AmpliTaq</t> Gold or Expand enzyme was used to PCR amplify target DNAs. For each enzyme either an unlabeled or a FAM-labeled forward primer was used for PCR amplification. As in Figure 2, percent cleavage and MM:PM ratio are given below each reaction set.
    Amplitaq Polymerase, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 325 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq polymerase/product/Roche
    Average 92 stars, based on 325 article reviews
    Price from $9.99 to $1999.99
    amplitaq polymerase - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    94
    Thermo Fisher amplitaq polymerase
    Effects of polymerase type and fluorescence-tagged primers on PCR–CRMS signal-to-noise ratio. Either <t>AmpliTaq</t> Gold or Expand enzyme was used to PCR amplify target DNAs. For each enzyme either an unlabeled or a FAM-labeled forward primer was used for PCR amplification. As in Figure 2, percent cleavage and MM:PM ratio are given below each reaction set.
    Amplitaq Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq polymerase/product/Thermo Fisher
    Average 94 stars, based on 1224 article reviews
    Price from $9.99 to $1999.99
    amplitaq polymerase - by Bioz Stars, 2020-05
    94/100 stars
      Buy from Supplier

    90
    Thermo Fisher amplitaq 360
    Effects of polymerase type and fluorescence-tagged primers on PCR–CRMS signal-to-noise ratio. Either <t>AmpliTaq</t> Gold or Expand enzyme was used to PCR amplify target DNAs. For each enzyme either an unlabeled or a FAM-labeled forward primer was used for PCR amplification. As in Figure 2, percent cleavage and MM:PM ratio are given below each reaction set.
    Amplitaq 360, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq 360/product/Thermo Fisher
    Average 90 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    amplitaq 360 - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    90
    Roche amplitaq goldtm
    Effects of polymerase type and fluorescence-tagged primers on PCR–CRMS signal-to-noise ratio. Either <t>AmpliTaq</t> Gold or Expand enzyme was used to PCR amplify target DNAs. For each enzyme either an unlabeled or a FAM-labeled forward primer was used for PCR amplification. As in Figure 2, percent cleavage and MM:PM ratio are given below each reaction set.
    Amplitaq Goldtm, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq goldtm/product/Roche
    Average 90 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    amplitaq goldtm - by Bioz Stars, 2020-05
    90/100 stars
      Buy from Supplier

    88
    PerkinElmer amplitaq ld
    Sensitivity of nested PCR amplification of E. coli NTCC 11151 DNA using primer pairs 16F plus 16R and NF plus NR. The template was E. coli NTCC 11151 DNA purified as described in the text. Lanes 1 to 8 contained the products of the amplification of 10 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, and 10 fg of DNA or no DNA using primers 16F and 16SR and <t>Amplitaq</t> LD that had been pretreated with Sau 3AI. Lane 9 contained the 1-kb size marker (Promega). Lanes 10 to 17 contain the products of the amplification of 1 μl of each of the first-round samples with primers NF and NR. Lane 18 contains a reagent control for the second round of amplification.
    Amplitaq Ld, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 88/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq ld/product/PerkinElmer
    Average 88 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    amplitaq ld - by Bioz Stars, 2020-05
    88/100 stars
      Buy from Supplier

    92
    Boehringer Mannheim amplitaq polymerase
    Sensitivity of nested PCR amplification of E. coli NTCC 11151 DNA using primer pairs 16F plus 16R and NF plus NR. The template was E. coli NTCC 11151 DNA purified as described in the text. Lanes 1 to 8 contained the products of the amplification of 10 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, and 10 fg of DNA or no DNA using primers 16F and 16SR and <t>Amplitaq</t> LD that had been pretreated with Sau 3AI. Lane 9 contained the 1-kb size marker (Promega). Lanes 10 to 17 contain the products of the amplification of 1 μl of each of the first-round samples with primers NF and NR. Lane 18 contains a reagent control for the second round of amplification.
    Amplitaq Polymerase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amplitaq polymerase/product/Boehringer Mannheim
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    amplitaq polymerase - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    Image Search Results


    Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for AmpliTaq Gold and “Platin” for Platinum Taq High Fidelity.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Patterns of nucleotide misincorporations during enzymatic amplification and direct large-scale sequencing of ancient DNA

    doi: 10.1073/pnas.0605327103

    Figure Lengend Snippet: Summary of the oligonucleotide competition amplifications. Each bar represents the ratio of nucleotides found among clones from amplification products generated from a mixture of two oligonucleotides, given by the abbreviations below the bars. Misincorporated nucleotides are indicated by the black upper part of each bar, and numbers give the numbers of clones sequenced. The polymerase reagents are indicated as “Gold” for AmpliTaq Gold and “Platin” for Platinum Taq High Fidelity.

    Article Snippet: These oligonucleotides were used as templates in PCRs with two DNA polymerase reagents, AmpliTaq Gold (Applied Biosystems, Foster City, CA) and Platinum Taq High Fidelity (Invitrogen, Carlsbad, CA), which are both commonly used in ancient DNA research.

    Techniques: Clone Assay, Amplification, Generated

    Effects of polymerase type and fluorescence-tagged primers on PCR–CRMS signal-to-noise ratio. Either AmpliTaq Gold or Expand enzyme was used to PCR amplify target DNAs. For each enzyme either an unlabeled or a FAM-labeled forward primer was used for PCR amplification. As in Figure 2, percent cleavage and MM:PM ratio are given below each reaction set.

    Journal: Nucleic Acids Research

    Article Title: PCR candidate region mismatch scanning: adaptation to quantitative, high-throughput genotyping

    doi:

    Figure Lengend Snippet: Effects of polymerase type and fluorescence-tagged primers on PCR–CRMS signal-to-noise ratio. Either AmpliTaq Gold or Expand enzyme was used to PCR amplify target DNAs. For each enzyme either an unlabeled or a FAM-labeled forward primer was used for PCR amplification. As in Figure 2, percent cleavage and MM:PM ratio are given below each reaction set.

    Article Snippet: AmpliTaq Gold was purchased from Roche Pharmaceuticals.

    Techniques: Fluorescence, Polymerase Chain Reaction, Labeling, Amplification

    Sensitivity of nested PCR amplification of E. coli NTCC 11151 DNA using primer pairs 16F plus 16R and NF plus NR. The template was E. coli NTCC 11151 DNA purified as described in the text. Lanes 1 to 8 contained the products of the amplification of 10 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, and 10 fg of DNA or no DNA using primers 16F and 16SR and Amplitaq LD that had been pretreated with Sau 3AI. Lane 9 contained the 1-kb size marker (Promega). Lanes 10 to 17 contain the products of the amplification of 1 μl of each of the first-round samples with primers NF and NR. Lane 18 contains a reagent control for the second round of amplification.

    Journal: Journal of Clinical Microbiology

    Article Title: Elimination of Bacterial DNA from Taq DNA Polymerases by Restriction Endonuclease Digestion

    doi:

    Figure Lengend Snippet: Sensitivity of nested PCR amplification of E. coli NTCC 11151 DNA using primer pairs 16F plus 16R and NF plus NR. The template was E. coli NTCC 11151 DNA purified as described in the text. Lanes 1 to 8 contained the products of the amplification of 10 ng, 1 ng, 100 pg, 10 pg, 1 pg, 100 fg, and 10 fg of DNA or no DNA using primers 16F and 16SR and Amplitaq LD that had been pretreated with Sau 3AI. Lane 9 contained the 1-kb size marker (Promega). Lanes 10 to 17 contain the products of the amplification of 1 μl of each of the first-round samples with primers NF and NR. Lane 18 contains a reagent control for the second round of amplification.

    Article Snippet: Amplitaq LD from Perkin-Elmer, an ultrapure preparation, is guaranteed to contain less than 10 copies of bacterial 16S rRNA gene sequences per 2.5-μl aliquot, which was sufficient to produce a false-positive result under our conditions of amplification.

    Techniques: Nested PCR, Amplification, Purification, Marker

    Nested PCRs using primer pairs 16F plus 16R and NR plus NF amplify a product in the absence of an added template. Lanes 1 to 5 were amplified by using Amplitaq LD (Perkin-Elmer, Cheshire, United Kingdom), lanes 6 to 10 were amplified with Amplitaq (Perkin-Elmer), and lanes 11 to 15 were amplified with Taq DNA polymerase (Stratagene, Amsterdam, The Netherlands). Lanes 1, 6, and 11 were positive controls for the outer PCRs; lanes 2, 7, and 12 were reagent controls for the outer reaction; lanes 3, 8, and 13 were positive controls for the nested reaction; lanes 4, 9, and 14 contained 1 μl of the first-round reagent control amplified with the nested primers; and lanes 5, 10, and 15 were reagent controls for the nested PCR. Lane 16 contained the molecular size marker (Promega, Wis.).

    Journal: Journal of Clinical Microbiology

    Article Title: Elimination of Bacterial DNA from Taq DNA Polymerases by Restriction Endonuclease Digestion

    doi:

    Figure Lengend Snippet: Nested PCRs using primer pairs 16F plus 16R and NR plus NF amplify a product in the absence of an added template. Lanes 1 to 5 were amplified by using Amplitaq LD (Perkin-Elmer, Cheshire, United Kingdom), lanes 6 to 10 were amplified with Amplitaq (Perkin-Elmer), and lanes 11 to 15 were amplified with Taq DNA polymerase (Stratagene, Amsterdam, The Netherlands). Lanes 1, 6, and 11 were positive controls for the outer PCRs; lanes 2, 7, and 12 were reagent controls for the outer reaction; lanes 3, 8, and 13 were positive controls for the nested reaction; lanes 4, 9, and 14 contained 1 μl of the first-round reagent control amplified with the nested primers; and lanes 5, 10, and 15 were reagent controls for the nested PCR. Lane 16 contained the molecular size marker (Promega, Wis.).

    Article Snippet: Amplitaq LD from Perkin-Elmer, an ultrapure preparation, is guaranteed to contain less than 10 copies of bacterial 16S rRNA gene sequences per 2.5-μl aliquot, which was sufficient to produce a false-positive result under our conditions of amplification.

    Techniques: Amplification, Nested PCR, Marker