Journal: BMC biology

Article Title: Lipid metabolites affected by deficient autophagy antagonize the occurrence of autophagy through AMPK signaling in insects.

doi: 10.1186/s12915-025-02274-z

Figure Lengend Snippet: Fig. 7 Effects of metabolites on autophagy occurrence in Drosophila fat body and B. mori BmN cells. A and B LysoTracker Red staining (A), western blots and protein quantification of BmAtg8–PE and p-AMPKα (T172) (B) of Drosophila fat body at W stage after treatment with 40 μM LPE(19:0), LPC(0:0/17:0), LPC(0:0/19:0), or LPC(20:0/0:0) for 2 h, individually. C LysoTracker Red staining from nutritious and starved BmN cells after treatment with LPE(19:0), LPC(0:0/17:0), LPC(0:0/19:0), or LPC(20:0/0:0) (40 μM) for 2 h (N, normal nutrient; S, starvation). D-G Western blots and protein quantification of BmAtg8–PE and p-AMPKα (T172) after treatment with 40 μM LPE(19:0) (D), LPC(0:0/17:0) (E), LPC(0:0/19:0) (F), or LPC(20:0/0:0) (G) for 2 h in normal-nutrition and starved BmN cells (S, starvation). The solvent PBS was used as control. Data are presented as means ± SD; P values were determined by unpaired two-tailed Student’s t-test. Significance test was performed between control and each metabolite treatment. *P < 0.05, **P < 0.01, ***P < 0.001. ns, no significant difference

Article Snippet: The primary antibodies for V5 (Cell Signaling Technology, 13202S; 1:3000), FLAG (Cell Signaling Technology, 14793S; 1:3000), HA (Santa Cruz Biotechnology, sc-7392; 1:2000), BmAtg8–PE (Abcam, ab109364; 1:4000), Phospho-AMPKα (Thr172) (Cell Signaling Technology, 50081; 1:3000), and Tubulin (Beyotime Biotechnology, AT819; 1:5000) were used for western blotting according to the standard procedure as previously described [17, 41].

Techniques: Staining, Western Blot, Solvent, Control, Two Tailed Test

Journal: Mitochondrion

Article Title: Transplantation of astrocyte-derived mitochondria into injured astrocytes has a protective effect following stretch injury.

doi: 10.1016/j.mito.2024.101902

Figure Lengend Snippet: Fig. 7. Mitochondrial transplantation suppresses activation of the IKK/NF-κB pathway and accelerates activation of the AMPK/PGC-1α pathway after SI. (A) Representative blots of p-IKKα, IKKα, p-NF-κB, NF-κB, p-AMPKα, AMPKα, and PGC-1α and (B) statistical analysis (n = 3). (C) Statistical analysis of relative mtDNA copy number (n = 4). *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: Primary antibodies against the following factors were used: β-actin (1: 2000, Cat# 66009–1-Ig, Proteintech, Rosemont, IL, USA), β-tubulin (1: 2000, Cat# MA5–16308, Thermo Fisher Scientific), phospho-Drp1 (p-Drp1, 1: 1000, Cat# 3455, CST), Drp1(1: 1000, Cat# 8570, CST), Opa1 (1: 1000, Cat# 80471, CST), Mfn2 (1: 1000, Cat# 9482, CST), cleaved-caspase 9 (cl-caspase9, 1: 500, Cat# 9509, CST), caspase 9 (1: 1000, Cat# 9504, CST), Bcl-2 (1: 1000, Cat# 3498, CST), Bax (1: 1000, Cat# 2772, CST), phospho-IKKα/β (p-IKKα/β, 1: 1000, Cat# 2697, CST), IKKα (1: 1000, Cat# 11930, CST), phosphoNF-κB (p-NF-κB, 1: 1000, Cat# 3033, CST), NF-κB (1: 1000, Cat# 8242, CST), phospho-AMPKα (p-AMPKα, 1: 1000, Cat# 2535, CST), AMPKα (1: 1000, Cat# 5832, CST), and PGC-1α (1: 10000, Cat# 66369–1-Ig, Proteintech), as well as anti-mouse (1: 5000, Cat# 7076, CST), and antirabbit (1: 5000, Cat# 7074, CST) horseradish peroxidase-conjugated secondary antibodies.

Techniques: Transplantation Assay, Activation Assay