Journal: Cell reports

Article Title: CaMKII binds both substrates and activators at the active site

doi: 10.1016/j.celrep.2022.111064

Figure Lengend Snippet:

Article Snippet: AMPPNP , Tocris , CAT# 6086; CAS# 72957-42-7.

Techniques: Virus, Recombinant, Protease Inhibitor, FLAG-tag, Software

Journal: bioRxiv

Article Title: Dual Pathways of Extracellular ATP Action in Cancer Cells: Purinergic Signaling–Driven Senescence and Macropinocytic ATP Internalization

doi: 10.64898/2026.04.23.720363

Figure Lengend Snippet: (A–B) Short-time interval ATP concentration assay (minutes). Intracellular ATP (iATP, % of control) for A549 (A) and A549STC1KO (B) cells measured at the indicated induction times between 30-120 minutes. (C–D) Long-time interval ATP concentration assay (hours). iATP (%) for A549 WT (C) and STC1-KO (D) across induction times between 2-4 hours. (E) Immunofluorescence/confocal imaging of macropinocytosis. A549 WT, A549 STC1-KO (two independent clones), and NL20 control cells were stained with NHF-ATP (green; ATP analog), High molecular weight dextran (red-macropinosome marker), and DAPI (blue; cell nuclei). Merged images show colocalization (yellow). STC1-KO cells display visibly reduced FITC-dextran uptake relative to WT based on gene expression reduction, while NL20 shows minimal uptake. Scale bars: 20 µm (A549 and STC1-KO), 10 µm (NL20). Data is representation for one set of biological replicates as mean ± SD for (n ≥ 5 technical replicates). Colors denote the eATP dose as indicated in the in-plot legend. One-way ANOVA with post-hoc multiple-comparisons; *P < 0.05,**P < 0.01,***P < 0.001, ****P < 0.0001.

Article Snippet: For fluorescence microscopy, NHF-ATP was obtained from Jena Bioscience (NU-810-488), ProLong TM Gold Antifade Mountant with DAPI from Thermo Fisher and high molecular weight fluorescent TMR-dextran (HMWFD) 70,000 Daltons MW, Neutral (no electric charge) from Invitrogen (D818).

Techniques: Concentration Assay, Control, Immunofluorescence, Imaging, Clone Assay, Staining, High Molecular Weight, Marker, Gene Expression

Journal: bioRxiv

Article Title: Dual Pathways of Extracellular ATP Action in Cancer Cells: Purinergic Signaling–Driven Senescence and Macropinocytic ATP Internalization

doi: 10.64898/2026.04.23.720363

Figure Lengend Snippet: Tumor xenografts were established by subcutaneous injection of 1×10 –5×10 cells into the right flank of 3–4-week-old male NU/J nude mice. When tumors reached ∼200–500 mm³, tumors were injected intratumorally with DMEM control, HMWFD tracer, or HMWFD plus NHF-ATP (100 μM) in a total volume of 50 μL using 27G needles. Tumors were harvested within ∼7–8 minutes post-injection, embedded in OCT, and cryosectioned (10 μm). Sections were ethanol-fixed, PBS-washed, and mounted with antifade medium containing DAPI. Images were acquired using a Nikon NiU epifluorescence microscope with identical exposure settings across groups and processed using Nikon NIS-Elements software. A-H. NHF-ATP and HMWFD colocalize in HT29 (A), PANC1 (B), MCF7 (C), SK-HEP1 (D), HOP-92 (E), H1299 (F), and A549 (G) and A375 (H). respectively. Color: Blue:DAPI (nuclei), Red: HMWFD, Green: NHF-ATP, Yellow: Co-localization. Scale bar: 10 µm, 100x magnification.

Article Snippet: For fluorescence microscopy, NHF-ATP was obtained from Jena Bioscience (NU-810-488), ProLong TM Gold Antifade Mountant with DAPI from Thermo Fisher and high molecular weight fluorescent TMR-dextran (HMWFD) 70,000 Daltons MW, Neutral (no electric charge) from Invitrogen (D818).

Techniques: Injection, Control, Microscopy, Software