Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Quorum-quenching enzyme Est816 assisted antibiotics against periodontitis induced by Aggregatibacter actinomycetemcomitans in rats

doi: 10.3389/fcimb.2024.1368684

Figure Lengend Snippet: Minimum inhibitory concentration (MIC) results for A. actinomycetemcomitans of (A) Minocycline, (B) Metronidazole and (C) Amoxicillin. The antibiotic concentrations ranged from 0.125 to 128 μg/mL. For the inactivated enzyme group, the enzyme was inactivated by heating. The error bars in each panel represent the SD of triplicates.

Article Snippet: 50 μL of the bacterial suspension (1× 10 6 CFU/mL) (OD 600 = 0.1) was added to equal volume of metronidazole (MTZ), amoxicillin (AMX), and minocycline (MINO) on the 96-well plate (MedChemExpress, NJ, U.S.A.) ( ).

Techniques: Concentration Assay

Journal: Frontiers in Cellular and Infection Microbiology

Article Title: Quorum-quenching enzyme Est816 assisted antibiotics against periodontitis induced by Aggregatibacter actinomycetemcomitans in rats

doi: 10.3389/fcimb.2024.1368684

Figure Lengend Snippet: Morphology and biofilm formation of A . actinomycetemcomitans . (A) SEM revealed the effect of 12 U/mL lactonase Est816 with and without antibiotics on A . actinomycetemcomitans biofilm (original magnification × 1,000 and × 5,000). (B) Biofilm inhibition rate of minocycline (MINO), metronidazole (MTX) and amoxicillin (AMX) alone and in combination with enzyme Est816. (C) Biofilm eradication rate of MINO, MTZ, and AMX alone and in combination with enzyme Est816. (Compare within groups, ** P <0.01, *** P <0.001).

Article Snippet: 50 μL of the bacterial suspension (1× 10 6 CFU/mL) (OD 600 = 0.1) was added to equal volume of metronidazole (MTZ), amoxicillin (AMX), and minocycline (MINO) on the 96-well plate (MedChemExpress, NJ, U.S.A.) ( ).

Techniques: Inhibition

Journal: Microbiology Spectrum

Article Title: Unlocking the mechanism of action: a cost-effective flow cytometry approach for accelerating antimicrobial drug development

doi: 10.1128/spectrum.03931-23

Figure Lengend Snippet: Antimicrobial compounds with their respective final concentration and supplier, grouped by mechanism of action

Article Snippet: Amoxicillin , 0.01 mg/mL , LKT Laboratories Inc., St. Paul, Minnesota, USA.

Techniques: Concentration Assay, Membrane, Disruption, Inhibition

Journal: Scientific Reports

Article Title: In Vitro Micropatterned Human Pluripotent Stem Cell Test (µP-hPST) for Morphometric-Based Teratogen Screening

doi: 10.1038/s41598-017-09178-1

Figure Lengend Snippet: Compound screening results in µP-hPST. The C max values of each compound were mainly acquired from U.S. Food and Drug Administration website, European Medicines Agency website or published journals unless otherwise noted. The full list could be found in Supplementary Table S1 . NON: Non-teratogen, TER: Teratogen, NA: not applicable.

Article Snippet: All compounds used in this study were purchased from Selleck Chemicals or Sigma-Aldrich: 5-Fluorouracil (F6627,Sigma-Aldrich), Acetaminophen (A7085, Sigma-Aldrich), Acyclovir (S1807, Selleck Chemicals), Amoxicillin (S3015, Selleck Chemicals), Ascorbic acid (A5960, Sigma-Aldrich), Bosentan (S4220, Selleck Chemicals), Busulfan (B2635, Sigma-Aldrich), Caffeine (27600, Sigma-Aldrich), Carbamazepine (C8981, Sigma-Aldrich), Diethylstilbestrol (D4528, Sigma-Aldrich), Diphenhydramine (S1866, Selleck Chemicals), Diphenylhydantoin (D4007, Sigma-Aldrich), Doxorubicin (S1208, Selleck Chemicals), Doxylamine (D3775, Sigma-Aldrich), Esomeprazole (S1743, Selleck Chemicals), Folic acid (F7876, Sigma-Aldrich), Furosemide (F4381, Sigma-Aldrich), Gefitinib (S1025, Selleck Chemicals), Imatinib (S1026, Selleck Chemicals), Isoniazid (S1937, Selleck Chemicals), Loratadine (S1358, Selleck Chemicals), Lovastatin (S2061, Selleck Chemicals), Methimazole (S1609, Selleck Chemicals), Methyldopa (S1642, Selleck Chemicals), Metoclopramide (M0763, Sigma-Aldrich), Sitagliptin (S4002, Selleck Chemicals), Sunitinib (S1042, Selleck Chemicals), Vandetanib (S1046, Selleck Chemicals), Thiamine (T4625, Sigma-Aldrich), Ziprasidone (S1444, Selleck Chemicals).

Techniques: In Vitro, In Vivo

Journal: Scientific Reports

Article Title: In Vitro Micropatterned Human Pluripotent Stem Cell Test (µP-hPST) for Morphometric-Based Teratogen Screening

doi: 10.1038/s41598-017-09178-1

Figure Lengend Snippet: Description of test compounds. The chemical properties and usages of drugs were found in PubChem Open Chemistry Database ( https://pubchem.ncbi.nlm.nih.gov ). NON: Non-teratogen, TER: Teratogen.

Article Snippet: All compounds used in this study were purchased from Selleck Chemicals or Sigma-Aldrich: 5-Fluorouracil (F6627,Sigma-Aldrich), Acetaminophen (A7085, Sigma-Aldrich), Acyclovir (S1807, Selleck Chemicals), Amoxicillin (S3015, Selleck Chemicals), Ascorbic acid (A5960, Sigma-Aldrich), Bosentan (S4220, Selleck Chemicals), Busulfan (B2635, Sigma-Aldrich), Caffeine (27600, Sigma-Aldrich), Carbamazepine (C8981, Sigma-Aldrich), Diethylstilbestrol (D4528, Sigma-Aldrich), Diphenhydramine (S1866, Selleck Chemicals), Diphenylhydantoin (D4007, Sigma-Aldrich), Doxorubicin (S1208, Selleck Chemicals), Doxylamine (D3775, Sigma-Aldrich), Esomeprazole (S1743, Selleck Chemicals), Folic acid (F7876, Sigma-Aldrich), Furosemide (F4381, Sigma-Aldrich), Gefitinib (S1025, Selleck Chemicals), Imatinib (S1026, Selleck Chemicals), Isoniazid (S1937, Selleck Chemicals), Loratadine (S1358, Selleck Chemicals), Lovastatin (S2061, Selleck Chemicals), Methimazole (S1609, Selleck Chemicals), Methyldopa (S1642, Selleck Chemicals), Metoclopramide (M0763, Sigma-Aldrich), Sitagliptin (S4002, Selleck Chemicals), Sunitinib (S1042, Selleck Chemicals), Vandetanib (S1046, Selleck Chemicals), Thiamine (T4625, Sigma-Aldrich), Ziprasidone (S1444, Selleck Chemicals).

Techniques: In Vivo

Journal: ACS sensors

Article Title: Paper-Based Enzyme Competition Assay for Detecting Falsified β-Lactam Antibiotics.

doi: 10.1021/acssensors.8b00163

Figure Lengend Snippet: Figure 4. Calibration curves of common antibiotics including (A) Ampicillin (m error = 4.75, b error = 2.64) and (B) Amoxicillin (m error = 4.17, b error = 2.51) (n = 3).

Article Snippet: In addition to ampicillin, calibration curves were also generated for amoxicillin sodium salt [Alfa Aesar, >88% purity), penicillin V potassium salt [Acros Organics, >99% purity], and cefazolin sodium salt [Chem Impex Int’l Inc., >93% purity].

Techniques:

Journal: Ecotoxicology and environmental safety

Article Title: Mechanistic and ecotoxicological studies of amoxicillin removal through anaerobic degradation systems.

doi: 10.1016/j.ecoenv.2020.110207

Figure Lengend Snippet: Fig. 1. Precursor and product ions obtained for amoxicillin and its by-products identified by LC-HRMS analysis.

Article Snippet: Tests were conducted to determine the anaerobic biodegradation of amoxicillin (Acros, 96%) according to ISO 11734 (1995) with 100 mL glass syringes filled with synthetic effluent containing the target compound AMX.

Techniques: