Journal: Journal of pharmacological sciences

Article Title: Long-lasting pain-related behaviors in mouse chronic cystitis model induced by a single intravesical injection of hydrogen peroxide.

doi: 10.1016/j.jphs.2015.11.003

Figure Lengend Snippet: Fig. 2. Bladder distension-evoked licking to lower abdomen in H2O2-induced cystitis model. (A) Mice were injected intravesically (i.ves.) with saline or 1.5% H2O2. 1, 7 and 14 days after the injection, mice were injected with 50 mL of saline to dilate bladder. After the recovery from anesthesia, the time spent licking of the lower abdomen was measured for 20 min n ¼ 6e15. *P < 0.05, **P < 0.01, compared with i.ves. saline- injected control mice. (B) Effects of therapeutic drugs on the increased total licking time in H2O2-induced cystitis model mice. Seven days after the i.ves. saline or 1.5% H2O2 injection, oxybutynin (3 mg/kg), indomethacin (3 mg/kg), amitriptyline (1 mg/ kg), morphine (3 mg/kg) or vehicle was given intraperitoneally 30 min before testing. The licking time was measured for 20 min n ¼ 7e13. ***P < 0.001, N.S. ¼ not significant.

Article Snippet: To examine the effects of drugs, oxybutynin chloride, indomethacin (SigmaeAldrich Japan, Tokyo), amitriptyline hydrochloride (LKT Laboratories Inc., USA) or morphine hydrochloride (Takeda Pharmaceutical Co., Osaka) was freshly dissolved in a saline containing 5% DMSO and 2% Tween80.

Techniques: Injection, Saline, Control