Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Dysregulation of miR-342-3p in plasma exosomes derived from convalescent AMI patients and its consequences on cardiac repair.

doi: 10.1016/j.biopha.2021.112056

Figure Lengend Snippet: Fig. 1. Characterization and internalization of Nor-Exo and AMI-Exo. (A) Electron microscopic images of isolated exosomes. Scale bar: 200 nm. (B) Results of nanoparticle tracking analysis of exosomes. (C) The Alix and CD63 expression levels in both Nor-Exo and AMI-Exo are shown in western blots and presented as a bar graph (n = 3). (D) Representative fluorescent micrographs showing Nor-Exo and AMI-Exo are internalized by H9c2 cells. Red: PKH26-labeled exosomes, blue: DAPI. Scale bar: 20 µm.

Article Snippet: To verify the role of miR-342-3p in exosome-mediated heart repair, we transfected miR-342-3p mimic or negative control into AMI-Exo by electroporation and evaluated the therapeutic effects of mimic NC-AMI Exo and miR-342-3p mimic-AMI Exo in the AMI rat model. At 28 d postMI, rats injected with miR-342-3p mimic-AMI Exo had a decreased infarct area (Fig. 6A and B) and improved heart function (Fig. 6C–E), compared to the hearts injected with mimic NC-AMI Exo.

Techniques: Isolation, Expressing, Western Blot, Labeling

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Dysregulation of miR-342-3p in plasma exosomes derived from convalescent AMI patients and its consequences on cardiac repair.

doi: 10.1016/j.biopha.2021.112056

Figure Lengend Snippet: Fig. 2. Effects of Nor-Exo and AMI-Exo treatment on H9c2 cardiomyocytes subjected to H2O2. (A-C) H9c2 cells were pretreated with different concentrations of Nor- Exo or AMI-Exo (50 μg/mL, 100 μg/mL, and 200 μg/mL) for 4 h and exposed to H2O2 (100 μM) for 4 h. Cell viability was measured using CCK-8 assay (A) and apoptosis rate was quantified (B-C) by flow cytometry (n = 3). (D-E) H9c2 cells were pretreated with Nor-Exo or AMI-Exo (100 μg/mL) for 4 h and exposed to H2O2 (100 μM) for 4 h. Bax and Bcl-2 levels were examined by western blot analysis and are presented as a bar graph (n = 3). (F-G) H9c2 cells were treated as shown in (D). TUNEL staining was used to detect apoptotic cells (red), and the percentage of apoptotic cells was quantified (n = 5 random fields per group). Blue: DAPI, green: α-Sarcomeric actin. Scale bar, 100 µm. The data are expressed as the mean ± SEM. *P＜0.05, compared to the Control group; #P＜0.05, compared to the H2O2 group; &P＜0.05, compared to the AMI-Exo+ H2O2 group.

Article Snippet: To verify the role of miR-342-3p in exosome-mediated heart repair, we transfected miR-342-3p mimic or negative control into AMI-Exo by electroporation and evaluated the therapeutic effects of mimic NC-AMI Exo and miR-342-3p mimic-AMI Exo in the AMI rat model. At 28 d postMI, rats injected with miR-342-3p mimic-AMI Exo had a decreased infarct area (Fig. 6A and B) and improved heart function (Fig. 6C–E), compared to the hearts injected with mimic NC-AMI Exo.

Techniques: CCK-8 Assay, Flow Cytometry, Western Blot, TUNEL Assay, Staining, Control

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Dysregulation of miR-342-3p in plasma exosomes derived from convalescent AMI patients and its consequences on cardiac repair.

doi: 10.1016/j.biopha.2021.112056

Figure Lengend Snippet: Fig. 3. Effects of Nor-Exo and AMI-Exo treatment in a rat model of AMI. (A) The flowchart of experimental design in vivo. (B) Electrocardiogram of rats before and after LAD ligation. (C) Representative Masson’s trichome-stained myocardial sections at 28 d from MI rats treated with sham, PBS, Nor-Exo, or AMI-Exo. Scale bar: 1 mm. (D) Quantitative analyses of infarct area from Masson’s trichrome-stained heart sections (n = 8). (E-F) Quantification of EF%, FS% measured by echocar diography of Sham, PBS-treated, Nor-Exo-treated, and AMI-Exo-treated rats at 28 d post-MI (n = 6). (G) Representative echocardiographic images at 28 d post-MI.

Article Snippet: To verify the role of miR-342-3p in exosome-mediated heart repair, we transfected miR-342-3p mimic or negative control into AMI-Exo by electroporation and evaluated the therapeutic effects of mimic NC-AMI Exo and miR-342-3p mimic-AMI Exo in the AMI rat model. At 28 d postMI, rats injected with miR-342-3p mimic-AMI Exo had a decreased infarct area (Fig. 6A and B) and improved heart function (Fig. 6C–E), compared to the hearts injected with mimic NC-AMI Exo.

Techniques: In Vivo, Ligation, Staining

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Dysregulation of miR-342-3p in plasma exosomes derived from convalescent AMI patients and its consequences on cardiac repair.

doi: 10.1016/j.biopha.2021.112056

Figure Lengend Snippet: Fig. 4. miR-342-3p was dysregulated in AMI-Exo. (A) miRNA profiling assays were performed in Nor-Exo (n = 3) and AMI-Exo (n = 3). The heat map shows the 10 most upregulated and 10 most downregulated miRNAs in AMI-Exo group. (B) qRT-PCR analysis of miR-193a-5p, miR-193b-5p, miR-23b-5p, miR-342-3p, and miR- 1228-5p between Nor-Exo and AMI-Exo (n = 3 independent experiments). *P＜0.05, compared to the Nor-Exo group. (C) H9c2 cells were transfected with a mimic of indicated miRNA or mimic-NC for 48 h and exposed to H2O2 (100 μM) for 4 h. Cell viability was measured using CCK-8 assay. The data are expressed as the mean ± SEM. *P＜0.05, compared to the Control group; #P＜0.05, compared to the mimic-NC+ H2O2 group.

Article Snippet: To verify the role of miR-342-3p in exosome-mediated heart repair, we transfected miR-342-3p mimic or negative control into AMI-Exo by electroporation and evaluated the therapeutic effects of mimic NC-AMI Exo and miR-342-3p mimic-AMI Exo in the AMI rat model. At 28 d postMI, rats injected with miR-342-3p mimic-AMI Exo had a decreased infarct area (Fig. 6A and B) and improved heart function (Fig. 6C–E), compared to the hearts injected with mimic NC-AMI Exo.

Techniques: Quantitative RT-PCR, Transfection, CCK-8 Assay, Control

Journal: STAR Protocols

Article Title: Protocol for deep brain stimulation in the fimbria-fornix of freely moving mice

doi: 10.1016/j.xpro.2021.101054

Figure Lengend Snippet:

Article Snippet: Audio interface for ANY-maze , Stoelting , AMi-2.

Techniques: Recombinant, Saline, Ointment, Adhesive, Software, Dissection, Microscopy