Journal: Journal of Biological Engineering

Article Title: A new approach to off-gas analysis for shaken bioreactors showing high CTR and RQ accuracy

doi: 10.1186/s13036-025-00480-5

Figure Lengend Snippet: Comparison of differential pressure sensor (∆p sensor) and non-dispersive infrared CO sensor (NDIR CO sensor) for CTR measurement. CTR over time determined with ∆p sensor (red) (as described by Anderlei et al. and NDIR CO sensor (green). For comparison of both methods, a ∆p sensor was included in the measurement setup as illustrated in Additional file B. The open vent hole was replaced by a switchable outlet valve, to allow for gas tightness. Data points are presented as duplicate measurements in two individual shake flasks (square and triangle). The solid line connects the average of these two data points. A Saccharomyces cerevisiae ( S. cerevisiae ) cultivation in yeast extract peptone medium (YEP-medium) containing 20 g/L glucose. Operation conditions: shaking frequency n = 200 rpm, shaking diameter d 0 = 50 mm, liquid filling volume V L = 25 mL, 250 mL shake flask, temperature T = 30 °C, measurement time t meas = 6 min; B Gluconobacter oxydans ( G. oxydans ) in Jülich-medium containing 80 g/L mannitol. Operation conditions: n = 350 rpm, d 0 = 50 mm, V L = 10 mL, 250 mL shake flask, T = 30 °C, t meas = 6 min

Article Snippet: A recycle pump (mp6, Bartels, Germany) continuously withdraws gas from the flask headspace and passes this gas through an a non-dispersive infrared (NDIR) CO 2 sensor (Alphasense Ltd., UK) and electrochemical oxygen sensor (Maxtec, US), before recycling the gas back to the flask headspace (blue path in Fig. A and B).

Techniques: Comparison

Journal: Journal of Biological Engineering

Article Title: A new approach to off-gas analysis for shaken bioreactors showing high CTR and RQ accuracy

doi: 10.1186/s13036-025-00480-5

Figure Lengend Snippet: Comparison of respiration measurement using an open vent hole or an outlet valve. OTR, CTR and RQ was determined for S. cerevisiae and G. oxydans cultivations in a TOM setup with open vent hole (as in Fig. ) and with outlet valve (as shown in Additional file B) for validation of the former. Results including outlet valve are presented as green lines and closed green triangles and squares. Results with open vent hole are presented as solid orange lines and solid orange circles and diamonds. Neglection of the convective flow through the open vent hole in the CO and O balance leads to results shown as dashed orange line and open orange circles and diamonds. NDIR CO sensors were used for CTR and RQ measurement in both setups. Data points are presented as duplicate measurements in two individual shake flasks. The lines connect the average of these two data points. A , B , C S. cerevisiae cultivation in YEP-medium containing 20 g/L glucose. Operation conditions: n = 200 rpm, d 0 = 50 mm, V L = 25 mL, 250 mL shake flask, T = 30 °C; in C RQ = 1 is indicated as horizontal dashed line. D , E , F G. oxydans in Jülich-medium containing 80 g/L mannitol. Operation conditions: n = 350 rpm, d 0 = 50 mm, V L = 10 mL, 250 mL shake flask, T = 30 °C, t meas = 6 min

Article Snippet: A recycle pump (mp6, Bartels, Germany) continuously withdraws gas from the flask headspace and passes this gas through an a non-dispersive infrared (NDIR) CO 2 sensor (Alphasense Ltd., UK) and electrochemical oxygen sensor (Maxtec, US), before recycling the gas back to the flask headspace (blue path in Fig. A and B).

Techniques: Comparison, Biomarker Discovery

Journal: Journal of Biological Engineering

Article Title: A new approach to off-gas analysis for shaken bioreactors showing high CTR and RQ accuracy

doi: 10.1186/s13036-025-00480-5

Figure Lengend Snippet: Comparison of different technologies for off-gas analysis in shake flasks

Article Snippet: A recycle pump (mp6, Bartels, Germany) continuously withdraws gas from the flask headspace and passes this gas through an a non-dispersive infrared (NDIR) CO 2 sensor (Alphasense Ltd., UK) and electrochemical oxygen sensor (Maxtec, US), before recycling the gas back to the flask headspace (blue path in Fig. A and B).

Techniques: Comparison, Fluorescence

Journal: Sensors (Basel, Switzerland)

Article Title: Development and Validation of a UAV Based System for Air Pollution Measurements

doi: 10.3390/s16122202

Figure Lengend Snippet: Queensland University of Technology (QUT) payload sensor system capable of measuring CO 2 , CO , NO 2 , NO .

Article Snippet: The gas sensing payload includes three Alphasense gas sensors (Alphasense, B4 type, Great Notley, Essex, UK) and one SprintIR CO 2 sensor.

Techniques:

Journal: Sensors (Basel, Switzerland)

Article Title: Development and Validation of a UAV Based System for Air Pollution Measurements

doi: 10.3390/s16122202

Figure Lengend Snippet: Test 2 ground measurements summary.

Article Snippet: The gas sensing payload includes three Alphasense gas sensors (Alphasense, B4 type, Great Notley, Essex, UK) and one SprintIR CO 2 sensor.

Techniques: